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rabbit anti-VSV-G polyclonal antibody 1440

$426.00

Antibody summary

  • Rabbit polyclonal to VSV-G
  • Suitable for: ELISA,ICC,IHC,WB
  • Isotype: IgG
  • 100 µg
SKU: 1440parent Category: Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ELISA, ICC/IF, WB

reactivity

VSV-G

available sizes

100 µg

rabbit anti-VSV-G polyclonal antibody 1440

antibody
Tested applications
WB,ICC/IF,ELISA,IP
Recommended dilutions
Immunoblotting: use at a dilution of 1:1,000- 1:20,000.

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunoprecipitation: use 1-4ug/mg lysate.

ELISA: for detection use at a dilution of 1:1,000-1:30,000

These are recommended dilutions.

Endusers shoul
Immunogen
Synthetic peptide YTDIEMNRLGK conjugated to KLH.
Size and concentration
100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at 2-8°C.
Storage buffer
PBS, pH 7.2, 0.09% NaN3.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Biotechnology
The VSV-G tag has become a valuable and versatile tool in scientific research, particularly in molecular biology, cell biology, and virology studies. The VSV-G tag is derived from the glycoprotein of the Vesicular Stomatitis Virus (VSV), a virus that infects animals and does not replicate in mammalian cells. This feature makes the VSV-G tag highly useful for pseudotyping viral particles and creating viral vectors for gene delivery and expression studies. Researchers frequently use the VSV-G tag to replace the native envelope protein of viral vectors, enabling efficient and broad transduction of target cells, including difficult-to-transfect cell types. Furthermore, the VSV-G tag is often employed as a fusion tag for protein expression and purification, as it is well-tolerated and effectively trafficked to the plasma membrane. The VSV-G tag can be readily detected using specific antibodies in a variety of experimental assays, such as Western blotting and immunofluorescence, facilitating the visualization and quantification of tagged proteins. The widespread application of the VSV-G tag in research can be attributed to its utility in viral vector design, as well as its compatibility with multiple expression systems, making it a valuable tool for investigating cellular processes, gene function, and protein dynamics in diverse biological contexts.

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