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rabbit anti-FOXO3a polyclonal antibody 1039

$100.00$2,600.00

Antibody summary

  • Rabbit polyclonal to FOXO (Forkhead box O) Transcription Factor 3a
  • Suitable for: IP,WB
  • Reacts with: Hu, Ms
  • Isotype: IgG
  • 100 µg, 25 µg, 1 mg
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

IP, WB

reactivity

FOXO (Forkhead box O) Transcription Factors, monoclonal, rabbit

available sizes

1 mg, 100 µg, 25 µg

rabbit anti- foxo3a polyclonal antibody 1039

antibody
Database link:
human O43524
mouse Q9WVH4
Tested applications
IP,WB
Recommended dilutions
Immunoprecipitation (IP) 6 %micro;l/mg lysate, Western Blot (WB) 1:1000 - 1:5000, IHC 1:200 - 1:1000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Immunogen
Between 275 and 325
Size and concentration
100µL and 1 mg/mL
Form
liquid
Storage Instructions
Store at 2-8°C. Expires 1 year from date of receipt.
Storage buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity
affinity purified
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit monolconal - Isotype Control
No information
target relevance

Data

WB-image-anti-FOXO (Forkhead box O) Transcription Factor 3a antibody-10
Detection of human FOXO3a by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MCF-7 cells. Antibodies: Affinity purified rabbit anti-FOXO3a antibody (#1039) used for IP at 6 µg/mg lysate. FOXO3a was also immunoprecipitated by rabbit anti-FOXO3a antibody. For blotting immunoprecipitated FOXO3a was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
WB-image-anti-FOXO (Forkhead box O) Transcription Factor 3a antibody-10
Detection of human and mouse FOXO3a by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-FOXO3a antibody (#1039) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
IHC-image-anti-FOXO (Forkhead box O) Transcription Factor 3a antibody-1
Detection of human and mouse FOXO3a by immunohistochemistry. Sample: FFPE sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-FOXO3a (#1039) used at a dilution of 1:500 (2µg/ml). Detection: DAB

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot

Documents

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