rabbit anti-HO1 polyclonal antibody 1008

$100.00 – $2,600.00

Antibody summary

Rabbit polyclonal to HO-1 (Heme oxygenase-1)
Suitable for: WB,IP
Reacts with: Hu
Isotype: IgG
100 µg, 25 µg, 1 mg

SKU: 1008parent Categories: antibody, cell marker, oxidative stress Tags: HO-1 (Heme oxygenase-1), polyclonal, rabbit

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	IP, WB
reactivity	HO-1 (Heme oxygenase-1), polyclonal, rabbit
available sizes	1 mg, 100 µg, 25 µg

rabbit anti- ho1 polyclonal antibody 1008

antibody
Database link: human P09601
Tested applications WB,IP
Recommended dilutions Western Blot 1:2,000 - 1:10,000, Immunoprecipitation 2 - 10 %micro;g/mg lysate
Immunogen The epitope recognized by A303-662A maps to a region between residue 238 and 288 of human Heme Oxygenase (decycling) 1 using the numbering given in entry NP_002124.1 (GeneID 3162).
Size and concentration 100µL and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Heme oxygenase 1 (HO-1) (EC 1.14.14.18) [Cleaved into: Heme oxygenase 1 soluble form]
Gene names HMOX1,HMOX1 HO HO1
Protein family Heme oxygenase family
Mass 32819Da
Function FUNCTION: [Heme oxygenase 1]: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:11121422, PubMed:19556236, PubMed:7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707). {ECO:0000269\|PubMed:11121422, ECO:0000269\|PubMed:19556236, ECO:0000269\|PubMed:7703255, ECO:0000303\|PubMed:20055707}.; FUNCTION: [Heme oxygenase 1]: (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy. {ECO:0000269\|PubMed:35239449}.; FUNCTION: [Heme oxygenase 1 soluble form]: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. {ECO:0000269\|PubMed:7703255}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=heme b + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = biliverdin IXalpha + CO + Fe(2+) + H(+) + 3 H2O + 3 oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:21764, Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17245, ChEBI:CHEBI:29033, ChEBI:CHEBI:57618, ChEBI:CHEBI:57991, ChEBI:CHEBI:58210, ChEBI:CHEBI:60344; EC=1.14.14.18; Evidence={ECO:0000269\|PubMed:11121422, ECO:0000269\|PubMed:19556236, ECO:0000269\|PubMed:7703255}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:21765; Evidence={ECO:0000305\|PubMed:7703255};
Subellular location SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269\|PubMed:19556236, ECO:0000269\|PubMed:22419571, ECO:0000269\|PubMed:27184847}; Single-pass type IV membrane protein {ECO:0000255}; Cytoplasmic side {ECO:0000269\|PubMed:22419571}.
Tissues TISSUE SPECIFICITY: Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). {ECO:0000269\|PubMed:20855888}.
Structure SUBUNIT: (Microbial infection) Interacts with SARS-CoV-2 ORF3A protein; the interaction promotes ORF3A-induced autophagy but is unlikely to be involved in ORF3A-mediated induction of reticulophagy. {ECO:0000269\|PubMed:35239449}.; SUBUNIT: Homodimer and higher order homooligomer (PubMed:19556236). Oligomerization is crucial for its stability and function in the endoplasmic reticulum (PubMed:19556236). Interacts with FLVCR2; this interaction is potentiated in the presence of heme. {ECO:0000250\|UniProtKB:P14901, ECO:0000269\|PubMed:19556236}.
Post-translational modification PTM: A soluble form arises by proteolytic removal of the membrane anchor. {ECO:0000305\|PubMed:7703255}.
Domain DOMAIN: Th
Target Relevance information above includes information from UniProt accession : P09601
The UniProt Consortium

Data

WB-image-anti-HO-1 (Heme oxygenase-1) antibody-1008_1

Detection of human HO-1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HO-1 antibody #1008 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. HO-1 was also immunoprecipitated by rabbit anti-HO-1 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.