HSV/VZV RT-PCR test Mikrogen 830572

$487.00

Summary

Mikrogen diagnostik RT PCR kit for research use (RUO)
Direct Herpes Simplex Virus (HSV), Varizella Zoster Virus detection
High sensitivity and specificity
Internal control for monitoring nucleic acid extraction
(RNA/DNA) and real-time PCR inhibition in each reaction
Compatible with most common real-time PCR cyclers & RNA/DNA extraction methods
96 tests

SKU: 830572 Category: Mikrogen kits Tags: Herpes simplex Virus, RT PCR, Varicella-Zoster Virus

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	HSV/VZV
species reactivity	Real Time PCR for direct detection of Herpes Simplex Virus (HSV-1, HSV-2) and Varicella Zoster Virus
applications	RT PCR
assay type	direct & qualitative
available sizes	96 tests

HSV/VZV RT-PCR test Mikrogen 830572

Assay type
RT PCR

Research area
Infectious Disease

Sample type
whole blood, serum, plasma, urine, tissue, stool, etc., food and environmental samples or from the carrier material

Notes

Mic (Magnetic Induction Cycler)	validated
Roche LightCycler(c) 480 Instrument II	validated
Roche covas z 480 Analyzer	validated
Qiagen Rotor-Gene(c) Q	validated
Bio-Rad CFX 96	validated
Applied Biosystems (QuantStudio TM 5 Dx)	validated
Stratagene Mx3000P	compatible

Components

Reaction Mix	Cap color - yellow	2 x 768 µl
Positive Control	Cap color - red	1 x 100 µl
Negative Control	Cap color - green	1 x 100 µl
Control DNA	Cap color - colorless	2 x 240 µl
Instructions for Use		1 Each

Storage
Store at -20°C.

Additional information

Highly sensitive and specific direct detection of pathogens that can cause tick-borne infections Applicable to human starting material as well as RNA/DNA from the tick

Complete PCR kits with ready-to-use reagents

Compatible with common real-time PCR cyclers Compatible with various RNA/DNA extraction methods (e.g. Mikrogen alphaClean Mag RNA/DNA Kits)

target relevance

human antibody anti-Herpes Simplex Virus (HSV)

Herpes Simplex Virus (HSV)

Description
Virus that causes oral herpes, genital herpes, and other mucocutaneous infections

Structure
Herpes simplex viruses (HSV) are enveloped, double-stranded DNA viruses belonging to the genus Simplexvirus within the family Herpesviridae. Two closely related species, Herpes simplex virus type 1 (HSV-1) and Herpes simplex virus type 2 (HSV-2), are responsible for the majority of human infections. The viral genome is enclosed within an icosahedral capsid surrounded by a protein tegument and a lipid envelope containing glycoproteins that mediate attachment and entry into host cells. Following primary infection, HSV establishes lifelong latency within sensory neurons, particularly in the trigeminal and sacral ganglia. Periodic reactivation of latent virus results in recurrent infections and viral shedding, often in the absence of symptoms. HSV infections are widespread globally and represent some of the most common viral infections of humans.

Diagnosis
The diagnosis of herpes simplex virus infection is performed through clinical evaluation and laboratory testing. Molecular methods such as polymerase chain reaction (PCR) are highly sensitive and specific and are considered the preferred diagnostic approach for detecting HSV DNA in lesion swabs, cerebrospinal fluid, and other clinical specimens. Viral culture may also be used, particularly for active lesions, although it is generally less sensitive than PCR. Direct fluorescent antibody testing and antigen detection assays may provide rapid identification in selected settings. Serological assays, including ELISA-based tests, can detect HSV-specific antibodies and help distinguish previous exposure to HSV-1 and HSV-2. Laboratory confirmation is particularly important in cases of neonatal herpes, encephalitis, and atypical presentations.

Symptoms
Herpes simplex virus infections produce a wide range of clinical manifestations depending on the site of infection and immune status of the host. Primary infection may be asymptomatic or may present with painful vesicular lesions involving the oral cavity, lips, genital region, skin, or eyes. HSV-1 most commonly causes oral herpes characterized by fever, gingivostomatitis, and recurrent cold sores, whereas HSV-2 is more frequently associated with genital herpes causing painful ulcers, itching, dysuria, and regional lymphadenopathy. Recurrent episodes are typically milder than primary infections due to partial immune control. In immunocompromised individuals and neonates, HSV can cause severe disseminated disease. Rare but serious complications include herpes encephalitis, keratitis leading to vision loss, meningitis, and neonatal herpes, which may be life-threatening if untreated.

Data

No results found

FAQ & Publications

Frequently Asked Questions

What pathogens does the HSV/VZV RT-PCR test Mikrogen 830572 detect?

This RT-PCR test is designed for the direct detection of Herpes Simplex Virus types 1 and 2 (HSV-1, HSV-2) and Varicella Zoster Virus (VZV).

Which sample types are compatible with the HSV/VZV RT-PCR test Mikrogen 830572?

The test can be applied to a wide range of sample types including whole blood, serum, plasma, urine, tissue, stool, as well as food and environmental samples or carrier material.

Is the HSV/VZV RT-PCR test Mikrogen 830572 compatible with different real-time PCR cyclers?

Yes, it is validated for use with several common real-time PCR cyclers including the Roche LightCycler 480 Instrument II, Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Bio-Rad CFX 96, Applied Biosystems QuantStudio 5 Dx, and Stratagene Mx3000P.

How should the HSV/VZV RT-PCR test Mikrogen 830572 be stored to maintain stability?

The kit components should be stored at -20°C to ensure stability and optimal performance.

Does the HSV/VZV RT-PCR test include controls to monitor the PCR process?

Yes, the kit contains internal controls for monitoring nucleic acid extraction and real-time PCR inhibition in each reaction, including positive and negative controls as well as control DNA.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
This product has moved to a digital protocol. Please use the URL provided on the product packaging to access the electronic Instructions for Use (eIFU). 830572 protocol

Documents

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HSV/VZV RT-PCR test Mikrogen 830572

Data

FAQ & Publications

Protocols

Documents

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