rabbit anti-PTEN (Phospho-Ser380) polyclonal antibody 7843

$366.00

Antibody summary

Rabbit polyclonal to PTEN (Phospho-Ser380)
Suitable for: IF,IHC
Isotype: Whole IgG
100 µl

SKU: 7843parent Category: antibody Tags: polyclonal, PTEN (Phospho-Ser380), rabbit

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	PTEN (Phospho-Ser380)
available sizes	100 µL

rabbit anti-PTEN (Phospho-Ser380) polyclonal antibody 7843

antibody

Tested applications IHC,IHC
Recommended dilutions Immunofluorescence: use at dilution of 1:100- 1:200. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications.
Immunogen Peptide sequence that includes phosphorylation site of Serine 380 derived from human PTEN and conjugated to KLH.
Size and concentration 100µL and 1 mg/mL
Form liquid
Storage Instructions This antibody is stable for at least one (1) year at -20°C.
Storage buffer PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl,
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (EC 3.1.3.16) (EC 3.1.3.48) (EC 3.1.3.67) (Inositol polyphosphate 3-phosphatase) (EC 3.1.3.-) (Mutated in multiple advanced cancers 1) (Phosphatase and tensin homolog)
Gene names PTEN,PTEN MMAC1 TEP1
Protein family PTEN phosphatase protein family
Mass 47166Da
Function FUNCTION: Dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins (PubMed:9187108, PubMed:9256433, PubMed:9616126). Also functions as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring of PtdIns(3,4,5)P3/phosphatidylinositol 3,4,5-trisphosphate, PtdIns(3,4)P2/phosphatidylinositol 3,4-diphosphate and PtdIns3P/phosphatidylinositol 3-phosphate with a preference for PtdIns(3,4,5)P3 (PubMed:9811831, PubMed:16824732, PubMed:26504226, PubMed:9593664). Furthermore, this enzyme can also act as a cytosolic inositol 3-phosphatase acting on Ins(1,3,4,5,6)P5/inositol 1,3,4,5,6 pentakisphosphate and possibly Ins(1,3,4,5)P4/1D-myo-inositol 1,3,4,5-tetrakisphosphate (PubMed:11418101, PubMed:15979280). Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival (PubMed:31492966, PubMed:37279284). The unphosphorylated form cooperates with MAGI2 to suppress AKT1 activation (PubMed:11707428). In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement (PubMed:22279049). Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation (PubMed:22279049). Required for growth factor-induced epithelial cell migration; growth factor stimulation induces PTEN phosphorylation which changes its binding preference from the p85 regulatory subunit of the PI3K kinase complex to DLC1 and results in translocation of the PTEN-DLC1 complex to the posterior of migrating cells to promote RHOA activation (PubMed:26166433). Meanwhile, TNS3 switches binding preference from DLC1 to p85 and the TNS3-p85 complex translocates to the leading edge of migrating cells to activate RAC1 activation (PubMed:26166433). Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). Involved in the regulation of synaptic function in excitatory hippocampal synapses. Recruited to the postsynaptic membrane upon NMDA receptor activation, is required for the modulation of synaptic activity during plasticity. Enhancement of lipid phosphatase activity is able to drive depression of AMPA receptor-mediated synaptic responses, activity required for NMDA receptor-dependent long-term depression (LTD) (By similarity). May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability (PubMed:10468583, PubMed:18716620). {ECO:0000250\|UniProtKB:O08586, ECO:0000250\|UniProtKB:O54857, ECO:0000269\|PubMed:10468583, ECO:0000269\|PubMed:11418101, ECO:0000269\|PubMed:11707428, ECO:0000269\|PubMed:15979280, ECO:0000269\|PubMed:16824732, ECO:0000269\|PubMed:18716620, ECO:0000269\|PubMed:22279049, ECO:0000269\|PubMed:26166433, ECO:0000269\|PubMed:26504226, ECO:0000269\|PubMed:31492966, ECO:0000269\|PubMed:37279284, ECO:0000269\|PubMed:9187108, ECO:0000269\|PubMed:9256433, ECO:0000269\|PubMed:9593664, ECO:0000269\|PubMed:9616126, ECO:0000269\|PubMed:9811831}.; FUNCTION: [Isoform alpha]: Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1. {ECO:0000269\|PubMed:23744781}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphate; Xref=Rhea:RHEA:25017, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:57836, ChEBI:CHEBI:58456; EC=3.1.3.67; Evidence={ECO:0000269\|PubMed:16824732, ECO:0000269\|PubMed:9811831}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:25018; Evidence={ECO:0000305\|PubMed:16824732}; CATALYTIC ACTIVITY: Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] + phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474, ChEBI:CHEBI:83421; EC=3.1.3.16; Evidence={ECO:0000269\|PubMed:9256433}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20630; Evidence={ECO:0000305\|PubMed:9256433}; CATALYTIC ACTIVITY: Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] + phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474, ChEBI:CHEBI:61977; EC=3.1.3.16; Evidence={ECO:0000269\|PubMed:9256433}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47005; Evidence={ECO:0000305\|PubMed:9256433}; CATALYTIC ACTIVITY: Reaction=H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate; Xref=Rhea:RHEA:10684, Rhea:RHEA-COMP:10136, Rhea:RHEA-COMP:10137, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:46858, ChEBI:CHEBI:82620; EC=3.1.3.48; Evidence={ECO:0000269\|PubMed:9187108, ECO:0000269\|PubMed:9256433}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:10685; Evidence={ECO:0000305\|PubMed:9256433}; CATALYTIC ACTIVITY: Reaction=1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphate; Xref=Rhea:RHEA:43552, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:83416, ChEBI:CHEBI:83419; Evidence={ECO:0000269\|PubMed:16824732, ECO:0000269\|PubMed:9593664}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43553; Evidence={ECO:0000305\|PubMed:16824732}; CATALYTIC ACTIVITY: Reaction=1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphate; Xref=Rhea:RHEA:43560, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:83420, ChEBI:CHEBI:83423; Evidence={ECO:0000269\|PubMed:16824732, ECO:0000269\|PubMed:9593664}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43561; Evidence={ECO:0000305\|PubMed:16824732}; CATALYTIC ACTIVITY: Reaction=1D-myo-inositol 1,3,4,5,6-pentakisphosphate + H2O = 1D-myo-inositol 1,4,5,6-tetrakisphosphate + phosphate; Xref=Rhea:RHEA:77143, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:57627, ChEBI:CHEBI:57733; Evidence={ECO:0000269\|PubMed:11418101}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:77144; Evidence={ECO:0000269\|PubMed:11418101}; CATALYTIC ACTIVITY: Reaction=1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + phosphate; Xref=Rhea:RHEA:77155, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:57895, ChEBI:CHEBI:203600; Evidence={ECO:0000269\|PubMed:9593664}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:77156; Evidence={ECO:0000305\|PubMed:9593664};
Subellular location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269\|PubMed:15987703, ECO:0000269\|PubMed:18716620, ECO:0000269\|PubMed:19473982, ECO:0000269\|PubMed:25801959, ECO:0000269\|PubMed:9187108}. Nucleus {ECO:0000269\|PubMed:15987703, ECO:0000269\|PubMed:18716620, ECO:0000269\|PubMed:19473982, ECO:0000269\|PubMed:25801959}. Nucleus, PML body {ECO:0000269\|PubMed:18716620}. Cell projection, dendritic spine {ECO:0000250\|UniProtKB:O54857}. Postsynaptic density {ECO:0000250\|UniProtKB:O54857}. Note=Monoubiquitinated form is nuclear. Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies (PubMed:18716620). XIAP/BIRC4 promotes its nuclear localization (PubMed:19473982). Associares with the postsynaptic density in response to NMDAR activation (By similarity). {ECO:0000250\|UniProtKB:O54857, ECO:0000269\|PubMed:18716620, ECO:0000269\|PubMed:19473982}.; SUBCELLULAR LOCATION: [Isoform alpha]: Secreted {ECO:0000269\|PubMed:23744781, ECO:0000269\|PubMed:24768297}. Note=May be secreted via a classical signal peptide and reenter into cells with the help of a poly-Arg motif. {ECO:0000269\|PubMed:23744781, ECO:0000269\|PubMed:24768297}.
Tissues TISSUE SPECIFICITY: Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas. {ECO:0000269\|PubMed:9090379}.
Structure SUBUNIT: Monomer. The unphosphorylated form interacts with the second PDZ domain of MAGI2 and with DLG1 and MAST2 in vitro (PubMed:10646847, PubMed:10760291, PubMed:11707428). Interacts with MAGI2, MAGI3, MAST1 and MAST3, but neither with MAST4 nor with DLG5; interaction with MAGI2 increases protein stability (PubMed:10748157, PubMed:15951562). Interacts with NEDD4 (PubMed:17218260). Interacts with NDFIP1 and NDFIP2; in the presence of NEDD4 or ITCH, this interaction promotes PTEN ubiquitination (PubMed:25801959, PubMed:20534535). Interacts (via C2 domain) with FRK (PubMed:19345329). Interacts with USP7; the interaction is direct (PubMed:18716620). Interacts with ROCK1 (By similarity). Interacts with XIAP/BIRC4 (PubMed:19473982). Interacts with STK11; the interaction phosphorylates PTEN (PubMed:15987703). Interacts with PPP1R16B (PubMed:25007873). Interacts with NOP53; regulates PTEN phosphorylation and increases its stability (PubMed:15355975). Interacts (via PDZ domain-binding motif) with DLG4; the interaction is induced by NMDA and is required for PTEN location at postsynaptic density (By similarity). Interacts with the regulatory p85 subunit of the PI3K kinase complex and with Rho GTPase-activating protein DLC1; in resting cells, interacts (via C2 tensin-type domain) with p85 but, following growth factor stimulation, PTEN is phosphorylated which leads to weakened interaction with p85 and enhanced interaction (via C2 tensin-type domain) with DLC1 while p85 interaction with TNS3 increases (PubMed:26166433). {ECO:0000250\|UniProtKB:O08586, ECO:0000250\|UniProtKB:O54857, ECO:0000269\|PubMed:10555148, ECO:0000269\|PubMed:10646847, ECO:0000269\|PubMed:10748157, ECO:0000269\|PubMed:10760291, ECO:0000269\|PubMed:11707428, ECO:0000269\|PubMed:15355975, ECO:0000269\|PubMed:15951562, ECO:0000269\|PubMed:15987703, ECO:0000269\|PubMed:17218260, ECO:0000269\|PubMed:18716620, ECO:0000269\|PubMed:19345329, ECO:0000269\|PubMed:19473982, ECO:0000269\|PubMed:20534535, ECO:0000269\|PubMed:25007873, ECO:0000269\|PubMed:25801959, ECO:0000269\|PubMed:26166433}.
Post-translational modification PTM: Constitutively phosphorylated by CK2 under normal conditions. Phosphorylated in vitro by MAST1, MAST2, MAST3 and STK11. Phosphorylation results in an inhibited activity towards PIP3. Phosphorylation can both inhibit or promote PDZ-binding. Phosphorylation at Tyr-336 by FRK/PTK5 protects this protein from ubiquitin-mediated degradation probably by inhibiting its binding to NEDD4. Phosphorylation by ROCK1 is essential for its stability and activity. Phosphorylation by PLK3 promotes its stability and prevents its degradation by the proteasome. Phosphorylated on Thr-319 and Thr-321 in the C2-type tensin domain following EGF stimulation which changes its binding preference from the p85 regulatory subunit of the PI3K kinase complex to DLC1 (PubMed:26166433). {ECO:0000269\|PubMed:10646847, ECO:0000269\|PubMed:11035045, ECO:0000269\|PubMed:11707428, ECO:0000269\|PubMed:12297295, ECO:0000269\|PubMed:15951562, ECO:0000269\|PubMed:15987703, ECO:0000269\|PubMed:18716620, ECO:0000269\|PubMed:19345329, ECO:0000269\|PubMed:20940307, ECO:0000269\|PubMed:26166433}.; PTM: Monoubiquitinated; monoubiquitination is increased in presence of retinoic acid. Deubiquitinated by USP7; leading to its nuclear exclusion. Monoubiquitination of one of either Lys-13 and Lys-289 amino acid is sufficient to modulate PTEN compartmentalization. Ubiquitinated by XIAP/BIRC4. {ECO:0000269\|PubMed:18716620, ECO:0000269\|PubMed:19473982}.; PTM: Ubiquitinated by the DCX(DCAF13) E3 ubiquitin ligase complex, leading to its degradation. {ECO:0000269\|PubMed:31492966}.; PTM: ISGylated. ISGylation promotes PTEN degradation. {ECO:0000269\|PubMed:37279284}.
Target Relevance information above includes information from UniProt accession : P60484
The UniProt Consortium

Data

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Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.