Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with anti-Topoisomerase IIa antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells

rabbit anti-Topoisomerase IIa monoclonal antibody (ZR94) 6388

$160.00 – $528.00

Antibody summary

Rabbit monoclonal to Topoisomerase IIa
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Tonsil
Visualization: Nucleus
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6388parent Category: antibody Tags: anatomy, General & Epithelial Markers, Topoisomerase IIa

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-Topoisomerase IIa monoclonal antibody ZR94 6388

antibody
Database link: human P11388
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Tonsil
Immunogen Synthetic peptide corresponding to Topo II ÃŽÂ± residues within aa1431-1531 of Topo II ÃŽÂ±
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names DNA topoisomerase 2-alpha (EC 5.6.2.2) (DNA topoisomerase II, alpha isozyme)
Gene names TOP2A,TOP2A TOP2
Protein family Type II topoisomerase family
Mass 174385Da
Function FUNCTION: Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity). {ECO:0000250\|UniProtKB:Q01320, ECO:0000269\|PubMed:17567603, ECO:0000269\|PubMed:18790802, ECO:0000269\|PubMed:22013166, ECO:0000269\|PubMed:22323612}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=ATP-dependent breakage, passage and rejoining of double-stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000255\|PROSITE-ProRule:PRU00995, ECO:0000269\|PubMed:19222228, ECO:0000269\|PubMed:19697956};
Subellular location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269\|PubMed:9155056}. Nucleus, nucleoplasm {ECO:0000269\|PubMed:8299728}. Nucleus {ECO:0000269\|PubMed:17567603, ECO:0000269\|PubMed:22013166, ECO:0000269\|PubMed:8299728, ECO:0000269\|PubMed:9155056}. Nucleus, nucleolus {ECO:0000269\|PubMed:9155056}.
Tissues TISSUE SPECIFICITY: Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung (PubMed:9155056). Also found in high-grade lymphomas, squamous cell lung tumors and seminomas (PubMed:9155056). {ECO:0000269\|PubMed:9155056}.
Structure SUBUNIT: Homodimer. Interacts with COPS5. Interacts with RECQL5; this stimulates DNA decatenation. Interacts with SETMAR; stimulates the topoisomerase activity (PubMed:18790802, PubMed:20457750). Interacts with DHX9; this interaction occurs in a E2 enzyme UBE2I- and RNA-dependent manner, negatively regulates DHX9-mediated double-stranded DNA and RNA duplex helicase activity and stimulates TOP2A-mediated supercoiled DNA relaxation activity (PubMed:12711669). Interacts with HNRNPU (via C-terminus); this interaction protects the topoisomerase TOP2A from degradation and positively regulates the relaxation of supercoiled DNA in a RNA-dependent manner (By similarity). Interacts with MCM3AP isoform GANP (PubMed:23652018). Interacts with ERCC6 (PubMed:26030138). Interacts with PLSCR1 (PubMed:17567603). Interacts with GCNA; this interaction allows the resolution of topoisomerase II (TOP2A) DNA-protein cross-links (By similarity). Interacts with POL1RA/RPA1 (via dock II) and UBTF in the context of Pol I complex; may assist Pol I transcription initiation by releasing supercoils occurring during DNA unwinding. Interacts with TPRN; TPRN interacts with a number of DNA damage response proteins, is recruited to sites of DNA damage and may play a role in DNA damage repair (PubMed:23213405). {ECO:0000250\|UniProtKB:P41516, ECO:0000250\|UniProtKB:Q01320, ECO:0000269\|PubMed:12711669, ECO:0000269\|PubMed:15126503, ECO:0000269\|PubMed:16100112, ECO:0000269\|PubMed:17567603, ECO:0000269\|PubMed:20457750, ECO:0000269\|PubMed:22013166, ECO:0000269\|PubMed:22841979, ECO:0000269\|PubMed:23213405, ECO:0000269\|PubMed:23652018, ECO:0000269\|PubMed:26030138, ECO:0000269\|PubMed:36271492}.
Post-translational modification PTM: Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation. {ECO:0000269\|PubMed:10942766, ECO:0000269\|PubMed:18062778, ECO:0000269\|PubMed:19043076, ECO:0000269\|Ref.11}.; PTM: (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1; leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks. {ECO:0000269\|PubMed:34543352}.; PTM: SUMOylated. {ECO:0000269\|PubMed:34543352}.
Domain DOMAIN: The N-terminus has several structural domains; the ATPase domain (about residues 1-265), the transducer domain (about 266-428) and the toprim domain (455-572) (PubMed:25202966). Comparing different structures shows ATP hydrolysis induces domain shifts in the N-terminus that are probably part of the mechanism of DNA cleavage and rejoining (PubMed:25202966). {ECO:0000250\|UniProtKB:P0AES6, ECO:0000269\|PubMed:25202966}.
Target Relevance information above includes information from UniProt accession: P11388
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with anti-Topoisomerase IIa antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells

Publications

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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.