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gastroplexVirus PLUS 2 RT-PCR test Mikrogen 830503

$487.00

Summary

  • Mikrogen diagnostik RT PCR kit for research use (RUO)
  • Direct Adenovirus, Norovirus, Rotavirus, Astrovirus, Sapovirus detection
  • High sensitivity and specificity
  • Internal control for monitoring nucleic acid extraction
    (RNA/DNA) and real-time PCR inhibition in each reaction
  • Compatible with most common real-time PCR cyclers & RNA/DNA extraction methods
  • 96 tests
Weight 1 lbs
Dimensions 9 × 5 × 2 in
target

gastroplexVirus PLUS 2

species reactivity

For the detection of Norovirus Genogroup I, Norovirus Genogroup II, Adenovirus, Rotavirus, Astrovirus, and Sapovirus.

applications

RT PCR

assay type

direct & qualitative

available sizes

96 tests

gastroplexVirus PLUS 2 RT-PCR test Mikrogen 830503

kit
Assay type
RT PCR
Research area
Infectious Disease
Sample type
whole blood, serum, plasma, urine, tissue, stool, etc., food and environmental samples or from the carrier material
Notes
Mic (Magnetic Induction Cycler) validated
Roche LightCycler(c) 480 Instrument II validated
Roche covas z 480 Analyzer validated
Qiagen Rotor-Gene(c) Q validated
Bio-Rad CFX 96 validated
Applied Biosystems (QuantStudio TM 5 Dx) validated
Stratagene Mx3000P compatible
Components
Reaction Mix Cap color - yellow 2 x 768 µl
Positive Control Cap color - red 1 x 100 µl
Negative Control Cap color - green 1 x 100 µl
Control DNA Cap color - colorless 2 x 240 µl
Instructions for Use 1 Each
Storage
Store at -20°C.
Additional information

Highly sensitive and specific direct detection of pathogens that can cause tick-borne infections Applicable to human starting material as well as RNA/DNA from the tick

Complete PCR kits with ready-to-use reagents

Compatible with common real-time PCR cyclers Compatible with various RNA/DNA extraction methods (e.g. Mikrogen alphaClean Mag RNA/DNA Kits)

target relevance
Adenovirus
Description
Virus that causes respiratory, ocular, and gastrointestinal infections
Structure
Adenoviruses are non-enveloped, double-stranded DNA viruses belonging to the family Adenoviridae. The viral genome is enclosed within an icosahedral capsid composed of hexon and penton proteins, with characteristic fiber proteins projecting from the vertices that mediate attachment to host cells. Adenoviruses are highly stable in the environment and resistant to many physical and chemical agents, allowing transmission through respiratory droplets, direct contact, contaminated surfaces, and fecal-oral routes. More than 100 adenovirus types have been identified, with numerous species capable of infecting humans. Adenoviruses primarily infect epithelial cells of the respiratory tract, gastrointestinal tract, urinary tract, and conjunctiva, and certain serotypes can establish persistent infections in lymphoid tissues.
Diagnosis
The diagnosis of adenovirus infection is performed through the detection of viral DNA, viral antigens, or adenovirus-specific antibodies. Polymerase chain reaction (PCR) assays are the most sensitive and specific diagnostic methods and are widely used for the detection and typing of adenoviruses from respiratory secretions, stool samples, conjunctival swabs, blood, and other clinical specimens. Antigen detection assays and viral culture may also be used in selected clinical settings. Serological methods, including ELISA, can be employed to detect adenovirus-specific antibodies and assess previous exposure or immune responses. Laboratory confirmation is particularly important in immunocompromised patients, transplant recipients, and individuals with severe or disseminated disease.
Symptoms
Adenovirus infections can produce a broad range of clinical manifestations depending on the infecting serotype and the affected organ system. Respiratory infections commonly present with fever, sore throat, cough, nasal congestion, and pharyngitis and may progress to bronchitis or pneumonia. Ocular infections can cause conjunctivitis or epidemic keratoconjunctivitis characterized by eye redness, irritation, tearing, and photophobia. Gastrointestinal infections may result in diarrhea, vomiting, abdominal pain, and fever, particularly in young children. Although most infections are self-limiting, severe disease may occur in infants, elderly individuals, and immunocompromised patients, where adenovirus can cause disseminated infection involving multiple organ systems.

Data

No results found

FAQ & Publications

Frequently Asked Questions
Which viral pathogens does the gastroplexVirus PLUS 2 RT-PCR test detect?
The gastroplexVirus PLUS 2 RT-PCR test detects Norovirus Genogroup I, Norovirus Genogroup II, Adenovirus, Rotavirus, Astrovirus, and Sapovirus.
Is the gastroplexVirus PLUS 2 RT-PCR test compatible with common real-time PCR cyclers and nucleic acid extraction methods?
Yes, the test is compatible with most common real-time PCR cyclers including Roche LightCycler 480 Instrument II, Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Bio-Rad CFX 96, Applied Biosystems QuantStudio 5 Dx, and Stratagene Mx3000P. It is also compatible with various RNA/DNA extraction methods such as Mikrogen alphaClean Mag RNA/DNA Kits.
Publications
pmid title authors citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
This product has moved to a digital protocol. Please use the URL provided on the product packaging to access the electronic Instructions for Use (eIFU).
830503 protocol

Documents

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