Immunohistochemistry (IHC) : Human bladder urothelial carcinoma stained with anti-uroplakin antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells.

mouse anti-Uroplakin monoclonal antibody (ZM204) 6400

$160.00 – $528.00

Antibody summary

Mouse monoclonal to Uroplakin
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG2b + IgG1
Control: Bladder or urothelial carcinoma
Visualization: Cytoplasmic and membranous
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6400parent Category: antibody Tags: anatomy, Urinary Pathology, Uroplakin

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2b + IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-Uroplakin monoclonal antibody ZM204 6400

antibody
Database link: human O14601
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Applicaiton Notes Positive control: Bladder or urothelial carcinoma
Immunogen Recombinant fragment (around aa 114-173) of human Uroplakin 1A (UPK1A) protein; recombinant fragment (around aa 109-229) of human Uroplakin 1B (UPK1B)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG2b
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG2b + IgG1 - Isotype C

target relevance
Protein names Uroplakin-1a (UP1a) (Tetraspanin-21) (Tspan-21) (Uroplakin Ia) (UPIa) (UPKa)
Gene names UPK1A,UPK1A TSPAN21
Protein family Tetraspanin (TM4SF) family
Mass 28879Da
Tissues TISSUE SPECIFICITY: High expression restricted to ureteric urothelium (most superficial cells); low expression in prostate. Expression in normal urothelial cells is lost in culture. Some expression in tumor cell lines derived from urothelial malignancies. {ECO:0000269\|PubMed:15913809, ECO:0000269\|PubMed:9846985}.
Structure SUBUNIT: Homodimer; disulfide-linked. Interacts with uroplakin-2 (UPK2) (By similarity). {ECO:0000250}.
Target Relevance information above includes information from UniProt accession: O00322
The UniProt Consortium

Data

Human bladder urothelial carcinoma stained with anti-uroplakin antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.