FLAG-Tag (DYKDDDDK-Tag Protein) ELISA Kit 60811

$570.00

Summary

ELISA kit for research use (RUO)
Reactivity: DYKDDDDK-tag (FLAG)
Suitable for: ELISA
Time:3 hours
Sample types: Serum, plasma, tissue homogenates and other biological fluids

SKU: 60811 Category: cELISA Tags: FLAG-Tag (DYKDDDDK-Tag Protein), HCP, sandwich (sELISA)

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	FLAG-Tag (DYKDDDDK-Tag Protein)
species reactivity	universal
applications	competition (cELISA)
assay type	direct & quantitative
available size	96 tests
sensitivity	9.375 ng/ml
range	15.625–1000 ng/ml

FLAG-Tag (DYKDDDDK-Tag Protein) ELISA Kit

Assay type
competition (cELISA)

Research area
host cell protein

Sample type
Serum, plasma, tissue homogenates and other biological fluids

Components

Break apart microtiter test strips each coated single wells	8 x 12 (96 Total)
Lyophilized Standard	2 x vial
Biotin-labeled Antibody(Concentrated, 100X)	60 uL
HRP-Streptavidin Conjugate(Concentrated, 100X)	120 uL
Washing solution concentrate (25X)	30 mL
Sample Dilution buffer	20 mL
Antibody Dilution buffer	10 mL
Streptavidin Dilution buffer	10 mL
Stopping solution	10 mL
TMB Substrate (ready-to-use)	10 mL
Plate seals	3

Storage
Store at 2-8°C.

target relevance
DDDDK
Protein names FLAG tag
Alternative names FLAG tag, FLAG epitope, DYKDDDDK tag, DYDDDDK tag, FLAG peptide
Function The FLAG tag serves as an affinity and detection tag that can be fused to recombinant proteins. It is recognized by anti-FLAG antibodies and affinity reagents for purification, immunoprecipitation, western blotting, immunofluorescence, flow cytometry, and ELISA.
Description The FLAG tag is a short artificial peptide epitope widely used in recombinant protein expression, purification, detection, and localization studies. The canonical FLAG sequence is DYKDDDDK.
Biological role Recombinant protein detection Affinity purification Immunoprecipitation Protein localization studies
Research relevance FLAG-tagged proteins are extensively used in molecular biology and protein expression systems because the tag is small, highly immunogenic, and generally does not interfere with protein folding or function.
Keywords Epitope tag, Protein purification, Protein detection, Recombinant protein
Structure The FLAG tag is a synthetic peptide epitope with the canonical amino acid sequence DYKDDDDK.
Function FLAG serves as a molecular tag for detection, purification, and characterization of recombinant proteins.
Detection FLAG-tagged proteins are commonly detected using anti-FLAG antibodies such as M1 and M2 clones in western blotting, ELISA, immunofluorescence, and immunoprecipitation assays.
Purification FLAG affinity resins and magnetic beads enable rapid purification of FLAG-tagged proteins under native or denaturing conditions.
Research relevance The FLAG tag is one of the most widely used epitope tags in molecular biology, biotechnology, and recombinant protein production.

Data

Standard Curve

X axis: log | Y axis: linear

Standard	STD [ng/mL]	OD1	OD2	Average OD	Corrected OD
Blank #8	0	2.28	2.39	2.34	0
Standard #7	15.63	1.84	1.93	1.88	-0.45
Standard #6	31.25	1.65	1.73	1.69	-0.65
Standard #5	62.5	1.58	1.66	1.62	-0.72
Standard #4	125	1.26	1.32	1.29	-1.05
Standard #3	250	1.01	1.07	1.04	-1.3
Standard #2	500	0.76	0.8	0.78	-1.56
Standard #1	1000	0.61	0.64	0.63	-1.71

Precision

Sample	n	Mean	Standard Deviation	CV (%)
Intra Sample 1	20	30.63	1.38	4.51
Inter Sample 1	20	31.16	1.4	4.48
Intra Sample 2	20	121.9	6.02	4.94
Inter Sample 2	20	125.3	6.01	4.8
Intra Sample 3	20	518	25.23	4.87
Inter Sample 3	20	523	23.22	4.44

Recovery

Matrix	Recovery Range (%)	Average (%)
Cell Culture Lysate(n=5)	85-105	95

Stability

37°C 1 Month (%)	2–8°C 6 Months (%)	2–8°C 12 Months (%)
80	95-100	85-98

Linearity

Sample	1:2 Dilution (%)	1:4 Dilution (%)	1:8 Dilution (%)
Cell Culture Lysate(n=5)	82-98%	84-101%	90-98%

FAQ & Publications

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.