rabbit anti-thioredoxin (TRX1) polyclonal antibody 1004

$100.00 – $2,600.00

Antibody summary

Rabbit polyclonal to Thioredoxin (Trx1)
Suitable for: IP,WB
Reacts with: Hu
Isotype: IgG
100 µg, 25 µg, 1 mg

SKU: 1004parent Categories: antibody, cell marker, oxidative stress Tags: polyclonal, rabbit, Thioredoxin (Trx)

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	IP, WB
reactivity	polyclonal, rabbit, TRX1
available sizes	1 mg, 100 µg, 25 µg

rabbit anti-TRX1 polyclonal antibody 1004

antibody
Database link: human P10599 mouse P10639 rat P11232
Tested applications WB,IP
Recommended dilutions WB: 1:2000-10000, IP 2 - 10 %micro;g/mg lysate
Size and concentration 100µL and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Thioredoxin (Trx) (ATL-derived factor) (ADF) (Surface-associated sulphydryl protein) (SASP) (allergen Hom s Trx)
Gene names TXN,TXN TRDX TRX TRX1
Protein family Thioredoxin family
Mass 11737Da
Function FUNCTION: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed:17182577, PubMed:19032234, PubMed:2176490). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed:16408020, PubMed:17606900). Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed:11118054, PubMed:9108029). {ECO:0000269\|PubMed:11118054, ECO:0000269\|PubMed:16408020, ECO:0000269\|PubMed:17182577, ECO:0000269\|PubMed:17606900, ECO:0000269\|PubMed:19032234, ECO:0000269\|PubMed:2176490, ECO:0000269\|PubMed:9108029}.; FUNCTION: ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000269\|PubMed:11118054, ECO:0000269\|PubMed:9108029}. Cytoplasm {ECO:0000269\|PubMed:11118054, ECO:0000269\|PubMed:9108029}. Secreted {ECO:0000269\|PubMed:1332947}. Note=Translocates from the cytoplasm into the nucleus after phorbol 12-myristate 13-acetate induction (PMA) (PubMed:9108029). Predominantly in the cytoplasm in non irradiated cells (PubMed:11118054). Radiation induces translocation of TRX from the cytoplasm to the nucleus (PubMed:11118054). Secreted by a leaderless secretory pathway (PubMed:1332947). {ECO:0000269\|PubMed:11118054, ECO:0000269\|PubMed:1332947, ECO:0000269\|PubMed:9108029}.
Structure SUBUNIT: Homodimer; disulfide-linked (PubMed:17260951, PubMed:9369469). Interacts with TXNIP through the redox-active site (PubMed:17260951). Interacts with MAP3K5 and CASP3 (PubMed:15246877). In case of infection, interacts with S.typhimurium protein slrP (PubMed:19690162). Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner (PubMed:9108029). {ECO:0000269\|PubMed:15246877, ECO:0000269\|PubMed:17260951, ECO:0000269\|PubMed:19690162, ECO:0000269\|PubMed:9108029, ECO:0000269\|PubMed:9369469}.
Post-translational modification PTM: In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins. {ECO:0000269\|PubMed:15246877, ECO:0000269\|PubMed:16408020, ECO:0000269\|PubMed:17260951, ECO:0000269\|PubMed:17606900}.; PTM: In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation. {ECO:0000269\|PubMed:19690162}.
Target Relevance information above includes information from UniProt accession : P10599
The UniProt Consortium

Data

WB-image-anti-Thioredoxin (Trx) antibody-1004_2

Detection of human Thioredoxin Reductase 1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Thioredoxin Reductase 1 antibody #1004 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

WB-image-anti-Thioredoxin (Trx) antibody-1004_4

Detection of human Thioredoxin Reductase 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Thioredoxin Reductase 1 antibody #1004 used for IP at 6 µg per reaction. Thioredoxin Reductase 1 was also immunoprecipitated by rabbit anti-Thioredoxin Reductase 1 antibody BL18351. For blotting immunoprecipitated Thioredoxin Reductase 1, #1802 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.