LPS (Lipopolysaccharides) ELISA Kit 60804

$570.00

Summary

ELISA kit for research use (RUO)
Reactivity: LPS (Lipopolysaccharides)
Suitable for: ELISA
Time:3 hours
Sample types: Serum, plasma, tissue homogenates and other biological fluids

SKU: 60804 Category: cELISA Tags: Biotin, HCP, LPS (Lipopolysaccharides)

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	LPS (Lipopolysaccharides)
species reactivity	universal
applications	competition (cELISA)
assay type	direct & quantitative
available size	96 tests
sensitivity	0.188 µg/ml
range	0.313–20 µg/ml

LPS (Lipopolysaccharides) ELISA Kit

Assay type
competition (cELISA)

Research area
host cell protein

Sample type
Serum, plasma, tissue homogenates and other biological fluids

Components

Break apart microtiter test strips each coated single wells	8 x 12 (96 Total)
Lyophilized Standard	2 x vial
Biotin-labeled Antibody(Concentrated, 100X)	120 uL
HRP-Streptavidin Conjugate(Concentrated, 100X)	120 uL
Washing solution concentrate (25X)	30 mL
Sample Dilution buffer	20 mL
Antibody Dilution buffer	10 mL
Streptavidin Dilution buffer	10 mL
Stopping solution	10 mL
TMB Substrate (ready-to-use)	10 mL
Plate seals	3

Storage
Store at 2-8°C.

target relevance
LPS (Lipopolysaccharides)
Protein names Lipopolysaccharide
Alternative names LPS, Endotoxin, Bacterial endotoxin, Lipopolysaccharides
Function LPS activates immune cells primarily through the TLR4 signaling pathway.
Description Lipopolysaccharide is a major component of the outer membrane of Gram-negative bacteria and is a potent activator of innate immune responses.

Data

Standard Curve

X axis: log | Y axis: linear

Standard	STD [ng/mL]	OD1	OD2	Average OD	Corrected OD
Blank #8	0	2.33	2.45	2.39	0
Standard #7	0.31	1.73	1.82	1.78	-0.62
Standard #6	0.63	1.44	1.51	1.47	-0.92
Standard #5	1.25	1.27	1.33	1.3	-1.1
Standard #4	2.5	1.07	1.13	1.1	-1.29
Standard #3	5	0.83	0.87	0.85	-1.55
Standard #2	10	0.72	0.75	0.73	-1.66
Standard #1	20	0.68	0.71	0.69	-1.7

Precision

Sample	n	Mean	Standard Deviation	CV (%)
Intra Sample 1	20	0.62	0.03	4.68
Inter Sample 1	20	0.62	0.03	5.23
Intra Sample 2	20	2.6	0.13	4.88
Inter Sample 2	20	2.51	0.12	4.96
Intra Sample 3	20	10.38	0.34	3.28
Inter Sample 3	20	10.06	0.63	6.24

Recovery

Matrix	Recovery Range (%)	Average (%)
bacteria culture medium(n=5)	85-101	97

Stability

37°C 1 Month (%)	2–8°C 6 Months (%)	2–8°C 12 Months (%)
80	95-100	85-98

Linearity

Sample	1:2 Dilution (%)	1:4 Dilution (%)	1:8 Dilution (%)
bacteria culture medium (n=5)	85-103%	88-94%	86-104%

FAQ & Publications

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.