Immunohistochemistry (IHC) : Human tonsil stained with anti-tryptase antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of scattered mast cells.

mouse anti-Tryptase monoclonal antibody (ZM96) 6439

$160.00 – $528.00

Antibody summary

Mouse monoclonal to Tryptase
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: Mast cells containing tissue or tumors
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6439parent Category: antibody Tags: anatomy, Hematopathology, Tryptase

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-Tryptase monoclonal antibody ZM96 6439

antibody
Database link: human P20231
Tested applications IHC
Recommended dilutions As directed
Immunogen Synthesized peptide corresponding to the C-terminus of human TRPS1 protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names Tryptase beta-2 (Tryptase-2) (EC 3.4.21.59) (Tryptase II)
Protein family Peptidase S1 family, Tryptase subfamily
Mass 30651Da
Function Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
Catalytic activity CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Arg-\|-Xaa, Lys-\|-Xaa, but with more restricted specificity than trypsin.; EC=3.4.21.59;
Subellular location Secreted. Note=Released from the secretory granules upon mast cell activation.
Structure Homotetramer. The active tetramer is converted to inactive monomers at neutral and acidic pH in the absence of heparin. Low concentrations of inactive monomers become active monomers at pH 6.0 in the presence of heparin. When the concentration of active monomers is higher, they convert to active monomers and then to active tetramers. These monomers are active and functionally distinct from the tetrameric enzyme. In contrast to the hidden active sites in the tetrameric form, the active site of the monomeric form is accessible for macromolecular proteins and inhibitors eg: fibrinogen which is a substrate for the monomeric but not for the tetrameric form. The monomeric form forms a complex with SERPINB6.
Domain DOMAIN 31..272; /note="Peptidase S1"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU00274"
Target Relevance information above includes information from UniProt accession: P20231
The UniProt Consortium

Data

Human tonsil stained with anti-tryptase antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of scattered mast cells.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.