E. coli HCPs (E. coli host cell proteins) ELISA Kit 60808

$570.00

Summary

ELISA kit for research use (RUO)
Reactivity: E.coli proteins
Suitable for: ELISA
Time:3 hours
Sample types: Serum, plasma, tissue homogenates and other biological fluids

SKU: 60808 Category: sELISA Tags: E. coli HCPs (E. coli host cell proteins), HCP, sandwich (sELISA)

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	E. coli HCPs (E. coli host cell proteins)
species reactivity	E.coli
applications	sandwich (sELISA)
assay type	direct & quantitative
available size	96 tests
sensitivity	1.875 ng/ml
range	3.125–200 ng/ml

E. coli HCPs (E. coli host cell proteins) ELISA Kit

Assay type
sandwich (sELISA)

Research area
host cell protein

Sample type
Serum, plasma, tissue homogenates and other biological fluids

Components

Break apart microtiter test strips each coated single wells	8 x 12 (96 Total)
Lyophilized Standard	2 x vial
Biotin-labeled Antibody(Concentrated, 100X)	120 uL
HRP-Streptavidin Conjugate(Concentrated, 100X)	120 uL
Washing solution concentrate (25X)	30 mL
Sample Dilution buffer	20 mL
Antibody Dilution buffer	10 mL
Streptavidin Dilution buffer	10 mL
Stopping solution	10 mL
TMB Substrate (ready-to-use)	10 mL
Plate seals	3

Storage
Store at 2-8°C.

target relevance
ECOLI host cell proteins
Protein names Escherichia coli host cell proteins
Alternative names E. coli HCP, ECOLI HCP, Host cell proteins from Escherichia coli
Description Residual proteins derived from Escherichia coli expression systems used in recombinant protein production.

Data

Standard Curve

X axis: log | Y axis: linear

Standard	STD [ng/mL]	OD1	OD2	Average OD	Corrected OD
Blank #8	0	0.1	0.11	0.1	0
Standard #7	3.13	0.18	0.18	0.18	0.07
Standard #6	6.25	0.26	0.26	0.26	0.16
Standard #5	12.5	0.35	0.36	0.36	0.25
Standard #4	25	0.63	0.65	0.64	0.54
Standard #3	50	0.99	1.02	1	0.9
Standard #2	100	1.66	1.71	1.68	1.58
Standard #1	200	2.27	2.34	2.31	2.2

Precision

Sample	n	Mean	Standard Deviation	CV (%)
Intra Sample 1	20	6.19	0.33	5.32
Inter Sample 1	20	6.01	0.24	3.94
Intra Sample 2	20	25.52	1.25	4.88
Inter Sample 2	20	25.91	1.08	4.16
Intra Sample 3	20	98.53	3.85	3.91
Inter Sample 3	20	98.31	4.16	4.23

Recovery

Matrix	Recovery Range (%)	Average (%)
Cell lysate(n=10)	90-105	95

Stability

37°C 1 Month (%)	2–8°C 6 Months (%)	2–8°C 12 Months (%)
80	95-100	85-98

FAQ & Publications

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.