| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | SIRPα |
| available sizes | 100 µg |
rabbit anti-SIRP α (CT) polyclonal antibody 9535
$478.00
Antibody summary
- Rabbit polyclonal to SIRP α (CT)
- Suitable for: ELISA,WB,ICC
- Isotype: IgG
- 100 µg
rabbit anti-SIRP α (CT) polyclonal antibody 9535
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at 0.5-2ug/mL. A band of 75-110kDa is detected. Immunocytochemistry: use at 1-5ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications. |
| Immunogen Synthetic peptide corresponding to 17 amino acids within the last 50 amino acids at the C-terminus of human SIRPa. |
| Size and concentration 100µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable at 4°C for three months and for at least one year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, 0.02% NaN3. |
| Purity peptide affinty purifcation |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Tyrosine-protein phosphatase non-receptor type substrate 1 (SHP substrate 1) (SHPS-1) (Brain Ig-like molecule with tyrosine-based activation motifs) (Bit) (CD172 antigen-like family member A) (Inhibitory receptor SHPS-1) (Macrophage fusion receptor) (MyD-1 antigen) (Signal-regulatory protein alpha-1) (Sirp-alpha-1) (Signal-regulatory prot |
| Gene names SIRPA,SIRPA BIT MFR MYD1 PTPNS1 SHPS1 SIRP |
| Mass 54967Da |
| Function FUNCTION: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Plays a role in antiviral immunity and limits new world arenavirus infection by decreasing virus internalization (By similarity). Receptor for THBS1 (PubMed:24511121). Interaction with THBS1 stimulates phosphorylation of SIRPA (By similarity). In response to THBS1, involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production (PubMed:24511121). {ECO:0000250, ECO:0000250|UniProtKB:P97710, ECO:0000269|PubMed:10469599, ECO:0000269|PubMed:11509594, ECO:0000269|PubMed:24511121}. |
| Subellular location SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. |
| Tissues TISSUE SPECIFICITY: Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. |
| Structure SUBUNIT: Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR (By similarity). Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB1. Binds PTK2B. Interacts with TRIM2 (By similarity). {ECO:0000250, ECO:0000269|PubMed:18657508, ECO:0000269|PubMed:24511121}. |
| Post-translational modification PTM: N-glycosylated. {ECO:0000269|PubMed:16335952, ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:9062191}.; PTM: Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. {ECO:0000269|PubMed:10842184, ECO:0000269|PubMed:9062191}. |
| Domain TOPO_DOM 3 |
| Target Relevance information above includes information from UniProt accession : P78324 |
| The UniProt Consortium |
Data
 |
| Western blot analysis of SIRP alpha in THP-1 whole cell lysate with SIRP alpha antibody at 0.5 µg/mL. |
 |
| Immunocytochemistry of SIRP alpha in THP-1 cells with SIRP alpha antibody at 1 µg/mL. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| Western blot IHC ICC |
Documents
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