Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human normal colon stained with anti-MUC-2 antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells

rabbit anti-MUC-2 monoclonal antibody (ZR175) 6267

Price range: $160.00 through $528.00

Antibody summary

Rabbit monoclonal to MUC-2
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Colon carcinoma
Visualization: Cell membrane
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6267parent Categories: antibody, Zeta IHC antibodies Tags: anatomy, GI Pathology, monoclonal, MUC-2

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-MUC-2 monoclonal antibody ZR175 6267

antibody
Database link: human Q02817
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Colon carcinoma
Immunogen Synthetic peptide representing a region on the Muc-2 glycoprotein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Homo sapiens MUC2 Mucin-2
Protein names Mucin-2
Alternative names Intestinal mucin-2
Gene names MUC2
Function Coats the epithelia of the intestines and other mucus membrane-containing organs to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces (PubMed:17058067, PubMed:19432394, PubMed:33031746). Major constituent of the colon mucus, which is mainly formed by large polymeric networks of MUC2 secreted by goblet cells that cover the exposed surfaces of intestine (PubMed:19432394, PubMed:33031746). MUC2 networks form hydrogels that guard the underlying epithelium from pathogens and other hazardous matter entering from the outside world, while permitting nutrient absorption and gas exchange (PubMed:33031746, PubMed:36206754). Acts as a divalent copper chaperone that protects intestinal cells from copper toxicity and facilitates nutritional copper unptake into cells (PubMed:36206754). Binds both Cu(2+) and its reduced form, Cu(1+), at two juxtaposed binding sites: Cu(2+), once reduced to Cu(1+) by vitamin C (ascorbate) or other dietary antioxidants, transits to the other binding site (PubMed:36206754). MUC2-bound Cu(1+) is protected from oxidation in aerobic environments, and can be released for nutritional delivery to cells (PubMed:36206754). Mucin gels store antimicrobial molecules that participate in innate immunity (PubMed:33031746). Mucin glycoproteins also house and feed the microbiome, lubricate tissue surfaces, and may facilitate the removal of contaminants and waste products from the body (PubMed:33031746). Goblet cells synthesize two forms of MUC2 mucin that differ in branched chain O-glycosylation and the site of production in the colon: a (1) 'thick' mucus that wraps the microbiota to form fecal pellets is produced in the proximal, ascending colon (By similarity). 'Thick' mucus transits along the descending colon and is lubricated by a (2) 'thin' MUC2 mucus produced in the distal colon which adheres to the 'thick' mucus (By similarity)
Subcellular location Secreted
Structure (Microbial infection) Interacts in vitro with L.monocytogenes internalin proteins InlB, InlC and InlJ; for InlC binding is slightly better at pH 5.5, (the pH of the intestine) than at pH 7.4
Post-translational modification O-glycosylated (PubMed:11445551, PubMed:33031746). O-glycosylation is required for mucin assembly (PubMed:33031746). Goblet cells synthesize two forms of mucin that differ in branched chain O-glycosylation and the site of production in the colon (By similarity) May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway
Keywords 3D-structure, Autocatalytic cleavage, Calcium, Copper, Disulfide bond, Glycoprotein, Phosphoprotein, Proteomics identification, Reference proteome, Repeat, Secreted, Signal
Sequence MGLPLARLAAVCLALSLAGGSELQTEGRTRNHGHNVCSTWGNFHYKTFDGDVFRFPGLCD YNFASDCRGSYKEFAVHLKRGPGQAEAPAGVESILLTIKDDTIYLTRHLAVLNGAVVSTP HYSPGLLIEKSDAYTKVYSRAGLTLMWNREDALMLELDTKFRNHTCGLCGDYNGLQSYSE FLSDGVLFSPLEFGNMQKINQPDVVCEDPEEEVAPASCSEHRAECERLLTAEAFADCQDL VPLEPYLRACQQDRCRCPGGDTCVCSTVAEFSRQCSHAGGRPGNWRTATLCPKTCPGNLV YLESGSPCMDTCSHLEVSSLCEEHRMDGCFCPEGTVYDDIGDSGCVPVSQCHCRLHGHLY TPGQEITNDCEQCVCNAGRWVCKDLPCPGTCALEGGSHITTFDGKTYTFHGDCYYVLAKG DHNDSYALLGELAPCGSTDKQTCLKTVVLLADKKKNVVVFKSDGSVLLNELQVNLPHVTA SFSVFRPSSYHIMVSMAIGVRLQVQLAPVMQLFVTLDQASQGQVQGLCGNFNGLEGDDFK TASGLVEATGAGFANTWKAQSSCHDKLDWLDDPCSLNIESANYAEHWCSLLKKTETPFGR CHSAVDPAEYYKRCKYDTCNCQNNEDCLCAALSSYARACTAKGVMLWGWREHVCNKDVGS CPNSQVFLYNLTTCQQTCRSLSEADSHCLEGFAPVDGCGCPDHTFLDEKGRCVPLAKCSC YHRGLYLEAGDVVVRQEERCVCRDGRLHCRQIRLIGQSCTAPKIHMDCSNLTALATSKPR ALSCQTLAAGYYHTECVSGCVCPDGLMDDGRGGCVVEKECPCVHNNDLYSSGAKIKVDCN TCTCKRGRWVCTQAVCHGTCSIYGSGHYITFDGKYYDFDGHCSYVAVQDYCGQNSSLGSF SIITENVPCGTTGVTCSKAIKIFMGRTELKLEDKHRVVIQRDEGHHVAYTTREVGQYLVV ESSTGIIVIWDKRTTVFIKLAPSYKGTVCGLCGNFDHRSNNDFTTRDHMVVSSELDFGNS WKEAPTCPDVSTNPEPCSLNPHRRSWAEKQCSILKSSVFSICHSKVDPKPFYEACVHDSC SCDTGGDCECFCSAVASYAQECTKEGACVFWRTPDLCPIFCDYYNPPHECEWHYEPCGNR SFETCRTINGIHSNISVSYLEGCYPRCPKDRPIYEEDLKKCVTADKCGCYVEDTHYPPGA SVPTEETCKSCVCTNSSQVVCRPEEGKILNQTQDGAFCYWEICGPNGTVEKHFNICSITT RPSTLTTFTTITLPTTPTTFTTTTTTTTPTSSTVLSTTPKLCCLWSDWINEDHPSSGSDD GDRETFDGVCGAPEDIECRSVKDPHLSLEQLGQKVQCDVSVGFICKNEDQFGNGPFGLCY DYKIRVNCCWPMDKCITTPSPPTTTPSPPPTSTTTLPPTTTPSPPTTTTTTPPPTTTPSP PITTTTTPPPTTTPSPPISTTTTPPPTTTPSPPTTTPSPPTTTPSPPTTTTTTPPPTTTP SPPTTTPITPPASTTTLPPTTTPSPPTTTTTTPPPTTTPSPPTTTPITPPTSTTTLPPTT TPSPPPTTTTTPPPTTTPSPPTTTTPSPPTITTTTPPPTTTPSPPTTTTTTPPPTTTPSP PTTTPITPPTSTTTLPPTTTPSPPPTTTTTPPPTTTPSPPTTTTPSPPITTTTTPPPTTT PSSPITTTPSPPTTTMTTPSPTTTPSSPITTTTTPSSTTTPSPPPTTMTTPSPTTTPSPP TTTMTTLPPTTTSSPLTTTPLPPSITPPTFSPFSTTTPTTPCVPLCNWTGWLDSGKPNFH KPGGDTELIGDVCGPGWAANISCRATMYPDVPIGQLGQTVVCDVSVGLICKNEDQKPGGV IPMAFCLNYEINVQCCECVTQPTTMTTTTTENPTPTPITTTTTVTPTPTPTSTQSTTPTP ITTTNTVTPTPTPTGTQTPTPTPITTTTTMVTPTPTITSTQTPTPTPITTTTVTPTPTPT STQRTTPTSITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPISTTT MVTPTPTPTGTQTLTPTPITTTTTVTPTPTPTGTQTPTSTPISTTTTVTPTPTPTGTQTP TLTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTKSTTPTSITTTTMVTPT PPPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTPTPITTTTTVTPTPTPTGTQTPTSTPI TTNTTVTPTPTPTGTPSTTLTPITTTTMVTPTPTPTGTQTPTSTPISTTTTVTPTPTPTG TQTPTPTPISTTTTVTPTPTPTSTQTPTTTPITTTTTVTPNPTPTGTQTPTTTPITTTTT VTPTPTPTGTQTPTTTPISTTTTVTPTPTPTGTQTPTTTAITTTTTVTPTPTPTGTQTPT STPITTTTTVTPTPTPTGTQTPTSTPISNTTTVTPTPTPTGTQTPTVTPITTTTTVTPTR TPTGTKSTTPTSITTTTMVTPTPTPTGTHTPTTTPITTTTTVTPTPTPTGTQTPTPTPIT TTTTVTPTPTPTGTQTPTSTPITTTTTVTPTPTPTGTQTPTTTPITTNTTVTPTPTPTGT QTPTTVLITTTTTMTPTPTPTSTKSTTVTPITTTTTVTPTPTPTGTQSTTLTPITTTTTV TPTPTPTGTQTPTTTPISTTTTVIPTPTPTGTQTPTSTPITTTTTVTPTPTPTGTQTPTS TPISTTTTVTPTATPTGTQTPTLTPITTTTTVTSTPTPTGTQTPTPTPITTTTTVTPTPT PTSTQTPTSTPITTTTTVTPTPTPTGTQTPTTTHITTTTTVTPTPTPTGTQAPTPTAITT TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQSPTPTAITTTTTVTPTPTPTGTQ TPTTTPITTTTTVTPTPTPTGTQSTTLTPITTTTTVTPIPTPTGTQTPTSTPITTTITVT PTPTPTGTQTPTPTPISTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTT PISTTTTVTPTPTPTGTQTPTSTPITTTTTVTPTPTPTGTQTPTPTPITTTTTVTPTPTP TGTQTPTSTPITTTTTVTPTPTPTGTQTPTPTPITTTTTVTPTPTPTGTQTPTSTPITTT TTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQSTTLTPITTTTTVTPTPTPTGTQT PTSTPITTTTTVTPTPTGTQTPTPTPISTTTTVTPTPTPTGTQTPTMTPITTTTTVTPTP TPTGTQTPTTTPISTTTTVTPTPTPTGTQTPTSTPITTTTTVTPTPTPTGTQTPTTTPIT TTTTVTPTPTPTGTQSTTLTPITTTTTVTPTPTPTGTQTPTPTPISTTTTVTPTPTPTGT QTPTTTPITTTTTVTPTPTPTGTQTPTTTPISTTTTVTPTPTPTGTQTPTSTPITTTTTV TPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQAPTPTAITTTTTVTPTPTPTGTQTPTT TPITTTTMVTPTPTPTGTQTPTSTPITTTTTVTPTPTPTGTQTPTPTPISTTTTVTPTPT PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPISTTTTVTPTPTPTGTQTPTSTPITT TTTVTPTPTPTGTQTPTPTPITTTTTVTPTPTPTGTQTPTSTPITTTTTVTPTPTPTGTQ TPTTTPITTTTTVTPTPTPTGTQSTTLTPITTTTTVTPTPTPTGTQTPTSTPITTTTTVT PTPTPTGTQTPTPTPISTTSTVTPTPTPTGTQTPTMTPITTTTTVTPTPTPTGTQTPTST PITTTTTVTPTPTPTGTQTPTMTPITTTTTVTPTPTPTGTQAPTPTAITTTTTVTPTPTP TGTQTPTTTPITTTTTVTPTPTPTGTQSTTLTPITTTTTVTPTPTPTGTQTPTPTPISTT TTVTPTPTPTGTQTPTMTPITTTTTVTPTPTPTGTQTPTTTPISTTTTVTPTPTPTGTQT PTTTPITTTTTVTPTPTPTGTQTPTTTPISTTTTVTPTPTPTGTQTPTTTPITTTTTVTP TPTPTGTQTPTTTPISTTTTVTPTPTPTGTQTPTSTPITTTTTVTPTPTPTGTQTPTTTP ITTTTTVTPTPTPTGTQAPTPTAITTTSTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPT GTQSPTPTAITTTTTVTPTPTPTGTQTPTLTPITTTTTVTPTPTPTGTQTPTPTPISTTT TVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTVLITTTTTMTPTPTPTSTKST TVTPITTTTTVTATPTPTGTQTPTMIPISTTTTVTPTPTPTTGSTGPPTHTSTAPIAELT TSNPPPESSTPQTSRSTSSPLTESTTLLSTLPPAIEMTSTAPPSTPTAPTTTSGGHTLSP PPSTTTSPPGTPTRGTTTGSSSAPTPSTVQTTTTSAWTPTPTPLSTPSIIRTTGLRPYPS SVLICCVLNDTYYAPGEEVYNGTYGDTCYFVNCSLSCTLEFYNWSCPSTPSPTPTPSKST PTPSKPSSTPSKPTPGTKPPECPDFDPPRQENETWWLCDCFMATCKYNNTVEIVKVECEP PPMPTCSNGLQPVRVEDPDGCCWHWECDCYCTGWGDPHYVTFDGLYYSYQGNCTYVLVEE ISPSVDNFGVYIDNYHCDPNDKVSCPRTLIVRHETQEVLIKTVHMMPMQVQVQVNRQAVA LPYKKYGLEVYQSGINYVVDIPELGVLVSYNGLSFSVRLPYHRFGNNTKGQCGTCTNTTS DDCILPSGEIVSNCEAAADQWLVNDPSKPHCPHSSSTTKRPAVTVPGGGKTTPHKDCTPS PLCQLIKDSLFAQCHALVPPQHYYDACVFDSCFMPGSSLECASLQAYAALCAQQNICLDW RNHTHGACLVECPSHREYQACGPAEEPTCKSSSSQQNNTVLVEGCFCPEGTMNYAPGFDV CVKTCGCVGPDNVPREFGEHFEFDCKNCVCLEGGSGIICQPKRCSQKPVTHCVEDGTYLA TEVNPADTCCNITVCKCNTSLCKEKPSVCPLGFEVKSKMVPGRCCPFYWCESKGVCVHGN AEYQPGSPVYSSKCQDCVCTDKVDNNTLLNVIACTHVPCNTSCSPGFELMEAPGECCKKC EQTHCIIKRPDNQHVILKPGDFKSDPKNNCTFFSCVKIHNQLISSVSNITCPNFDASICI PGSITFMPNGCCKTCTPRNETRVPCSTVPVTTEVSYAGCTKTVLMNHCSGSCGTFVMYSA KAQALDHSCSCCKEEKTSQREVVLSCPNGGSLTHTYTHIESCQCQDTVCGLPTGTSRRAR RSPRHLGSG
UniProt accession: Q02817

Data

Formalin-fixed, paraffin-embedded human normal colon stained with anti-MUC-2 antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells

FAQ & Publications

Frequently Asked Questions

What species does the rabbit anti-MUC-2 monoclonal antibody (ZR175) 6267 specifically react with?

This antibody specifically reacts with human MUC-2 protein.

What are the recommended storage conditions for maintaining the stability of the rabbit anti-MUC-2 monoclonal antibody?

The antibody should be stored at 2-8°C for short-term use and at -20°C for long-term storage, while avoiding repeated freeze-thaw cycles to maintain stability.

For immunohistochemistry applications using formalin-fixed, paraffin-embedded tissues, what is the suggested dilution range for the concentrated form of this antibody?

The recommended dilution for the concentrated antibody in immunohistochemistry is between 1:100 and 1:200.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.