Immunohistochemistry (IHC) : Human synovial sarcoma stained with anti-TLE1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells.

mouse anti-TLE1 monoclonal antibody (ZM93) 6387

Price range: $160.00 through $528.00

Antibody summary

Mouse monoclonal to TLE1
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG2a
Control: Synovial sarcoma
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6387parent Category: antibody Tags: anatomy, Soft Tissue Pathology, TLE1

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2a
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-TLE1 monoclonal antibody ZM93 6387

antibody
Database link: human Q04724
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Synovial sarcoma
Immunogen Recombinant human TLE1 fragment (aa 175-338)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG2a
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG2a - Isotype Control

target relevance
Protein names Transducin-like enhancer protein 1 (E(Sp1) homolog) (Enhancer of split groucho-like protein 1) (ESG1)
Gene names TLE1,TLE1
Protein family WD repeat Groucho/TLE family
Mass 83201Da
Function FUNCTION: Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. Enhances FOXG1/BF-1- and HES1-mediated transcriptional repression (By similarity). The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG. {ECO:0000250\|UniProtKB:Q62440, ECO:0000269\|PubMed:10660609}.
Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000269\|PubMed:12397081}. Note=Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components.
Tissues TISSUE SPECIFICITY: In all tissues examined, mostly in brain, liver and muscle.
Structure SUBUNIT: Homooligomer and heterooligomer with other family members. Binds RUNX1, RUNX3, FOXA2, KDM6A, UTY, histone H3, HESX1, ESRRG and the NF-kappa-B subunit RELA. Interacts with HES1 (via WRPW motif). Binds TCF7, LEF1, TCF7L1 and TCF7L2 (By similarity). Interacts with SIX3. Interacts with EFNB1. Interacts with TLE4 (By similarity). Interacts with FOXG1/BF-1; the interaction is inhibited by TLE6/GRG6 (By similarity). {ECO:0000250\|UniProtKB:Q62440, ECO:0000269\|PubMed:10660609, ECO:0000269\|PubMed:10748198, ECO:0000269\|PubMed:11731482, ECO:0000269\|PubMed:12441302, ECO:0000269\|PubMed:14651967, ECO:0000269\|PubMed:21429299, ECO:0000269\|PubMed:8649374, ECO:0000269\|PubMed:8687460, ECO:0000269\|PubMed:9334241, ECO:0000269\|PubMed:9751710, ECO:0000269\|PubMed:9854018, ECO:0000269\|PubMed:9874198}.
Post-translational modification PTM: Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1.; PTM: Ubiquitinated by XIAP/BIRC4. {ECO:0000269\|PubMed:22304967}.
Domain DOMAIN: WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain. {ECO:0000305\|PubMed:18254933}.
Target Relevance information above includes information from UniProt accession: Q04724
The UniProt Consortium

Data

Human synovial sarcoma stained with anti-TLE1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells.

Publications

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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.