Immunohistochemistry (IHC) : Human urothelial carcinoma stained with anti-thymidylate synthase (TS) antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells.

rabbit anti-Thymidylate Synthase (TS) monoclonal antibody (ZR245) 6381

Price range: $160.00 through $528.00

Antibody summary

Rabbit monoclonal to Thymidylate Synthase (TS)
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: 5-FU resistant cancer
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6381parent Categories: antibody, Zeta IHC antibodies Tags: anatomy, General & Epithelial Markers, Thymidylate Synthase (TS)

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-Thymidylate Synthase (TS) monoclonal antibody ZR245 6381

antibody
Database link: human P04818
Tested applications IHC
Recommended dilutions Concentrated 1:50
Application Notes Positive control: 5-FU resistant cancer
Immunogen Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Homo sapiens TYMS Thymidylate synthase
Protein names Thymidylate synthase
Gene names TYMS
Protein family Belongs to the thymidylate synthase family
Function Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the mitochondrial and nuclear de novo thymidylate biosynthesis pathway
Catalytic activity dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP
Subcellular location Nucleus, Cytoplasm, Mitochondrion, Mitochondrion matrix, Mitochondrion inner membrane
Structure Homodimer. Part of the de novo thymidylate synthesis complex composed at least by SHMT1, DHFR and TYMS (PubMed:22235121). Interacts with SHMT1; this interaction is DNA-dependent and mediates TYMS co-localization with LMNB1 (PubMed:22235121)
Post-translational modification Sumoylated by UBE2I/UBC9
Involvement in disease Dyskeratosis congenita, digenic A form of dyskeratosis congenita, a rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy. DKCD transmission pattern is consistent with digenic inheritance.
Keywords 3D-structure, Alternative splicing, Cytoplasm, Direct protein sequencing, Disease variant, Dyskeratosis congenita, Isopeptide bond, Membrane, Methyltransferase, Mitochondrion, Mitochondrion inner membrane, Nucleotide biosynthesis, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Transferase, Ubl conjugation
Sequence MPVAGSELPRRPLPPAAQERDAEPRPPHGELQYLGQIQHILRCGVRKDDRTGTGTLSVFG MQARYSLRDEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVKIWDANGSRDFLDS LGFSTREEGDLGPVYGFQWRHFGAEYRDMESDYSGQGVDQLQRVIDTIKTNPDDRRIIMC AWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHI TGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIEG YNPHPTIKMEMAV
UniProt accession: P04818

Data

Human urothelial carcinoma stained with anti-thymidylate synthase (TS) antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells.

FAQ & Publications

Frequently Asked Questions

What species does the rabbit anti-Thymidylate Synthase (TS) monoclonal antibody (ZR245) specifically react with?

This antibody is reactive with human species only.

Which applications is the rabbit anti-Thymidylate Synthase (TS) antibody validated for?

It is suitable for Immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded tissues.

How should the rabbit anti-Thymidylate Synthase (TS) monoclonal antibody be stored to maintain stability?

For short-term storage, keep the antibody at 2-8°C. For long-term storage, freeze at -20°C and avoid repeated freeze/thaw cycles.

What is the recommended dilution for using the concentrated form of this antibody in immunohistochemistry?

The recommended dilution for the concentrated antibody is 1:50 for IHC applications.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.