Bordetella RT-PCR test Mikrogen 830527

$487.00

Summary

Mikrogen diagnostik RT PCR kit for research use (RUO)
Direct Bordetella pertussis, Bordetella parapertussis detection
High sensitivity and specificity
Internal control for monitoring nucleic acid extraction
(RNA/DNA) and real-time PCR inhibition in each reaction
Compatible with most common real-time PCR cyclers & RNA/DNA extraction methods
96 tests

SKU: 830527 Category: Mikrogen kits Tags: Bordetella pertussis, RT PCR

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	Bordetella
species reactivity	For the detection of Bordetella pertussis and Bordetella parapertussis
applications	RT PCR
assay type	direct & qualitative
available sizes	96 tests

Bordetella RT-PCR test Mikrogen 830527

Assay type
RT PCR

Research area
Infectious Disease

Sample type
whole blood, serum, plasma, urine, tissue, stool, etc., food and environmental samples or from the carrier material

Notes

Mic (Magnetic Induction Cycler)	validated
Roche LightCycler 480 Instrument II	validated
Roche covas z 480 Analyzer	validated
Qiagen Rotor-Gene(c) Q	validated
Bio-Rad CFX 96	validated
Applied Biosystems (QuantStudio TM 5 Dx)	validated
Stratagene Mx3000P	compatible

Components

Reaction Mix	Cap color - yellow	2 x 768 µl
Positive Control	Cap color - red	1 x 100 µl
Negative Control	Cap color - green	1 x 100 µl
Control DNA	Cap color - colorless	2 x 240 µl
Instructions for Use		1 Each

Storage
Store at -20°C.

Additional information

Highly sensitive and specific direct detection of pathogens that can cause tick-borne infections Applicable to human starting material as well as RNA/DNA from the tick

Complete PCR kits with ready-to-use reagents

Compatible with common real-time PCR cyclers Compatible with various RNA/DNA extraction methods (e.g. Mikrogen alphaClean Mag RNA/DNA Kits)

target relevance

human antibody anti-Bordetella pertussis

Bordetella pertussis

Description
Bacterium that causes whooping cough (pertussis)

Structure
Bordetella pertussis is a small, Gram-negative, aerobic coccobacillus belonging to the family Alcaligenaceae. The bacterium is a strictly human pathogen and the primary causative agent of pertussis, commonly known as whooping cough. B. pertussis colonizes the ciliated epithelium of the upper respiratory tract through the action of multiple adhesins, including filamentous hemagglutinin, pertactin, and fimbriae. The organism produces several virulence factors, most notably pertussis toxin, adenylate cyclase toxin, and tracheal cytotoxin, which interfere with host immune responses and contribute to respiratory tract damage. Despite widespread vaccination programs, Bordetella pertussis remains a significant cause of respiratory disease worldwide, particularly among infants and inadequately vaccinated populations.

Diagnosis
The diagnosis of Bordetella pertussis infection is performed using microbiological, molecular, and serological methods. Polymerase chain reaction (PCR) testing of nasopharyngeal specimens is the preferred laboratory method during the early stages of infection due to its high sensitivity and rapid turnaround time. Culture of B. pertussis from nasopharyngeal swabs remains the definitive diagnostic method but is less sensitive and requires specialized media and prolonged incubation. Serological assays, including ELISA-based tests that detect antibodies against pertussis toxin and other bacterial antigens, are useful in later stages of disease when bacterial shedding has decreased. Laboratory diagnosis is particularly important because clinical presentation may overlap with other respiratory infections.

Symptoms
Bordetella pertussis infection is highly contagious and spreads through respiratory droplets generated during coughing or sneezing. Following an incubation period of approximately seven to ten days, disease typically progresses through three stages. The catarrhal stage resembles a common upper respiratory infection with symptoms including runny nose, mild fever, sneezing, and occasional cough. This is followed by the paroxysmal stage, characterized by severe repetitive coughing fits that may be followed by the characteristic inspiratory 'whoop,' post-tussive vomiting, and exhaustion. In infants, apnea may occur and can be life-threatening. The final convalescent stage involves gradual recovery over weeks to months, although coughing episodes may persist. Complications include pneumonia, seizures, encephalopathy, rib fractures, and, particularly in young infants, death.

Data

RT PCR specificity [830527]

Immunofluorescent analysis of of SH-SY5Y cells stained with mouse mAb to calreticulin, MCA-6C6, dilution 1:500 in green and costained with chicken pAb to lamin A/C, CPCA-LaminAC, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. The MCA-6C6 antibody reveals granular staining of cytoplasm, while the lamin A/C antibody stains the nuclear lamina and membrane.

FAQ & Publications

Frequently Asked Questions

What Bordetella species does the Mikrogen 830527 RT-PCR test detect?

The Mikrogen 830527 RT-PCR test is designed for the direct detection of Bordetella pertussis and Bordetella parapertussis species.

Which real-time PCR cyclers have been validated for use with this Bordetella RT-PCR kit?

The kit has been validated for use with the Roche LightCycler 480 Instrument II, Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Bio-Rad CFX 96, Applied Biosystems QuantStudio 5 Dx, and Stratagene Mx3000P.

What types of samples are compatible with the Bordetella RT-PCR test Mikrogen 830527?

This test is compatible with a variety of sample types including whole blood, serum, plasma, urine, tissue, stool, food, environmental samples, and carrier material.

How should the Bordetella RT-PCR test kit be stored to maintain reagent stability?

The kit components should be stored at -20°C to ensure stability and optimal performance.

Does the Mikrogen Bordetella RT-PCR kit include controls for assay validation?

Yes, the kit includes a positive control, negative control, and control DNA to monitor nucleic acid extraction and PCR inhibition in each reaction.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
This product has moved to a digital protocol. Please use the URL provided on the product packaging to access the electronic Instructions for Use (eIFU). 830527 protocol

Documents

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Bordetella RT-PCR test Mikrogen 830527

Data

FAQ & Publications

Protocols

Documents

Reviews

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