rabbit anti-SOD1 polyclonal antibody 1001

$100.00 – $2,600.00

Antibody summary

Rabbit polyclonal to Superoxide Dismutase 1 (SOD)
Suitable for: WB,IHC
Reacts with: Hu
Isotype: IgG
100 µg, 25 µg, 1 mg

SKU: 1001parent Categories: antibody, cell marker, oxidative stress Tags: polyclonal, rabbit, Superoxide Dismutase 1 (SOD)

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	IHC, WB
reactivity	polyclonal, rabbit, Superoxide Dismutase 1 (SOD)
available sizes	1 mg, 100 µg, 25 µg

rabbit anti- sod1 polyclonal antibody 1001

antibody
Database link: human P00441 mouse P08228 rat P07632
Tested applications WB,IHC
Recommended dilutions WB: 1:2000-10000, IHC: 1:2000-10000, Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunogen The epitope recognized by A303-811A maps to a region between residue 54 and 104 of human Superoxide Dismutase 1 using the numbering given in entry NP_000445.1 (GeneID 6647).
Size and concentration 100µL and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Superoxide dismutase [Cu-Zn] (EC 1.15.1.1) (Superoxide dismutase 1) (hSod1)
Gene names SOD1,SOD1
Protein family Cu-Zn superoxide dismutase family
Mass 15936Da
Function FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. {ECO:0000269\|PubMed:24140062}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=2 H(+) + 2 superoxide = H2O2 + O2; Xref=Rhea:RHEA:20696, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:18421; EC=1.15.1.1; Evidence={ECO:0000269\|PubMed:24140062};
Subellular location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269\|PubMed:19741096}. Nucleus {ECO:0000269\|PubMed:22496122}. Note=Predominantly cytoplasmic; the pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria. {ECO:0000269\|PubMed:19741096}.
Structure SUBUNIT: Homodimer; non-disulfide-linked (By similarity). Homodimerization may take place via the ditryptophan cross-link at Trp-33. Heterodimer with SOD1 (PubMed:31292775). The heterodimer CCS:SOD1 interacts with SLC31A1; this heterotrimer is Cu(1+)-mediated and its maintenance is regulated through SOD1 activation (PubMed:31292775). Interacts with DAOA; the interaction is direct (PubMed:30037290). {ECO:0000250\|UniProtKB:P08228, ECO:0000269\|PubMed:10329151, ECO:0000269\|PubMed:12911296, ECO:0000269\|PubMed:12963370, ECO:0000269\|PubMed:15326189, ECO:0000269\|PubMed:16291742, ECO:0000269\|PubMed:16406071, ECO:0000269\|PubMed:17548825, ECO:0000269\|PubMed:17888947, ECO:0000269\|PubMed:20600836, ECO:0000269\|PubMed:20727846, ECO:0000269\|PubMed:30037290, ECO:0000269\|PubMed:31292775}.
Post-translational modification PTM: Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation. {ECO:0000269\|PubMed:12145308, ECO:0000269\|PubMed:19741096}.; PTM: The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. {ECO:0000269\|PubMed:20600836}.; PTM: Palmitoylation helps nuclear targeting and decreases catalytic activity. {ECO:0000269\|PubMed:22496122}.; PTM: Succinylation, adjacent to copper catalytic site, probably inhibits activity. Desuccinylation by SIRT5 enhances activity. {ECO:0000269\|PubMed:24140062}.
Target Relevance information above includes information from UniProt accession : P00441
The UniProt Consortium

Data

WB-image-anti-Superoxide Dismutase 1 (SOD) antibody-1001_1

Detection of human SOD1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SOD1 antibody #1001 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

IHC-image-anti-Superoxide Dismutase 1 (SOD) antibody-1001_2

Detection of human SOD1 by immunohistochemistry. Sample: FFPE section of human osteosarcoma. Antibody: Affinity purified rabbit anti-SOD1 (#1001) used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.