Immunohistochemistry (IHC) : Human reactive lymph node stained with anti-BCL-2 antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining of mantle B-cells and perifollicular T-cells, and no staining of reactive B-cells in the germinal centers.

mouse anti-BCL-2 monoclonal antibody (124) 6029

Price range: $160.00 through $528.00

Antibody summary

Mouse monoclonal to BCL-2
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: Tonsil or lymph node
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

available sizes

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SKU: 6029parent Categories: antibody, Zeta IHC antibodies Tags: anatomy, BCL-2, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-BCL-2 monoclonal antibody 124 6029

antibody
Database link: human P10415
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Tonsil or lymph node
Immunogen A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human bcl-2 protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Homo sapiens BCL2 Apoptosis regulator Bcl-2
Protein names Apoptosis regulator Bcl-2
Gene names BCL2
Protein family Belongs to the Bcl-2 family
Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:20889974, PubMed:21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785)
Subcellular location Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm
Structure (Microbial infection) Interacts with Toxoplasma gondii ROP17; the interaction probably promotes BCL2 phosphorylation and degradation
Post-translational modification Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity (PubMed:11368354). Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle (PubMed:11368354). In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases (PubMed:11368354). Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, which stimulates starvation-induced autophagy (PubMed:10567572, PubMed:18570871). Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity) Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity Monoubiquitinated by PRKN, leading to an increase in its stability (PubMed:20889974). Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome (PubMed:23431138). Ubiquitinated by XIAP, leading to its degradation by the proteasome (PubMed:29020630)
Keywords 3D-structure, Alternative splicing, Apoptosis, Autophagy, Chromosomal rearrangement, Cytoplasm, Disease variant, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus, Phosphoprotein, Proteomics identification, Proto-oncogene, Reference proteome, Transmembrane, Transmembrane helix, Ubl conjugation
Sequence MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPA ASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLH LTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEY LNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLSLALVGACITLGAYLGHK
UniProt accession: P10415

Data

Human reactive lymph node stained with anti-BCL-2 antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining of mantle B-cells and perifollicular T-cells, and no staining of reactive B-cells in the germinal centers.

FAQ & Publications

Frequently Asked Questions

For which species is the mouse anti-BCL-2 monoclonal antibody (124) 6029 reactive?

This antibody is reactive with human tissues.

What applications is this anti-BCL-2 monoclonal antibody validated for?

It is suitable for immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded tissues.

How should the mouse anti-BCL-2 antibody be stored to maintain stability?

Store the antibody at 2-8°C for short-term use, and for longer-term storage, keep it at -20°C while avoiding freeze/thaw cycles.

What are the available sizes and concentrations for this BCL-2 antibody?

The antibody is available as concentrated formats in 0.1 mL, 0.5 mL, and 1.0 mL volumes, as well as a 7 mL prediluted format.

What is the recommended dilution range for using the concentrated mouse anti-BCL-2 monoclonal antibody in IHC?

The recommended dilution for the concentrated antibody is between 1:100 and 1:200.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.