| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| available sizes | 1 mg, 100 µg, 25 µg |
rabbit anti-DYDDDDK tag polyclonal antibody 4849
$100.00 – $2,600.00
Antibody summary
- Rabbit polyclonal to DYDDDDK tag
- Suitable for: WB, ICC/IF, ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgG
- 100 µg, 25 µg, 1 mg
rabbit anti-DYDDDDK tag polyclonal antibody 4849
| antibody |
|---|
| Database link: DYDDDDK tag (FLAG tag) |
| Tested applications WB,ICC/IF,ELISA |
| Recommended dilutions user optimized |
| Immunogen Highly purified DYKDDDDK epitope conjugated to KLH |
| Size and concentration 25, 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions 2-8°C |
| Storage buffer PBS, pH 7.2, 0.09% NaN3 |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| DYDDDDK (Flag) tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify DYDDDDK (Flag) tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, DYDDDDK (Flag) tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody. Click for more on: epitope tags and DYDDDDK (Flag) tag |
| Biotechnology The DYDDDDK tag, also known as the FLAG tag, is a short peptide sequence that has become a popular and powerful tool in recombinant protein expression. When fused to the N- or C-terminus of a target protein, the DYDDDDK tag allows for easy detection, purification, and immunoprecipitation of the expressed protein. Its small size and minimal impact on protein folding and function make it an attractive choice for various research applications. The DYDDDDK tag is recognized by commercially available high-affinity antibodies, facilitating the sensitive and specific detection of the tagged protein using techniques like Western blotting or immunofluorescence. Furthermore, the FLAG tag is amenable to purification via affinity chromatography, where anti-FLAG antibodies are immobilized on a resin, enabling efficient and rapid purification of the tagged protein from complex biological samples. The versatility and efficiency of the DYDDDDK tag have revolutionized the field of molecular biology, aiding researchers in studying protein-protein interactions, subcellular localization, and protein dynamics, ultimately advancing our understanding of cellular processes and driving innovative discoveries in various scientific disciplines. |
Data
 |
| Immunofluorescence cell staining- rabbit anti-DYDDDDK tag polyclonal antibody 4849 Immunofluorescent analysis of paraformaldehyde (PFA) – fixed, permeabilized with 0.1% triton X-100 CHO cells transfected with GFP-3XDYKDDDK construct (CHO-GFP-2xFLAG) labeling DYKDDDDK tag with antibody #4849 at 5µg/mL, followed by Donkey Anti-rabbit IgG, Alexa 594 conjugated with 2.5µg/mL (red). Nucleus was counterstained with DAPI (blue). |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 2 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
| pmid | title | authors | citation |
|---|---|---|---|
| 35190665 | Intersectional, anterograde transsynaptic targeting of neurons receiving monosynaptic inputs from two upstream regions | Kitanishi T, Tashiro M, Kitanishi N, Mizuseki K. | Commun Biol. 2022 Feb 21;5(1):149. doi: 10.1038/s42003-022-03096-3. |
| 22234250 | The interaction of the von Hippel-Lindau tumor suppressor and heterochromatin protein 1 | Lai Y, Song M, Hakala K, Weintraub ST, Shiio Y. | Arch Biochem Biophys. 2012 Feb 15;518(2):103-10. doi: 10.1016/j.abb.2011.12.023. Epub 2012 Jan 3. |
Protocols
| relevant to this product |
|---|
| Western blot ICC |
Documents
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