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rabbit anti-GFAP polyclonal antibody 2539

(100 customer reviews)

$100.00$2,600.00

Antibody summary

  • Rabbit polyclonal to GFAP
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: human, mouse, rat
  • Isotype: IgG
  • 100 µg
SKU: 2539parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

chicken

isotype

IgY

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, IHC, WB

reactivity

human, mouse, rat

available sizes

1 mg, 100 µg, 25 µg

rabbit anti-GFAP polyclonal antibody 2539

antibody
Database link:
human P14136
mouse P03995
rat P47819
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
WB: 1:5000 IF/ICC and IHC 1:1000-1,5000
Immunogen
Recombinant full length human GFAP isotype 1 expressed in and purified from E. coli.
Size and concentration
25, 100, 1000µL and serum
Form
liquid
Storage Instructions
2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer
serum, 0.04% NaN3 added
Purity
serum
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Glial Fibrillary Acidic Protein (GFAP) is a crucial cell marker widely employed in neuroscience and neurobiology research. It is an intermediate filament protein primarily found in astrocytes, a type of glial cell in the central nervous system. GFAP serves as a reliable marker for identifying and characterizing astrocytes in various experimental models and tissues. Immunohistochemistry and immunofluorescence techniques using antibodies targeting GFAP enable researchers to visualize and quantify astrocytic populations, gaining insights into their distribution, morphology, and function in the brain. Moreover, GFAP antibodies are instrumental in studying astrocyte reactivity, a phenomenon occurring in response to brain injury or neurodegenerative diseases. As astrocytes play critical roles in neural development, neurotransmitter regulation, and maintenance of the blood-brain barrier, GFAP has proven indispensable in advancing our understanding of glial biology and its contribution to neurological disorders.

Click for more on: cell markers and GFAP
Protein names
Glial fibrillary acidic protein (GFAP)
Gene names
GFAP,GFAP
Protein family
Intermediate filament family
Mass
49880Da
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Subellular location
Cytoplasm Note=Associated with intermediate filaments.
Tissues
Expressed in cells lacking fibronectin.
Structure
Interacts with SYNM. ; [Isoform 2]: Interacts with PSEN1 (via N-terminus).
Post-translational modification
Phosphorylated by PKN1.
Involvement in disease
Alexander disease (ALXDRD) [MIM:203450]: A rare disorder of the central nervous system. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death within the first decade. Infants with Alexander disease develop a leukodystrophy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. Histologically, Alexander disease is characterized by Rosenthal fibers, homogeneous eosinophilic inclusions in astrocytes. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P14136
The UniProt Consortium

Data

ICC/IF-image-rabbit-anti-GFAP-polyclonal-antibody-2539
Immunofluorescent analysis of a rat cerebellum section stained with rabbit pAb to GFAP, 2539, dilution 1:5,000 in green and costained with mouse mAb to MeCP2 dilution 1:500, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45µM, and free-floating sections were stained with above antibodies. The GFAP antibody stains the network of astrocytic cells and the processes of Bergmann glia in the molecular layer. The MeCP2 antibody specifically labels nuclei of certain neurons.
IHC-image-rabbit-anti-GFAP-polyclonal-antibody-2539
Chromogenic immunostaining of a formalin fixed paraffin embedded human cerebellum section with rabbit pAb to GFAP, 2539, dilution 1:5,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. The 2539 antibody labels the processes of astrocytes within both the granular and molecular layers and Bergmann glia in the molecular layer. This antibody performs well in testing with both 4% PFA and standard NBF fixed rat, mouse, and human tissues.
ICC/IF-image-rabbit-anti-GFAP-polyclonal-antibody-2539
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with rabbit polyclonal antibody to GFAP, 2539, dilution 1:5,000 in red, and chicken polyclonal antibody to vimentin, 5866, dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. The astrocytes containing GFAP only are red, GFAP and Vimentin are golden-yellow. Fibroblastic cells contain only vimentin, so they are green.
IHC-image-rabbit-anti-GFAP-polyclonal-antibody-2539
Above is an image generated in the Lifecanvas Technologies laboratory using 2539 and an appropriate secondary antibody on a 1mm section of rat brain. This was processed using the Lifecanvas SHIELD fixation protocol which employs a novel epoxy crosslinker (Park et al. Nature Biotechnology 2018).
WB-image-rabbit-anti-GFAP-polyclonal-antibody-2539
Western blot analysis of different tissue lysates using rabbit polyclonal antibody to GFAP, 2539, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Strong band at about 50kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

There are 1040 publications in our database for this antibody or clone. Here are the latest 5, for more click below.


pmidtitleauthorscitation
36600259Delayed NLRP3 inflammasome inhibition ameliorates subacute stroke progression in miceBellut M, Bieber M, Kraft P, Weber ANR, Stoll G, Schuhmann MK.J Neuroinflammation. 2023 Jan 4;20(1):4. doi: 10.1186/s12974-022-02674-w.
36596518Specific Cellular Effects of Low Bortezomib Concentrations on Purified Cultures of Schwann Cells, Satellite Glial Cells, Macrophages, and Dorsal Root Ganglion NeuronsZhou Z, Nagayasu K, Shirakawa H, Kaneko S.Biol Pharm Bull. 2023;46(1):102-110. doi: 10.1248/bpb.b22-00668.
36572898Lactate attenuates astrocytic inflammation by inhibiting ubiquitination and degradation of NDRG2 under oxygen-glucose deprivation conditionsXu J, Ji T, Li G, Zhang H, Zheng Y, Li M, Ma J, Li Y, Chi G.J Neuroinflammation. 2022 Dec 26;19(1):314. doi: 10.1186/s12974-022-02678-6.
36531208Inhibition of Prostaglandin E2 Receptor EP3 Attenuates Oxidative Stress and Neuronal Apoptosis Partially by Modulating p38MAPK/FOXO3/Mul1/Mfn2 Pathway after Subarachnoid Hemorrhage in RatsLiu Y, Liu R, Huang L, Zuo G, Dai J, Gao L, Shi H, Fang Y, Lu Q, Okada T, Wang Z, Hu X, Lenahan C, Tang J, Xiao J, Zhang JH.Oxid Med Cell Longev. 2022 Dec 9;2022:7727616. doi: 10.1155/2022/7727616. eCollection 2022.
36529767The impairment of intramural periarterial drainage in brain after subarachnoid hemorrhageSun Y, Liu E, Pei Y, Yao Q, Ma H, Mu Y, Wang Y, Zhang Y, Yang X, Wang X, Xue J, Zhai J, Carare RO, Qin L, Yan J.Acta Neuropathol Commun. 2022 Dec 18;10(1):187. doi: 10.1186/s40478-022-01492-8.

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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100 reviews for rabbit anti-GFAP polyclonal antibody 2539

  1. verified user (store manager)

    Review details
    Immunohistochemistry free floating
    Sample type:Rat Tissue sections (Adult rat brainstem tissue)
  2. verified user (store manager)

    Review details
    Immunohistochemistry free floating
    Sample type:Rat Tissue sections (Brain)
  3. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Mouse Tissue sections (Brain)
  4. verified user (store manager)

    Review details
    Immunohistochemistry free floating, Rat Tissue sections (Brain
    Sample type:astrocytes)
  5. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Zebra finch Tissue sections (brain)
  6. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Mouse Tissue sections (nerve)
  7. verified user (store manager)

    Review details
    Immunocytochemistry
    Sample type:Mouse Cultured Cells (Neuro2a Cell line)
  8. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Mouse Tissue sections (Brain)
  9. verified user (store manager)

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Mouse Cell (hippocampus)
  10. verified user (store manager)

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    Immunocytochemistry/ Immunofluorescence
    Sample type:Mouse Cell (embryonic intestinal cells)
  11. verified user (store manager)

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Human Cell (HNGC-2)
  12. verified user (store manager)

    Review details
    Immunocytochemistry
    Sample type:Human Cultured Cells (Astrocytes)
  13. verified user (store manager)

    Review details
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample type:Mouse Tissue sections (Brain)
  14. verified user (store manager)

    Review details
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample type:Mouse Tissue sections (Brain)
  15. verified user (store manager)

    Review details
    IHC – Wholemount
    Sample type:Rat Tissue (retina whole mount)
  16. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Mouse Tissue sections (Mouse eye)
  17. verified user (store manager)

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Human Tissue sections (brain)
  18. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Penguin Tissue sections (brain)
  19. verified user (store manager)

    Review details
    Immunocytochemistry
    Sample type:Rat Cultured Cells (33B)
  20. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Rat Tissue sections (spinal cord)
  21. verified user (store manager)

    Review details
    Western blot
    Sample type:Guinea pig Tissue lysate – whole (Hippocampus)
  22. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Mouse Tissue sections (Mouse, brain tissue
    Sample type:whole brain sections)
  23. verified user (store manager)

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Rat Cell (Neural culture)
  24. verified user (store manager)

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Rat Tissue sections (nerve)
  25. verified user (store manager)

    Review details
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample type:Finch (Fringillidae) Tissue sections (brain)
  26. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Dog Tissue sections (brain)
  27. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Dog Tissue sections (Brain)
  28. verified user (store manager)

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Mouse Cell (ITO (Hepatic stellate cells))
  29. verified user (store manager)

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Mouse Tissue sections (E14.5 otic epithelium)
  30. verified user (store manager)

    Review details
    Western blot
    Sample type:Mouse Cell lysate – whole cell (Primary Neural cells)
  31. verified user (store manager)

    Review details
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample type:Monkey Tissue sections (Brain)
  32. verified user (store manager)

    Review details
    Western blot
    Sample type:Mouse Tissue lysate – whole (Whole brain tissue lysate)
  33. verified user (store manager)

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Rat Cell (cortical astrocytes)
  34. verified user (store manager)

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Rat Tissue sections (Spinal Cord)
  35. verified user (store manager)

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Rat Tissue sections (spinal cord)
  36. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Rat Tissue sections (Brain)
  37. verified user (store manager)

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Mouse Cell (Primary cultured brain cell)
  38. verified user (store manager)

    Review details
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample type:Mouse Tissue sections (brain)
  39. verified user (store manager)

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Mouse Cultured Cells (Neural stem cells)
  40. verified user (store manager)

    Review details
    Immunocytochemistry
    Sample type:Human Cultured Cells (HEK)
  41. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Mouse Tissue sections (Mouse brain)
  42. verified user (store manager)

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Human Cell (differentiated iPS cell)
  43. verified user (store manager)

    Review details
    Western blot
    Sample type:Rat Tissue lysate – whole (Rat spinal cord homogenate)
  44. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Rat Tissue sections (Brain cortex)
  45. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Mouse Tissue sections (Brain)
  46. verified user (store manager)

    Review details
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample type:Rat Tissue sections (brain)
  47. verified user (store manager)

    Review details
    Immunocytochemistry
    Sample type:Human Cultured Cells (U87 Human primary Glioblastoma cell line)
  48. verified user (store manager)

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Mouse Cell (Neuro2a cell line)
  49. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Rat Tissue sections (Brain)
  50. verified user (store manager)

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Mouse Cell (brain)
  51. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Rat Tissue sections (Rat brain sections)
  52. verified user (store manager)

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Mouse Tissue sections (Brain (cortex))
  53. verified user (store manager)

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Mouse Tissue sections (Brain – Hippocampus)
  54. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Rat Tissue sections (brain)
  55. verified user (store manager)

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    Western blot
    Sample type:Human Tissue lysate – whole (Brain Lysates)
  56. verified user (store manager)

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    Immunocytochemistry
    Sample type:Human Cultured Cells (Astrocytes)
  57. verified user (store manager)

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    Western blot
    Sample type:Mouse Cell lysate – whole cell (mouse tissues)
  58. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Monkey Tissue sections (Brain)
  59. verified user (store manager)

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Rat Cell (brain)
  60. verified user (store manager)

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    Immunocytochemistry
    Sample type:Mouse Cell (brain)
  61. verified user (store manager)

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    Immunocytochemistry/ Immunofluorescence
    Sample type:Rat Cell (B50 Neuroblastoma cell line)
  62. verified user (store manager)

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Rat Tissue sections (Brain)
  63. verified user (store manager)

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Human Tissue sections (Brain (cortex))
  64. verified user (store manager)

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    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample type:Rat Tissue sections (sciatic nerve)
  65. verified user (store manager)

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Mouse Tissue sections (Brain)
  66. verified user (store manager)

    Review details
    Western blot
    Sample type:Rat Cell lysate – whole cell (B50 Cell line)
  67. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Cat Tissue sections (Cerebellum)
  68. verified user (store manager)

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    Immunocytochemistry/ Immunofluorescence
    Sample type:Human Cell (SH-SY5Y)
  69. verified user (store manager)

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    Western blot
    Sample type:Zebrafish Tissue lysate – whole (Whole fish lysate)
  70. verified user (store manager)

    Review details
    Immunohistochemistry (Frozen sections), Mouse Tissue sections (liver tissue
    Sample type:cryosection)
  71. verified user (store manager)

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Mouse Cell (Cerebellum)
  72. verified user (store manager)

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    Immunohistochemistry (Frozen sections), Human Tissue sections (WM
    Sample type:cortex)
  73. verified user (store manager)

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Mouse Tissue sections (Brain)
  74. verified user (store manager)

    Review details
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample type:Meriones unguiculatus Tissue sections (Neocortex)
  75. verified user (store manager)

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    Western blot
    Sample type:Mouse Cell lysate – whole cell (Neuro2A- neuroblastoma)
  76. verified user (store manager)

    Review details
    Western blot
    Sample type:Human Cell lysate – whole cell (Glioblastoma primary cells)
  77. verified user (store manager)

    Review details
    Immunocytochemistry/ Immunofluorescence, Rat Cell (Rat cerebellum
    Sample type:primary cells)
  78. verified user (store manager)

    Review details
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample type:Mouse Tissue sections (brain)
  79. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Rat Tissue sections (10 micon thick spinal cord sections)
  80. verified user (store manager)

    Review details
    Western blot
    Sample type:Mouse Cell lysate – whole cell (Neuro2a)
  81. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Mouse Tissue sections (spinal cord. brain.)
  82. verified user (store manager)

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Mouse Tissue sections (Brain)
  83. verified user (store manager)

    Review details
    Western blot
    Sample type:Mouse Tissue lysate – whole (Brain)
  84. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Rat Tissue sections (Substantia nigra
    Sample type:astrocytes)
  85. verified user (store manager)

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Mouse Cell (Mouse Tissue sections (Brain))
  86. verified user (store manager)

    Review details
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample type:Mouse Tissue sections (Brain)
  87. verified user (store manager)

    Review details
    Immunocytochemistry
    Sample type:Human Cell (Kelly Neuroblastoma cell line)
  88. verified user (store manager)

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Human Tissue sections (Postmortem brain)
  89. verified user (store manager)

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    Western blot
    Sample type:Rat Tissue lysate – whole (Brain)
  90. verified user (store manager)

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    Immunohistochemistry (Frozen sections)
    Sample type:Human Tissue sections (Retina)
  91. verified user (store manager)

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    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample type:Rat Tissue sections (rat spinal cord)
  92. verified user (store manager)

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    IHC – Wholemount
    Sample type:Mouse Tissue (Intestine)
  93. verified user (store manager)

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Human Tissue sections (large intestine)
  94. verified user (store manager)

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    Immunocytochemistry/ Immunofluorescence
    Sample type:Rat Cell (cortical microglia preparation with astrocytes)
  95. verified user (store manager)

    Review details
    Immunocytochemistry/ Immunofluorescence, Mouse Cell (spinal cord
    Sample type:brain)
  96. verified user (store manager)

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Mouse Tissue sections (large intestine)
  97. verified user (store manager)

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Finch (Fringillidae) Tissue sections (brain)
  98. verified user (store manager)

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    Immunocytochemistry/ Immunofluorescence
    Sample type:Mouse Cell (mouse hippocampus astrocytes)
  99. verified user (store manager)

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Rat Tissue sections (Hippocampus)
  100. verified user (store manager)

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Rat Tissue sections (brain)

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