rabbit anti-Vimentin monoclonal antibody (BLR100G) 9046

$409.00

Antibody summary

Rabbit monoclonal to Vimentin
Suitable for: WB, ICC/IF, IHC
Reacts with: human, mouse, rat
Isotype: IgG
100 µg

SKU: 9046 Categories: antibody, cell marker Tag: vimentin

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
available sizes	100 µg

rabbit anti-Vimentin monoclonal antibody (BLR100G) 9046

antibody
Database link: human P08670 mouse P20152 rat P31000
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions Western blot: 1:10000 IF/ICC and IHC: 1:5000
Immunogen Full length human TH expressed in and purified from E. coli.
Size and concentration 100µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit monoclonal - Isotype Control

target relevance
Homo sapiens VIM Vimentin
Protein names Vimentin
Gene names VIM
Protein family Belongs to the intermediate filament family
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity)
Subcellular location Cytoplasm, Cytoplasm, cytoskeleton, Nucleus matrix, Cell membrane, Cell projection, axon
Structure (Microbial infection) Interacts with Chandipura virus glycoprotein; this interaction might facilitate the binding of the virus to the cells
Post-translational modification Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm (PubMed:21465480). Phosphorylated by STK33 (PubMed:18811945). Phosphorylated on tyrosine residues by SRMS (PubMed:29496907) O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex
Involvement in disease Cataract 30, multiple types An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function.
Keywords 3D-structure, Acetylation, Cataract, Cell membrane, Cell projection, Coiled coil, Cytoplasm, Cytoskeleton, Direct protein sequencing, Disease variant, Glycoprotein, Host-virus interaction, Intermediate filament, Isopeptide bond, Membrane, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, S-nitrosylation, Ubl conjugation
Sequence MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGV YATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDK VRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAE DIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEE EIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSE AANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQD TIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSS LNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE
UniProt accession: P08670

Data

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Detection of human Vimentin in FFPE HeLa by ICC. Antibody: Rabbit anti-Vimentin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

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Detection of human Vimentin by western blot. Samples: Whole cell lysate (5 µg) from RKO, HeLa, MCF-7, U2OS, LNCaP, A-549, Hep-G2, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Vimentin recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

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Detection of human Vimentin in FFPE colon carcinoma by immunohistochemistry-IF. Antibody: Rabbit anti-Vimentin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.

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Detection of human Vimentin in FFPE tonsil by IHC.Antibody: Rabbit anti-Vimentin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB

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Detection of human Vimentin in FFPE colon by IHC.Antibody: Rabbit anti-Vimentin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB

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Detection of mouse Vimentin in FFPE renal cell carcinoma by IHC.Antibody: Rabbit anti-Vimentin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

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Detection of mouse Vimentin by western blot. Samples: Whole cell lysate (5 µg) from CH27, C2C12, 70Z/3, BW5147.3, CTLL-2, RenCa, J774A1, NIH 3T3, RAW 264.7, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Vimentin recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

FAQ & Publications

Frequently Asked Questions

What species does the rabbit anti-Vimentin monoclonal antibody (BLR100G) 9046 react with?

This antibody reacts with human, mouse, and rat Vimentin proteins.

Which applications is this anti-Vimentin monoclonal antibody validated for?

The antibody is suitable for Western blot (WB), immunocytochemistry/immunofluorescence (ICC/IF), and immunohistochemistry (IHC) applications.

How should the rabbit anti-Vimentin monoclonal antibody be stored to maintain its stability?

For short-term storage, keep the antibody at 2-8°C. For long-term storage, it should be stored at -20°C while avoiding freeze/thaw cycles.

What is the recommended dilution for using this antibody in Western blot and immunofluorescence assays?

For Western blot, a dilution of 1:10,000 is recommended. For immunofluorescence (IF), immunocytochemistry (ICC), and immunohistochemistry (IHC), the recommended dilution is 1:5,000.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.