Immunohistochemistry (IHC) : Formalin-fixed and paraffin-embedded human head and neck small cell carcinoma stained with anti-RRM1 antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells

rabbit anti-RRM1 monoclonal antibody (ZR114) 6356

Price range: $160.00 through $528.00

Antibody summary

Rabbit monoclonal to RRM1
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Breast or colon carcinoma
Visualization: cytoplasm
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

available sizes

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SKU: 6356parent Category: antibody Tags: anatomy, General & Epithelial Markers, RRM1

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-RRM1 monoclonal antibody ZR114 6356

antibody
Database link: human P23921
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Breast or colon carcinoma
Immunogen Synthetic peptide corresponding to residues within aa700-800 of human RRM1
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Ribonucleoside-diphosphate reductase large subunit (EC 1.17.4.1) (Ribonucleoside-diphosphate reductase subunit M1) (Ribonucleotide reductase large subunit)
Gene names RRM1,RRM1 RR1
Protein family Ribonucleoside diphosphate reductase large chain family
Mass 90070Da
Function FUNCTION: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Catalytic activity CATALYTIC ACTIVITY: Reaction=a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol; Xref=Rhea:RHEA:23252, Rhea:RHEA-COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:29950, ChEBI:CHEBI:50058, ChEBI:CHEBI:57930, ChEBI:CHEBI:73316; EC=1.17.4.1; Evidence={ECO:0000269\|PubMed:16376858};
Subellular location SUBCELLULAR LOCATION: Cytoplasm.
Structure SUBUNIT: Heterodimer of a large and a small subunit. Heterodimer with small subunit RRM2 or RRM2B. The heterodimer with RRM2 has higher catalytic activity than the heterodimer with RRM2B. Interacts with AHCYL1 which inhibits its activity. {ECO:0000269\|PubMed:16376858, ECO:0000269\|PubMed:21336276, ECO:0000269\|PubMed:25237103}.
Involvement in disease DISEASE: Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 6 (PEOB6) [MIM:620647]: A form of progressive external ophthalmoplegia, a mitochondrial myopathy characterized by progressive paralysis of the levator palpebrae, orbicularis oculi, and extraocular muscles. Ragged red fibers are seen on muscle biopsy. {ECO:0000269\|PubMed:35617047}. Note=The disease may be caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P23921
The UniProt Consortium

Data

Formalin-fixed and paraffin-embedded human head and neck small cell carcinoma stained with anti-RRM1 antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.