Immunohistochemistry (IHC) : Human diffuse large B cell lymphoma stained with anti-CD45/RB (LCA) antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of lymphoma cells.

mouse anti-CD45RB (LCA) monoclonal antibody (2B11 & PD7-26) 6097

Price range: $160.00 through $528.00

Antibody summary

Mouse monoclonal to CD45RB (LCA)
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1 + IgG1
Control: Tonsil or lymph node
Visualization: Cell membrane
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6097parent Categories: antibody, Zeta IHC antibodies Tags: anatomy, CD45RB (LCA), Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1 + IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-CD45RB (LCA) monoclonal antibody 2B11+PD7&26 6097

antibody
Database link: human P08575
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Tonsil or lymph node
Immunogen PD7/26/16: human peripheral blood lymphocytes maintained in T cell growth factor; 2B11: isolated neoplastic cells from T cell lymphoma
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 + IgG1 - Isotype C

target relevance
Protein names Receptor-type tyrosine-protein phosphatase C (EC 3.1.3.48) (Leukocyte common antigen) (L-CA) (T200) (CD antigen CD45)
Gene names PTPRC,PTPRC CD45
Protein family Protein-tyrosine phosphatase family, Receptor class 1/6 subfamily
Mass 147486Da
Function FUNCTION: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity. {ECO:0000250, ECO:0000269\|PubMed:11909961, ECO:0000269\|PubMed:16998493, ECO:0000269\|PubMed:2845400, ECO:0000269\|PubMed:35767951}.; FUNCTION: (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. {ECO:0000269\|PubMed:22174689}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate; Xref=Rhea:RHEA:10684, Rhea:RHEA-COMP:10136, Rhea:RHEA-COMP:20101, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:46858, ChEBI:CHEBI:61978; EC=3.1.3.48; Evidence={ECO:0000255\|PROSITE-ProRule:PRU10044};
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:12676959, ECO:0000269\|PubMed:22174689}; Single-pass type I membrane protein {ECO:0000255}. Membrane raft {ECO:0000269\|PubMed:12676959}. Synapse {ECO:0000269\|PubMed:35767951}. Note=Colocalized with DPP4 in membrane rafts. {ECO:0000269\|PubMed:12676959}.
Tissues TISSUE SPECIFICITY: Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes. {ECO:0000269\|PubMed:11975983}.
Structure SUBUNIT: Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1 (PubMed:11909961). Interacts with DPP4; the interaction is enhanced in an interleukin-12-dependent manner in activated lymphocytes (PubMed:12676959). Interacts with CD53; this interaction stabilizes PTPRC on the membrane and is required for optimal phosphatase activity (PubMed:35767951). {ECO:0000250\|UniProtKB:P06800, ECO:0000269\|PubMed:11909961, ECO:0000269\|PubMed:12676959, ECO:0000269\|PubMed:35767951}.; SUBUNIT: [Isoform 1]: Interacts with CLEC10A. {ECO:0000269\|PubMed:16998493}.; SUBUNIT: [Isoform 2]: Does not interact with CLEC10A. {ECO:0000269\|PubMed:16998493}.; SUBUNIT: [Isoform 3]: Interacts with CLEC10A. {ECO:0000269\|PubMed:16998493}.; SUBUNIT: [Isoform 5]: Interacts with CLEC10A. {ECO:0000269\|PubMed:16998493}.; SUBUNIT: [Isoform 7]: Interacts with CLEC10A. {ECO:0000269\|PubMed:16998493}.; SUBUNIT: (Microbial infection) Interacts with human cytomegalovirus protein UL11; the interaction is required for binding of UL11 to T-cells. {ECO:0000269\|PubMed:22174689}.
Post-translational modification PTM: Heavily N- and O-glycosylated. {ECO:0000269\|PubMed:19159218, ECO:0000269\|PubMed:19349973}.
Domain DOMAIN: The first PTPase domain interacts with SKAP1.
Involvement in disease DISEASE: Multiple sclerosis (MS) [MIM:126200]: A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheath, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. {ECO:0000269\|PubMed:11101853}. Note=Disease susceptibility may be associated with variants affecting the gene represented in this entry.; DISEASE: Immunodeficiency 105, severe combined (IMD105) [MIM:619924]: An autosomal recessive disorder characterized by recurrent infections in early infancy, decreased or absent numbers of non-functional T cells, normal or increased levels of B cells, hypogammaglobulinemia, and normal or low NK cells. Clinical manifestations may include pneumonia, dermatitis, and lymphadenopathy. {ECO:0000269\|PubMed:10700239, ECO:0000269\|PubMed:11145714, ECO:0000269\|PubMed:22689986}. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P08575
The UniProt Consortium

Data

Human diffuse large B cell lymphoma stained with anti-CD45/RB (LCA) antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of lymphoma cells.

FAQ & Publications

Frequently Asked Questions

What species does the mouse anti-CD45RB (LCA) monoclonal antibody (2B11 & PD7-26) specifically react with?

This antibody specifically reacts with human antigens.

Which applications is this anti-CD45RB antibody validated for?

It is suitable for immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded tissues.

What are the recommended storage conditions for this antibody?

For short-term storage, keep the antibody at 2-8°C. For long-term storage, it should be stored at -20°C, avoiding freeze/thaw cycles.

What is the host species and clonality of this anti-CD45RB antibody?

The antibody is a mouse monoclonal with an IgG1 isotype.

What positive controls are recommended when using this antibody in immunohistochemistry?

Tonsil or lymph node tissues are recommended as positive controls for IHC staining with this antibody.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.