| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | MAP-1 |
| available sizes | 100 µg |
rabbit anti-MAP-1 polyclonal antibody 6884
$376.00
Antibody summary
- Rabbit polyclonal to MAP-1
- Suitable for: ELISA
- Isotype: Whole IgG
- 100 µg
rabbit anti-MAP-1 polyclonal antibody 6884
| antibody |
|---|
| Tested applications ELISA |
| Recommended dilutions Immunoblotting: use at 1:500-1:1,000 dilution. A band of approx. 39kDa is detected. Positive control: Heart or brain tissue lysate. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. |
| Immunogen Synthetic peptide corresponding to aa 337-351 of human MAP-1. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity peptide affinty purifcation |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Modulator of apoptosis 1 (MAP-1) (MAP1) (Paraneoplastic antigen Ma4) |
| Gene names MOAP1,MOAP1 PNMA4 |
| Protein family PNMA family |
| Mass 39513Da |
| Function FUNCTION: Retrotransposon-derived protein that forms virion-like capsids (By similarity). Acts as an effector of BAX during apoptosis: enriched at outer mitochondria membrane and associates with BAX upon induction of apoptosis, facilitating BAX-dependent mitochondrial outer membrane permeabilization and apoptosis (PubMed:11060313, PubMed:16199525). Required for death receptor-dependent apoptosis (PubMed:11060313). When associated with RASSF1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation (PubMed:15949439). Also promotes autophagy: promotes phagophore closure via association with ATG8 proteins (PubMed:33783314). Acts as an inhibitor of the NFE2L2/NRF2 pathway via interaction with SQSTM1: interaction promotes dissociation of SQSTM1 inclusion bodies that sequester KEAP1, relieving inactivation of the BCR(KEAP1) complex (PubMed:33393215). {ECO:0000250|UniProtKB:Q9ERH6, ECO:0000269|PubMed:11060313, ECO:0000269|PubMed:15949439, ECO:0000269|PubMed:16199525, ECO:0000269|PubMed:33393215, ECO:0000269|PubMed:33783314}. |
| Subellular location SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:19100260}. Mitochondrion outer membrane {ECO:0000269|PubMed:16199525}. Extracellular vesicle membrane {ECO:0000250|UniProtKB:Q9ERH6}. Note=Forms virion-like extracellular vesicles that are released from cells. {ECO:0000250|UniProtKB:Q9ERH6}. |
| Tissues TISSUE SPECIFICITY: Widely expressed, with high levels in heart and brain. {ECO:0000269|PubMed:19366867}. |
| Structure SUBUNIT: Homodimer (PubMed:15949439). Under normal circumstances, held in an inactive conformation by an intramolecular interaction (PubMed:15949439). Interacts with BAX (PubMed:11060313, PubMed:16199525). Binding to RASSF1 isoform A (RASSF1A) relieves this inhibitory interaction and allows further binding to BAX (PubMed:15949439). Binds also to BCL2 and BCLX (PubMed:11060313). Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization (PubMed:15949439). Interacts with TRIM39 (PubMed:19100260). Interacts with RASSF6 (By similarity). Interacts with ATG8 proteins MAP1LC3A, MAP1LC3B and MAP1LC3C (PubMed:33783314). Does not interact with ATG8 proteins GABARAPL1, GABARAPL2 and GABARAP (PubMed:33783314). Interacts with SQSTM1; promoting dissociation of SQSTM1 inclusion bodies that sequester KEAP1 (PubMed:33393215). {ECO:0000250|UniProtKB:Q9ERH6, ECO:0000269|PubMed:11060313, ECO:0000269|PubMed:15949439, ECO:0000269|PubMed:16199525, ECO:0000269|PubMed:19100260, ECO:0000269|PubMed:33393215, ECO:0000269|PubMed:33783314}. |
| Post-translational modification PTM: Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1, this modification is inhibited by TRIM39. {ECO:0000269|PubMed:22529100}. |
| Target Relevance information above includes information from UniProt accession : Q96BY2 |
| The UniProt Consortium |
Data
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| Effect of gamma irradiation and IFN-beta on IP6K2 protein levels. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| ELISA |
Documents
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