| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| target | Coronavirus |
| species reactivity | For detection of Middle East Respiratory Syndrome Coronavirus (MERS CoV, SARS coronaviruses (incl. SARS-CoV-2 / COVID 19), human coronaviruses (NL63, OC43, 229E, HKU). |
| applications | RT PCR |
| assay type | direct & qualitative |
| available sizes | 96 tests |
Coronavirus Panel RT-PCR test Mikrogen 50142
$487.00
Summary
- Mikrogen diagnostik RT PCR kit for research use (RUO)
- Direct SARS-CoV-2, Coronavirus, MERS-CoV detection
- High sensitivity and specificity
- Internal control for monitoring nucleic acid extraction
(RNA/DNA) and real-time PCR inhibition in each reaction - Compatible with most common real-time PCR cyclers & RNA/DNA extraction methods
- 96 tests
Coronavirus Panel RT-PCR test Mikrogen 50142
| kit | |||||||||||||||
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| Assay type RT PCR | |||||||||||||||
| Research area Infectious Disease | |||||||||||||||
| Sample type whole blood, serum, plasma, urine, tissue, stool, etc., food and environmental samples or from the carrier material | |||||||||||||||
Notes
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Components
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| Storage Store at -20°C. | |||||||||||||||
| Additional information Highly sensitive and specific direct detection of pathogens that can cause tick-borne infections
Applicable to human starting material as well as RNA/DNA from the tick |
| target relevance |
|---|
| Organism SARS-CoV-2 |
| Structure and strains SARS-CoV-2 consists of 4 structural proteins and 16 non-structural proteins. The 4 structural proteins are spike (S), envelope (E), nucleocapsid (N) and membrane (M) protein. The N protein is associated with the RNA genome, the S, E and M proteins together form the virus envelope. The S protein is a glycoprotein consisting of two domains, the S1 domain, which contains the receptor binding domain (RBD) responsible for the virus being able to attach to and fuse with the ACE2 receptor of the host cell, and the S2 domain including transmembrane and endodomain. On the virus surface, the S protein forms trimeric structures. N and S protein are major immunogenic proteins of the virus family Coronaviridae and are very well suited for serological detection of anti-SARS-CoV-2 antibodies. Recombinant versions of both proteins are already widely used in different test systems. |
| Disease Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a strain of coronavirus that causes COVID-19, the respiratory illness responsible for the COVID-19 pandemic. The virus previously had the provisional name 2019 novel coronavirus (2019-nCoV),and has also been called human coronavirus 2019 (HCoV-19 or hCoV-19). SARS-CoV-2 is a positive-sense single-stranded RNA virus that is contagious in humans. |
| Detection and diagnosis Neutralizing antibodies during host immune responses are predominantly directed against S protein, which is supposedly suitable for very specific test settings as it is less conserved within the family of Coronaviridae than the other immunogenic antigens. However, recent studies have shown that antibodies against N protein are detectable earlier in mild infections than antibodies directed against S protein. As an acute marker in particular, it can be beneficial to use N protein for most sensitive early detection. In the further course of the immune response, the N protein antibody level decreases, whereas antibodies against S protein are detectable for a longer time. Various studies show that anti-SARS-CoV-2 antibodies of the IgA and IgM class are detectable in the median at the earliest from about six days after the onset of symptoms, increase in the further course of the disease and decrease again between 18 and 35 days. Antibodies of the IgG class are detectable in the median ten to eighteen days after onset of symptoms and are detectable over several months. |
Data
| No results found |
Publications
Publications
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| We haven't added any publications to our database yet. | |||
Protocols
| relevant to this product |
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| 50142 protocol |
Documents
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