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rabbit anti-SIRP α (CT) polyclonal antibody 9535

$478.00

Antibody summary

  • Rabbit polyclonal to SIRP α (CT)
  • Suitable for: ELISA,WB,ICC
  • Isotype: IgG
  • 100 µg
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SKU: 9535parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

SIRPα

available sizes

100 µg

antibody
Tested applications
WB,ICC/IF,ELISA
Recommended dilutions
Immunoblotting: use at 0.5-2ug/mL. A band of 75-110kDa is detected.

Immunocytochemistry: use at 1-5ug/mL.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
Immunogen
Synthetic peptide corresponding to 17 amino acids within the last 50 amino acids at the C-terminus of human SIRPa.
Size and concentration
100µg and 1 mg/mL
Form
liquid
Storage Instructions
This antibody is stable at 4°C for three months and for at least one year at -20ËšC. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, 0.02% NaN3.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Tyrosine-protein phosphatase non-receptor type substrate 1 (SHP substrate 1) (SHPS-1) (Brain Ig-like molecule with tyrosine-based activation motifs) (Bit) (CD172 antigen-like family member A) (Inhibitory receptor SHPS-1) (Macrophage fusion receptor) (MyD-1 antigen) (Signal-regulatory protein alpha-1) (Sirp-alpha-1) (Signal-regulatory prot
Gene names
SIRPA,SIRPA BIT MFR MYD1 PTPNS1 SHPS1 SIRP
Mass
54967Da
Function
FUNCTION: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Plays a role in antiviral immunity and limits new world arenavirus infection by decreasing virus internalization (By similarity). Receptor for THBS1 (PubMed:24511121). Interaction with THBS1 stimulates phosphorylation of SIRPA (By similarity). In response to THBS1, involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production (PubMed:24511121). {ECO:0000250, ECO:0000250|UniProtKB:P97710, ECO:0000269|PubMed:10469599, ECO:0000269|PubMed:11509594, ECO:0000269|PubMed:24511121}.
Subellular location
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
Tissues
TISSUE SPECIFICITY: Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
Structure
SUBUNIT: Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR (By similarity). Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB1. Binds PTK2B. Interacts with TRIM2 (By similarity). {ECO:0000250, ECO:0000269|PubMed:18657508, ECO:0000269|PubMed:24511121}.
Post-translational modification
PTM: N-glycosylated. {ECO:0000269|PubMed:16335952, ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:9062191}.; PTM: Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. {ECO:0000269|PubMed:10842184, ECO:0000269|PubMed:9062191}.
Domain
TOPO_DOM 3
Target Relevance information above includes information from UniProt accession : P78324
The UniProt Consortium

Data

benchmark-antibodies_anti-sirpalpha_antibody_9535_1.jpg
Western blot analysis of SIRP alpha in THP-1 whole cell lysate with SIRP alpha antibody at 0.5 µg/mL.
benchmark-antibodies_anti-sirpalpha_antibody_9535_2.jpg
Immunocytochemistry of SIRP alpha in THP-1 cells with SIRP alpha antibody at 1 µg/mL.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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