Western blot mouse anti p53 monoclonal antibody d01 8319

IHC mouse anti p53 monoclonal antibody d01 8319

mouse anti-p53 monoclonal antibody (D01) 8319

$503.00

Antibody summary

Mouse monoclonal to p53
Suitable for: WB,ICC/IF,IHC-P,IHC-Fr,FACS,IP,ELISA
Isotype: IgG2a
100 µL at 0.2 mg/mL

available sizes

Clear

SKU: 8319parent Categories: antibody, oxidative stress Tag: p53

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2a
clonality	monoclonal
concentration	0.2 mg/mL
applications	ICC/IF, WB
reactivity	p53
available sizes	100 µL

mouse anti-p53 monoclonal antibody (D01) 8319

antibody

Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions IHC (frozen and paraffin), Immunoblotting. IHC: use at 1-10ug/ml. Antigen retrieval: citrate buffer, pH 6.0, 15 minutes. #3339 on paraffin-embedded human breast carcinoma. #3339 on paraffin-embedded human colon carcinoma. Immunoblotting: use at 1-5ug/ml. A band of 53kDa is detected.
Immunogen Recombinant full length protein corresponding to Human p53 (N terminal). epitope is within aa 20-25
Size and concentration 100µg and lot specific
Form liquid
Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer PBS, pH 7.4
Purity protein affinity purification
Clonality monoclonal
Isotype IgG2a
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG2a - Isotype Control

target relevance
Protein names Cellular tumor antigen p53 (Antigen NY-CO-13) (Phosphoprotein p53) (Tumor suppressor p53)
Gene names TP53,TP53 P53
Mass 43653Da

Data

Untreated (-) and treated (-) HCT116 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (8319) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunoprecipitation of p53 protein from 293T whole cell extracts using 5 µg of p53 antibody [DO1] (8319).
Western blot analysis was performed using p53 antibody [D01] (8319).
EasyBlot anti-Mouse IgG \was used as a secondary reagent.

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (8319) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Untreated (-) and treated (-) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (8319) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
p53 stained by p53 antibody [DO1] (8319) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
p53 stained by p53 antibody [DO1] (8319) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

p53 antibody [DO1] detects p53 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
p53 stained by p53 antibody [DO1] (8319) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

293T whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (8319) diluted at 1:40000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Detection of human p53 by western blot. Samples: Whole cell lysate from Hep-G2 (50 µg), A-549 (50 µg), and HEK293T (1 µg) cells prepared using NETN lysis buffer. Antibody: Mouse anti-p53 monoclonal antibody [DO-1] (#8319) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human p53 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Mouse anti-p53 monoclonal antibody [DO-1] (#8319) used for IP at 20 µl/mg lysate. p53 was also immunoprecipitated by a previous lot of this antibody (#8319) and rabbit anti-p53 antibody. For blotting immunoprecipitated p53 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human p53 in FFPE colon carcinoma by immunohistochemsitry. Antibody: Mouse monoclonal anti-p53 [DO-1] (#8319). Secondary:HRP-conjugated goat anti-mouse IgG.Substrate:DAB.

Detection of human p53 in FFPE breast carcinoma by immunohistochemsitry. Antibody: Mouse monoclonal anti-p53 [DO-1] (#8319). Secondary:HRP-conjugated goat anti-mouse IgG.Substrate:DAB.

Detection of human p53 (shaded) in Ramos cells by flow cytometry. Antibody: Mouse anti-p53 recombinant monoclonal [DO-1] (#8319) or isotype control (unshaded). Secondary: DyLight-488-conjugated goat anti-mouse IgG.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.