Immunohistochemistry (IHC) : Human prostate stained with anti-NKX3.1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of glandular cells.

mouse anti-NKX3.1 monoclonal antibody (ZM95) 6291

$160.00 – $528.00

Antibody summary

Mouse monoclonal to NKX3.1
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG2a
Control: Prostate
Visualization: Nuclear
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6291parent Category: antibody Tags: anatomy, NKX3.1, Urinary Pathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2a
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-NKX3.1 monoclonal antibody ZM95 6291

antibody
Database link: human Q99801
Tested applications IHC
Recommended dilutions As directed
Immunogen Human recombinant NKX2.2 protein fragment (aa 1-119)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG2a
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG2a - Isotype Control

target relevance
Protein names Homeobox protein Nkx-3.1 (Homeobox protein NK-3 homolog A)
Protein family NK-3 homeobox family
Mass 26350Da
Function Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. .
Subellular location Nucleus .
Tissues Highly expressed in the prostate and, at a lower level, in the testis. .
Structure Interacts with serum response factor (SRF) (By similarity). Interacts with SPDEF (PubMed:11809674). Interacts with WDR77 (PubMed:12972618). Interacts with TOPORS which polyubiquitinates NKX3-1 and induces its proteasomal degradation (PubMed:18077445). Interacts with FEM1B (By similarity). .
Post-translational modification PTM: Ubiquitinated by TOPORS; monoubiquitinated at several residues and also polyubiquitinated on single residues. .
Target Relevance information above includes information from UniProt accession: Q99801
The UniProt Consortium

Data

Human prostate stained with anti-NKX3.1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of glandular cells.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.