| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | serum |
| applications | ICC/IF, IHC, WB |
| reactivity | human, mouse, rat |
| available sizes | 1 mL, 100 µL, 25 µL |
rabbit anti-Ki67 polyclonal antibody 3609
$100.00 – $2,600.00
Antibody summary
- Rabbit polyclonal to Ki67
- Suitable for: WB, ICC/IF, IHC
- Reacts with: mouse, rat
- Isotype: IgG
- 100 µL, 25 µL, 1 mL
rabbit anti-Ki67 polyclonal antibody 3609
| target relevance |
|---|
| ki-67 is a widely used cell marker in research, particularly in the field of cell proliferation and cancer biology. It is a nuclear protein that is present during active phases of the cell cycle (G1, S, G2, and M phases) but absent in resting cells (G0 phase). As a marker of cellular proliferation, ki-67 is utilized to assess the growth fraction of a cell population and to quantify the proportion of actively dividing cells within a tissue or sample. Immunohistochemistry and immunofluorescence techniques employing antibodies targeting ki-67 are commonly employed to visualize and quantify its expression in tissues and cell cultures. Click for more on: cell markers and ki-67 |
| Protein names Proliferation marker protein Ki-67 (Antigen identified by monoclonal antibody Ki-67) (Antigen KI-67) (Antigen Ki67) |
| Gene names MKI67,MKI67 |
| Mass 358694Da |
| Function Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). |
| Subellular location Chromosome Nucleus Nucleus, nucleolus Note=Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106). |
| Structure Interacts with KIF15 (PubMed:10878014). Interacts (via the FHA domain) with NIFK (PubMed:11342549, PubMed:14659764, PubMed:16244663). Interacts with PPP1CC (PubMed:24867636). Component of a complex at least composed of ZNF335, HCFC1, CCAR2, EMSY, MKI67, RBBP5, ASH2L and WDR5; the complex is formed as a result of interactions between components of a nuclear receptor-mediated transcription complex and a histone methylation complex (PubMed:19131338). Interacts with ZNF335 (PubMed:19131338, PubMed:23178126). |
| Post-translational modification Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. |
| Biotechnology Widely used as a marker to assess cell proliferation, as it is detected in the nucleus of proliferating cells only (PubMed:6339421, PubMed:21960707). In cancer research field for example, MKI67 is the most widely used marker for comparing proliferation between tumor samples (PubMed:21960707, PubMed:26680267). |
| Target Relevance information above includes information from UniProt accession: P46013 |
| The UniProt Consortium |
Data
 |
| Immunofluorescent analysis of HeLa cells stained with rabbit pAb to Ki67 3609, dilution 1:5,000 in red, and mouse monoclonal antibody to fibrillarin, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. The Ki67 protein accumulates in and around the nucleoli of interphase cells such as those on the right, and the nucleoli are revealed by the fibrillarin antibody. In contrast, cells in the quiescent G0 state such as those on the left are Ki67 negative but fibrillarin positive. |
 |
| Chromogenic immunostaining of a 4%PFA fixed paraffin embedded rat testes section with rabbit pAb to rodent Ki67, 3609, dilution 1:2,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In testes, 2990-Ro strongly labels the nuclei of spermatogenic cells. This antibody was not tested for staining on NBF fixed tissues. Both rat and mouse tissues stain effectively with 3609. |
 |
| Western blot analysis of equal amounts of cell lysates using rabbit pAb to Ki67 3609, dilution 1:10,000, (green): [1] protein standard (red), [2] rapidly growing HeLa cell cultures, [3] rapidly growing HEK293 cell cultures. Strong double bands larger than the 250kDa standard correspond to full length 345kDa and 395kDa Ki67 isoforms, while smaller proteolytic fragments of these isoforms are also invariably detected on the blot. |
Publications
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| 27362226 | Ki-67 acts as a biological surfactant to disperse mitotic chromosomes. | Sara Cuylen, Claudia Blaukopf, Antonio Z Politi, Thomas Müller-Reichert, Beate Neumann, Ina Poser, Jan Ellenberg, Anthony A Hyman, Daniel W Gerlich | Nature 535:308-12 |
| 23235554 | Retrospective analysis of risk factors for central nervous system metastases in operable breast cancer: effects of biologic subtype and Ki67 overexpression on survival. | Mikiya Ishihara, Hirofumi Mukai, Shunji Nagai, Masakatsu Onozawa, Keiji Nihei, Toshiyuki Shimada, Noriaki Wada | Oncology 84:135-40 |
| 22452635 | Low endoglin vascular density and Ki67 index in Gleason score 6 tumours may identify prostate cancer patients suitable for surveillance. | Andreas Josefsson, Pernilla Wikström, Lars Egevad, Torvald Granfors, Lars Karlberg, Pär Stattin, Anders Bergh | Scand J Urol Nephrol 46:247-57 |
| 20152769 | Ki67 in breast cancer: prognostic and predictive potential. | Rinat Yerushalmi, Ryan Woods, Peter M Ravdin, Malcolm M Hayes, Karen A Gelmon | Lancet Oncol 11:174-83 |
| 19436038 | Ki67 index, HER2 status, and prognosis of patients with luminal B breast cancer. | Maggie C U Cheang, Stephen K Chia, David Voduc, Dongxia Gao, Samuel Leung, Jacqueline Snider, Mark Watson, Sherri Davies, Philip S Bernard, Joel S Parker, Charles M Perou, Matthew J Ellis, Torsten O Nielsen | J Natl Cancer Inst 101:736-50 |
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| # | SDS | Certificate | |
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