| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | IP, WB |
| reactivity | human |
| available sizes | 100 µg, 25 µg |
rabbit anti-HO1 polyclonal antibody 1008
$150.00 – $2,600.00
Antibody summary
- Rabbit polyclonal to HO-1 (Heme oxygenase-1)
- Suitable for: WB,IP
- Reacts with: Hu
- Isotype: IgG
- 100 µg, 25 µg
rabbit anti- ho1 polyclonal antibody 1008
| antibody |
|---|
| Database link: human P09601 |
| Tested applications WB,IP |
| Recommended dilutions Western Blot 1:2,000 - 1:10,000, Immunoprecipitation 2 - 10 µg/mg lysate |
| Immunogen The epitope recognized by A303-662A maps to a region between residue 238 and 288 of human Heme Oxygenase (decycling) 1 using the numbering given in entry NP_002124.1 (GeneID 3162). |
| Size and concentration 100µL and 1 mg/mL |
| Form liquid |
| Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles. |
| Storage buffer Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Heme oxygenase 1 (HO-1) (EC 1.14.14.18) [Cleaved into: Heme oxygenase 1 soluble form] |
| Gene names HMOX1,HMOX1 HO HO1 |
| Protein family Heme oxygenase family |
| Mass 32819Da |
| Function [Heme oxygenase 1]: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:11121422, PubMed:19556236, PubMed:7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707).; [Heme oxygenase 1]: (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.; [Heme oxygenase 1 soluble form]: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. |
| Catalytic activity CATALYTIC ACTIVITY: Reaction=heme b + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = biliverdin IXalpha + CO + Fe(2+) + H(+) + 3 H2O + 3 oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:21764, Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17245, ChEBI:CHEBI:29033, ChEBI:CHEBI:57618, ChEBI:CHEBI:57991, ChEBI:CHEBI:58210, ChEBI:CHEBI:60344; EC=1.14.14.18; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:21765; Evidence=; |
| Subellular location Endoplasmic reticulum membrane ; Single-pass type IV membrane protein ; Cytoplasmic side . |
| Tissues Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). |
| Post-translational modification A soluble form arises by proteolytic removal of the membrane anchor. |
| Domain Th |
| Involvement in disease DISEASE: Heme oxygenase 1 deficiency (HMOX1D) [MIM:614034]: A disease characterized by impaired stress hematopoiesis, resulting in marked erythrocyte fragmentation and intravascular hemolysis, coagulation abnormalities, endothelial damage, and iron deposition in renal and hepatic tissues. Clinical features include persistent hemolytic anemia, asplenia, nephritis, generalized erythematous rash, growth retardation and hepatomegaly. Note=The disease is caused by variants affecting the gene represented in this entry. |
| Target Relevance information above includes information from UniProt accession: P09601 |
| The UniProt Consortium |
 |
| Detection of human HO-1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HO-1 antibody #1008 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. HO-1 was also immunoprecipitated by rabbit anti-HO-1 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B). |
Publications
| pmid | title | authors | citation |
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| We haven't added any publications to our database yet. | |||
| relevant to this product |
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| Western blot |
| # | SDS | Certificate | |
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| Please enter your product and batch number here to retrieve product datasheet, SDS, and QC information. | |||
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