rabbit anti-Calreticulin monoclonal antibody 9015

$409.00

Antibody summary

Rabbit monoclonal to Calreticulin
Suitable for: WB, ICC/IF, IHC
Reacts with: human, mouse, rat
Isotype: IgG
100 µg

SKU: 9015 Categories: antibody, cell marker Tag: Calreticulin

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
available sizes	100 µg

rabbit anti-Calreticulin monoclonal antibody 9015

antibody
Database link: human P27797 mouse P14211 rat P18418
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:1000-1:2000 IF/ICC and IHC: 1:1000
Immunogen Synthetic peptides VESGSLEDDWDFLPPKKI corresponding to amino acids 191-208 of human calreticulin, including the LC3 interacting region or LIR.
Size and concentration 100µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal IgG - Isotype Control

target relevance
Calreticulin, an abundant protein found in the endoplasmic reticulum (ER), serves both as an essential organelle marker and a cell marker. Within the ER, calreticulin plays a critical role in calcium homeostasis and protein folding, ensuring proper maturation and quality control of newly synthesized proteins. Its presence and function in the ER make calreticulin a valuable organelle marker, aiding in the identification and localization of this dynamic and essential cellular compartment. Additionally, calreticulin has emerged as a prominent cell marker due to its distinct expression patterns in various cell types and physiological conditions. By detecting and measuring the levels of calreticulin on the cell surface, researchers can identify and isolate specific cell populations, such as stem cells or cancer cells. The ability to use calreticulin as both an organelle and cell marker offers valuable insights into cellular processes and opens up new avenues for studying cell biology, disease mechanisms, and developing targeted therapies. Click for more on: cell markers and Calreticulin
Protein names Calreticulin (CRP55) (Calregulin) (Endoplasmic reticulum resident protein 60) (ERp60) (HACBP) (grp60)
Gene names CALR,CALR CRTC
Protein family Calreticulin family
Mass 48142Da
Function Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed:7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed:11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).
Subellular location Endoplasmic reticulum lumen Cytoplasm, cytosol Secreted, extracellular space, extracellular matrix Cell surface Sarcoplasmic reticulum lumen Cytoplasmic vesicle, secretory vesicle, Cortical granule Cytolytic granule Note=Also found in cell surface (T cells), cytosol and extracellular matrix (PubMed:10358038). During oocyte maturation and after parthenogenetic activation accumulates in cortical granules. In pronuclear and early cleaved embryos localizes weakly to cytoplasm around nucleus and more strongly in the region near the cortex (By similarity). In cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation (By similarity).
Structure Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 and SPACA9 (By similarity). Interacts with TRIM21 (PubMed:8666824). Interacts with NR3C1 (PubMed:11149926). Interacts with PPIB (PubMed:20801878). Interacts (via P-domain) with PDIA5 (PubMed:23614004). Interacts with GABARAP (PubMed:19154346). Interacts with HLA-E-B2M and HLA-G-B2M complexes (PubMed:9427624, PubMed:9640257). Interacts with HLA-F (PubMed:10605026). Interacts with CLCC1 (PubMed:30157172).
Involvement in disease Note=CALR somatic mutations are frequently found in myeloproliferative neoplasms lacking JAK2 or MPL mutations. Myeloproliferative neoplasms are chronic myeloid cancers characterized by overproduction of mature blood cells, and may evolve into acute myeloid leukemia. In addition to chronic myeloid leukemia with the BCR-ABL fusion gene, the three most common myeloproliferative neoplasms are essential thrombocythemia, polycythemia vera, and myelofibrosis.
Target Relevance information above includes information from UniProt accession: P27797
The UniProt Consortium

benchmarkantibodies.com/wp-content/uploads/2024/03/9015_1.jpg

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.