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Entero RT-PCR test Mikrogen 830509

$487.00

Summary

  • Mikrogen diagnostik RT PCR kit for research use (RUO)
  • Direct Coxsackievirus, Echovirus, Poliovirus, Enterovirus detection
  • High sensitivity and specificity
  • Internal control for monitoring nucleic acid extraction
    (RNA/DNA) and real-time PCR inhibition in each reaction
  • Compatible with most common real-time PCR cyclers & RNA/DNA extraction methods
  • 96 tests
Weight 1 lbs
Dimensions 9 × 5 × 2 in
target

Entero

species reactivity

For the detection of enterovirus (Coxsackievirus A, Coxsackievirus B, Echovirus, Poliovirus).

applications

RT PCR

assay type

direct & qualitative

available sizes

96 tests

Entero RT-PCR test Mikrogen 830509

kit
Assay type
RT PCR
Research area
Infectious Disease
Sample type
whole blood, serum, plasma, urine, tissue, stool, etc., food and environmental samples or from the carrier material
Notes
Mic (Magnetic Induction Cycler) validated
Roche LightCycler(c) 480 Instrument II validated
Roche covas z 480 Analyzer validated
Qiagen Rotor-Gene(c) Q validated
Bio-Rad CFX 96 validated
Applied Biosystems (QuantStudio TM 5 Dx) validated
Stratagene Mx3000P compatible
Components
Reaction Mix Cap color - yellow 2 x 768 µl
Positive Control Cap color - red 1 x 100 µl
Negative Control Cap color - green 1 x 100 µl
Control DNA Cap color - colorless 2 x 240 µl
Instructions for Use 1 Each
Storage
Store at -20°C.
Additional information

Highly sensitive and specific direct detection of pathogens that can cause tick-borne infections Applicable to human starting material as well as RNA/DNA from the tick

Complete PCR kits with ready-to-use reagents

Compatible with common real-time PCR cyclers Compatible with various RNA/DNA extraction methods (e.g. Mikrogen alphaClean Mag RNA/DNA Kits)

target relevance
Organism
Coxsackievirus
Protein names
Coxsackievirus
Structure and strains
Coxsackieviruses are a few related enteroviruses that belong to the Picornaviridae family of nonenveloped, linear, positive-sense single-stranded RNA viruses, as well as its genus Enterovirus, which also includes poliovirus and echovirus. Enteroviruses are among the most common and important human pathogens, and ordinarily its members are transmitted by the fecal oral route. Coxsackieviruses share many characteristics with poliovirus. With control of poliovirus infections in much of the world, more attention has been focused on understanding the nonpolio enteroviruses such as coxsackievirus.

Coxsackieviruses are among the leading causes of aseptic meningitis (the other usual suspects being echovirus and mumps virus).

The entry of coxsackievirus into cells, especially endothelial cells, is mediated by coxsackievirus and adenovirus receptor.
Detection and diagnosis
The demonstration of infection by the detection of specific antibodies generally requires the analysis of serum pairs. A positive result for IgM or IgA combined with rising IgG activity serves as clear evidence of an acute or recent infection. Persisting IgM and IgA antibody levels are frequently observed in chronic infections.

Data

No results found

Publications

Publications

pmid title authors citation
We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
830509 protocol

Documents

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