Immunohistochemistry (IHC) : Human rhabdomyosarcoma stained with anti-MyoD1 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of sarcoma cells.

rabbit anti-MyoD1 monoclonal antibody (ZR262) 6277

$160.00 – $528.00

Antibody summary

Rabbit monoclonal to MyoD1
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Rhabdomyosarcoma
Visualization: Nuclear and cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6277parent Category: antibody Tags: anatomy, monoclonal, MyoD1, Soft Tissue Pathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-MyoD1 monoclonal antibody ZR262 6277

antibody
Database link: human P15172
Tested applications IHC
Recommended dilutions As directed
Immunogen Synthetic peptide corresponding to residues within aa 1-100 of human MyoD1
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Myoblast determination protein 1 (Class C basic helix-loop-helix protein 1) (bHLHc1) (Myogenic factor 3) (Myf-3)
Mass 34501Da
Function Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity). {ECO:0000250}.
Subellular location Nucleus.
Structure Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1. Interacts with DDX5. Interacts with CHD2. Interacts with TSC22D3 (By similarity). Interacts with SETD3 (By similarity). Interacts with P-TEFB complex; promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation (By similarity) (PubMed:12037670). Interacts with CSRP3. Interacts with NUPR1 (By similarity). {ECO:0000250\|UniProtKB:P10085, ECO:0000269\|PubMed:12037670, ECO:0000269\|PubMed:16858404, ECO:0000269\|PubMed:24860983}.
Post-translational modification Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation. {ECO:0000269\|PubMed:12037670}.; Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity). {ECO:0000250}.; Ubiquitinated on the N-terminus; which is required for proteasomal degradation. {ECO:0000269\|PubMed:9774340}.; Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity. {ECO:0000269\|PubMed:22215600}.
Involvement in disease DISEASE: Congenital myopathy 17 (CMYP17) [MIM:618975]: An autosomal recessive muscular disorder characterized by hypotonia and respiratory insufficiency apparent soon after birth, high diaphragmatic dome on imaging, poor overall growth, pectus excavatum, dysmorphic facies, and renal anomalies in some affected individuals. Additional variable features include delayed motor development, mildly decreased endurance, distal arthrogryposis, and lung hypoplasia resulting in early death. {ECO:0000269\|PubMed:26733463, ECO:0000269\|PubMed:30403323, ECO:0000269\|PubMed:31260566}. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P15172
The UniProt Consortium

Data

Human rhabdomyosarcoma stained with anti-MyoD1 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of sarcoma cells.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.