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rabbit anti-Ki67 polyclonal antibody 2990

$100.00$2,600.00

Antibody summary

  • Rabbit polyclonal to Ki67
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: human, mouse, rat
  • Isotype: IgG
  • 100 µL, 25 µL, 1 mL
SKU: 2990parent Categories: , Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

serum

applications

ICC/IF, IHC, WB

available sizes

1 mL, 100 µL, 25 µL

Available product – rabbit anti-Ki67 polyclonal antibody 2990

antibody
Database link:
human P46013
mouse E9PVX6
rat Q5RJM0
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
WB: 1:5000-10000 IF 1:2000-5000, IHC 1:1000
Immunogen
Recombinant human construct containing amino acids 1,111-1,490 expressed in and purified from E. coli
Size and concentration
25, 100, 1000µL and serum
Form
liquid
Storage Instructions
2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer
serum, 0.04% NaN3 added
Purity
serum
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Ki67 is a widely used cell marker in research, particularly in the field of cell proliferation and cancer biology. It is a nuclear protein that is present during active phases of the cell cycle (G1, S, G2, and M phases) but absent in resting cells (G0 phase). As a marker of cellular proliferation, Ki67 is utilized to assess the growth fraction of a cell population and to quantify the proportion of actively dividing cells within a tissue or sample. Immunohistochemistry and immunofluorescence techniques employing antibodies targeting Ki67 are commonly employed to visualize and quantify its expression in tissues and cell cultures.

Click for more on: cell markers and ki67
Protein names
Proliferation marker protein Ki-67 (Antigen identified by monoclonal antibody Ki-67) (Antigen KI-67) (Antigen Ki67)
Gene names
MKI67,MKI67
Mass
358694Da
Function
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Subellular location
Chromosome Nucleus Nucleus, nucleolus Note=Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
Structure
Interacts with KIF15 (PubMed:10878014). Interacts (via the FHA domain) with NIFK (PubMed:11342549, PubMed:14659764, PubMed:16244663). Interacts with PPP1CC (PubMed:24867636). Component of a complex at least composed of ZNF335, HCFC1, CCAR2, EMSY, MKI67, RBBP5, ASH2L and WDR5; the complex is formed as a result of interactions between components of a nuclear receptor-mediated transcription complex and a histone methylation complex (PubMed:19131338). Interacts with ZNF335 (PubMed:19131338, PubMed:23178126).
Post-translational modification
Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
Biotechnology
Widely used as a marker to assess cell proliferation, as it is detected in the nucleus of proliferating cells only (PubMed:6339421, PubMed:21960707). In cancer research field for example, MKI67 is the most widely used marker for comparing proliferation between tumor samples (PubMed:21960707, PubMed:26680267).
Target Relevance information above includes information from UniProt accession : P46013
The UniProt Consortium

Data

ICC/IF-image-rabbit-anti-Ki67-polyclonal-antibody-2990
Immunofluorescent analysis of HeLa cells stained with rabbit pAb to Ki67 2990, dilution 1:5,000 in red, and mouse monoclonal antibody to fibrillarin, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. The Ki67 protein accumulates in and around the nucleoli of interphase cells such as those on the right, and the nucleoli are revealed by the fibrillarin antibody. In contrast, cells in the quiescent G0 state such as those on the left are Ki67 negative but fibrillarin positive.
IHC-image-rabbit-anti-Ki67-polyclonal-antibody-2990
A section of human breast tissue including both normal and cancer cells. The cancer cells divide rapidly and heavily express Ki67 and so stain strongly with the 2990 antibody. Formalin fixed and paraffin embedded section. Mouse select image for larger view.
IHC-image-rabbit-anti-Ki67-polyclonal-antibody-2990
Chromogenic immunostaining of a 4%PFA fixed paraffin embedded rat testes section with rabbit pAb to Ki67, 2990, dilution 1:5,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In testes, the 2990 antibody strongly labels the nuclei of spermatogenic cells. This antibody performs well in 4%PFA or NBF fixed tissues. Both rodent and human tissues stain effectively with 2990. Mouse select image for larger view
IHC-image-rabbit-anti-Ki67-polyclonal-antibody-2990
Chromogenic immunostaining of a NBF fixed paraffin embedded human midbrain section with metastatic carcinoma of the meninges using rabbit pAb to Ki67, 2990, dilution 1:5,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In this image, the 2990 antibody strongly labels the nuclei of the actively dividing cancer cells. Mouse select image for larger view.
ICC/IF-image-rabbit-anti-Ki67-polyclonal-antibody-2990
High magnification confocal image of adult mouse hippocampus dentate region stained with 2990, 1:2,000 dilution, in red and mouse monoclonal antibody to FOX3/NeuN, 1:2,000 in green. Blue is Hoechst dye staining of DNA. Top left is DNA, top right FOX3/NeuN, bottom left Ki67 and bottom right all three merged. Dividing cells are very rare in adult animals, but one can be seen in the center of the image. Chromosomes can be seen in blue and their Ki67 coating can be seen in red. The dividing cell is FOX3/NeuN negative and so is presumably a glial cell. Mouse select image for larger view.
ICC/IF-image-rabbit-anti-Ki67-polyclonal-antibody-2990
Mouse NIH-3T3 cells stained with 2990 1:5,000 in red and mouse mAb to beta-tubulin, 1:2,000 in green. The Ki67 strongly stains the nuclei of dividing cells, but not quiescent cells. DNA is shown in blue using Hoeschst dye. Mouse select image for larger view.
WB-image-rabbit-anti-Ki67-polyclonal-antibody-2990
Western blot analysis of equal amounts of cell lysates using rabbit pAb to Ki67 2990, dilution 1:10,000, (green): [1] protein standard (red), [2] rapidly growing HeLa cell cultures, [3] rapidly growing HEK293 cell cultures. Strong double bands larger than the 250kDa standard correspond to full length 345kDa and 395kDa Ki67 isoforms, while smaller proteolytic fragments of these isoforms are also invariably detected on the blot.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation
36661357The role of a ciliary GTPase in the regulation of neuronal maturation of olfactory sensory neuronsHabif JC, Xie C, de Celis C, Ukhanov K, Green WW, Moretta JC, Zhang L, Campbell RJ, Martens JR.Development. 2023 Jan 15;150(2):dev201116. doi: 10.1242/dev.201116. Epub 2023 Jan 19.
35129203Fibroblast activation protein-alpha knockdown suppresses prostate cancer cell invasion and proliferationAn J, Hou D, Wang L, Wang L, Yang Y, Wang H.Histol Histopathol. 2022 Jun;37(6):597-607. doi: 10.14670/HH-18-430. Epub 2022 Feb 7.
34842137Altered temporal sequence of transcriptional regulators in the generation of human cerebellar granule cellsBehesti H, Kocabas A, Buchholz DE, Carroll TS, Hatten ME.Elife. 2021 Nov 29;10:e67074. doi: 10.7554/eLife.67074.
31386828A neutral lipid-enriched diet improves myelination and alleviates peripheral nerve pathology in neuropathic miceZhou Y, Bazick H, Miles JR, Fethiere AI, Salihi MOA, Fazio S, Tavori H, Notterpek L.Exp Neurol. 2019 Nov;321:113031. doi: 10.1016/j.expneurol.2019.113031. Epub 2019 Aug 3.
29246495Elevated Peripheral Myelin Protein 22, Reduced Mitotic Potential, and Proteasome Impairment in Dermal Fibroblasts from Charcot-Marie-Tooth Disease Type 1A PatientsLee S, Bazick H, Chittoor-Vinod V, Al Salihi MO, Xia G, Notterpek L.Am J Pathol. 2018 Mar;188(3):728-738. doi: 10.1016/j.ajpath.2017.10.021. Epub 2017 Dec 12.
27362226Ki-67 acts as a biological surfactant to disperse mitotic chromosomesCuylen S, Blaukopf C, Politi AZ, Müller-Reichert T, Neumann B, Poser I, Ellenberg J, Hyman AA, Gerlich DW.Nature. 2016 Jul 14;535(7611):308-12. doi: 10.1038/nature18610. Epub 2016 Jun 29.
23235554Retrospective analysis of risk factors for central nervous system metastases in operable breast cancer: effects of biologic subtype and Ki67 overexpression on survivalIshihara M, Mukai H, Nagai S, Onozawa M, Nihei K, Shimada T, Wada N.Oncology. 2013;84(3):135-40. doi: 10.1159/000345321. Epub 2012 Dec 11.
22452635Low endoglin vascular density and Ki67 index in Gleason score 6 tumours may identify prostate cancer patients suitable for surveillanceJosefsson A, Wikström P, Egevad L, Granfors T, Karlberg L, Stattin P, Bergh A.Scand J Urol Nephrol. 2012 Aug;46(4):247-57. doi: 10.3109/00365599.2012.669791. Epub 2012 Mar 27.
20152769Ki67 in breast cancer: prognostic and predictive potentialYerushalmi R, Woods R, Ravdin PM, Hayes MM, Gelmon KA.Lancet Oncol. 2010 Feb;11(2):174-83. doi: 10.1016/S1470-2045(09)70262-1.
19436038Ki67 index, HER2 status, and prognosis of patients with luminal B breast cancerCheang MC, Chia SK, Voduc D, Gao D, Leung S, Snider J, Watson M, Davies S, Bernard PS, Parker JS, Perou CM, Ellis MJ, Nielsen TO.J Natl Cancer Inst. 2009 May 20;101(10):736-50. doi: 10.1093/jnci/djp082. Epub 2009 May 12.
19141773Multi-institutional validation of the predictive value of Ki-67 labeling index in patients with urinary bladder cancerMargulis V, Lotan Y, Karakiewicz PI, Fradet Y, Ashfaq R, Capitanio U, Montorsi F, Bastian PJ, Nielsen ME, Müller SC, Rigaud J, Heukamp LC, Netto G, Lerner SP, Sagalowsky AI, Shariat SF.J Natl Cancer Inst. 2009 Jan 21;101(2):114-9. doi: 10.1093/jnci/djn451. Epub 2009 Jan 13.
7911472The expression of proliferation-dependent antigens during the lifespan of normal and progeroid human fibroblasts in cultureKill IR, Faragher RG, Lawrence K, Shall S.J Cell Sci. 1994 Feb;107 ( Pt 2):571-9. doi: 10.1242/jcs.107.2.571.
6339421Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferationGerdes J, Schwab U, Lemke H, Stein H.Int J Cancer. 1983 Jan 15;31(1):13-20. doi: 10.1002/ijc.2910310104.

Protocols

relevant to this product
Western blot
IHC
ICC

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