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rabbit anti-MAP-1 polyclonal antibody 6884

$376.00

Antibody summary

  • Rabbit polyclonal to MAP-1
  • Suitable for: ELISA
  • Isotype: Whole IgG
  • 100 µg
SKU: 6884parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

MAP-1

available sizes

100 µg

Available product – rabbit anti-MAP-1 polyclonal antibody 6884

antibody
Tested applications
ELISA
Recommended dilutions
Immunoblotting: use at 1:500-1:1,000 dilution. A band of approx. 39kDa is detected.

Positive control: Heart or brain tissue lysate.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
Immunogen
Synthetic peptide corresponding to aa 337-351 of human MAP-1.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Modulator of apoptosis 1 (MAP-1) (MAP1) (Paraneoplastic antigen Ma4)
Gene names
MOAP1,MOAP1 PNMA4
Protein family
PNMA family
Mass
39513Da
Function
FUNCTION: Retrotransposon-derived protein that forms virion-like capsids (By similarity). Acts as an effector of BAX during apoptosis: enriched at outer mitochondria membrane and associates with BAX upon induction of apoptosis, facilitating BAX-dependent mitochondrial outer membrane permeabilization and apoptosis (PubMed:11060313, PubMed:16199525). Required for death receptor-dependent apoptosis (PubMed:11060313). When associated with RASSF1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation (PubMed:15949439). Also promotes autophagy: promotes phagophore closure via association with ATG8 proteins (PubMed:33783314). Acts as an inhibitor of the NFE2L2/NRF2 pathway via interaction with SQSTM1: interaction promotes dissociation of SQSTM1 inclusion bodies that sequester KEAP1, relieving inactivation of the BCR(KEAP1) complex (PubMed:33393215). {ECO:0000250|UniProtKB:Q9ERH6, ECO:0000269|PubMed:11060313, ECO:0000269|PubMed:15949439, ECO:0000269|PubMed:16199525, ECO:0000269|PubMed:33393215, ECO:0000269|PubMed:33783314}.
Subellular location
SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:19100260}. Mitochondrion outer membrane {ECO:0000269|PubMed:16199525}. Extracellular vesicle membrane {ECO:0000250|UniProtKB:Q9ERH6}. Note=Forms virion-like extracellular vesicles that are released from cells. {ECO:0000250|UniProtKB:Q9ERH6}.
Tissues
TISSUE SPECIFICITY: Widely expressed, with high levels in heart and brain. {ECO:0000269|PubMed:19366867}.
Structure
SUBUNIT: Homodimer (PubMed:15949439). Under normal circumstances, held in an inactive conformation by an intramolecular interaction (PubMed:15949439). Interacts with BAX (PubMed:11060313, PubMed:16199525). Binding to RASSF1 isoform A (RASSF1A) relieves this inhibitory interaction and allows further binding to BAX (PubMed:15949439). Binds also to BCL2 and BCLX (PubMed:11060313). Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization (PubMed:15949439). Interacts with TRIM39 (PubMed:19100260). Interacts with RASSF6 (By similarity). Interacts with ATG8 proteins MAP1LC3A, MAP1LC3B and MAP1LC3C (PubMed:33783314). Does not interact with ATG8 proteins GABARAPL1, GABARAPL2 and GABARAP (PubMed:33783314). Interacts with SQSTM1; promoting dissociation of SQSTM1 inclusion bodies that sequester KEAP1 (PubMed:33393215). {ECO:0000250|UniProtKB:Q9ERH6, ECO:0000269|PubMed:11060313, ECO:0000269|PubMed:15949439, ECO:0000269|PubMed:16199525, ECO:0000269|PubMed:19100260, ECO:0000269|PubMed:33393215, ECO:0000269|PubMed:33783314}.
Post-translational modification
PTM: Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1, this modification is inhibited by TRIM39. {ECO:0000269|PubMed:22529100}.
Target Relevance information above includes information from UniProt accession : Q96BY2
The UniProt Consortium

Data

benchmark-antibodies_anti-map-1_antibody_6884_1.jpg
Effect of gamma irradiation and IFN-beta on IP6K2 protein levels.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
ELISA

Documents

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