rabbit anti-ATR polyclonal antibody 8335

$518.00

Antibody summary

Rabbit polyclonal to ATR
Suitable for: WB,IP
Isotype: Whole IgG
100 µg

SKU: 8335parent Category: antibody Tags: ATR, polyclonal, rabbit

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	ATR
available sizes	100 µg

rabbit anti-ATR polyclonal antibody 8335

antibody

Tested applications WB,IP
Recommended dilutions Western Blot: 0.033 - 0.067 ug/ml.
Immunogen Synthetic peptide representing a portion of the protein encoded within exon 29 (LocusLink ID 545).
Size and concentration 100µg and lot specific
Form liquid
Storage Instructions Store at 2 - 8°C. Antibody is stable at 2 - 8°C for 1 year.
Storage buffer Tris-citrate/phosphate buffer, pH 7 to 8 contai
Purity immunogen affinty purifcation
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Serine/threonine-protein kinase ATR (EC 2.7.11.1) (Ataxia telangiectasia and Rad3-related protein) (FRAP-related protein 1)
Gene names ATR,ATR FRP1
Protein family PI3/PI4-kinase family, ATM subfamily
Mass 301367Da
Function FUNCTION: Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor (PubMed:10597277, PubMed:10608806, PubMed:10859164, PubMed:11721054, PubMed:12791985, PubMed:12814551, PubMed:14657349, PubMed:14729973, PubMed:14742437, PubMed:15210935, PubMed:15496423, PubMed:16260606, PubMed:21144835, PubMed:27723717, PubMed:27723720, PubMed:33848395, PubMed:9427750, PubMed:9636169, PubMed:21777809). Recognizes the substrate consensus sequence [ST]-Q (PubMed:10597277, PubMed:10608806, PubMed:10859164, PubMed:11721054, PubMed:12791985, PubMed:12814551, PubMed:14657349, PubMed:14729973, PubMed:14742437, PubMed:15210935, PubMed:15496423, PubMed:16260606, PubMed:21144835, PubMed:27723717, PubMed:27723720, PubMed:33848395, PubMed:9427750, PubMed:9636169). Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis (PubMed:9925639, PubMed:11114888, PubMed:11418864, PubMed:11865061, PubMed:21777809). Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism (PubMed:11673449). Required for FANCD2 ubiquitination (PubMed:15314022). Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication (PubMed:12526805). Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex (By similarity). {ECO:0000250\|UniProtKB:Q9JKK8, ECO:0000269\|PubMed:10597277, ECO:0000269\|PubMed:10608806, ECO:0000269\|PubMed:10859164, ECO:0000269\|PubMed:11114888, ECO:0000269\|PubMed:11418864, ECO:0000269\|PubMed:11673449, ECO:0000269\|PubMed:11721054, ECO:0000269\|PubMed:11865061, ECO:0000269\|PubMed:12526805, ECO:0000269\|PubMed:12791985, ECO:0000269\|PubMed:12814551, ECO:0000269\|PubMed:14657349, ECO:0000269\|PubMed:14729973, ECO:0000269\|PubMed:14742437, ECO:0000269\|PubMed:15210935, ECO:0000269\|PubMed:15314022, ECO:0000269\|PubMed:15496423, ECO:0000269\|PubMed:16260606, ECO:0000269\|PubMed:21144835, ECO:0000269\|PubMed:21777809, ECO:0000269\|PubMed:27723717, ECO:0000269\|PubMed:27723720, ECO:0000269\|PubMed:33848395, ECO:0000269\|PubMed:9427750, ECO:0000269\|PubMed:9636169, ECO:0000269\|PubMed:9925639}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1; Evidence={ECO:0000269\|PubMed:27723717, ECO:0000269\|PubMed:27723720}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17990; Evidence={ECO:0000269\|PubMed:27723717, ECO:0000269\|PubMed:27723720}; CATALYTIC ACTIVITY: Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1; Evidence={ECO:0000269\|PubMed:21705319, ECO:0000269\|PubMed:21777809, ECO:0000269\|PubMed:27723717, ECO:0000269\|PubMed:27723720}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:46609; Evidence={ECO:0000269\|PubMed:21705319, ECO:0000269\|PubMed:21777809, ECO:0000269\|PubMed:27723717, ECO:0000269\|PubMed:27723720};
Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000269\|PubMed:11114888, ECO:0000269\|PubMed:11721054, ECO:0000269\|PubMed:11865061, ECO:0000269\|PubMed:12814551, ECO:0000269\|PubMed:14871897, ECO:0000269\|PubMed:16260606, ECO:0000269\|PubMed:18283122, ECO:0000269\|PubMed:8843195}. Chromosome {ECO:0000269\|PubMed:11673449}. Note=Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
Tissues TISSUE SPECIFICITY: Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary. {ECO:0000269\|PubMed:11470508, ECO:0000269\|PubMed:8610130, ECO:0000269\|PubMed:8843195}.
Structure SUBUNIT: Forms a heterodimer with ATRIP, forming the ATR-ATRIP complex (PubMed:11721054, PubMed:15758953, PubMed:14729973, PubMed:12791985, PubMed:21777809). Present in a complex containing ATRIP and RPA-coated single-stranded DNA (PubMed:14729973, PubMed:12791985). Binds to DNA, and to UV-damaged DNA with higher affinity (PubMed:12791985). Interacts with MSH2 and HDAC2 (PubMed:14657349). Present in a complex containing CHD4 and HDAC2 (PubMed:10545197). Interacts with EEF1E1, the interaction is enhanced by UV irradiation (PubMed:15680327). Interacts with CLSPN and CEP164 (PubMed:18283122, PubMed:12766152). Interacts with TELO2 and TTI1 (PubMed:20801936, PubMed:20427287, PubMed:20810650). Interacts with BCR-ABL after genotoxic stress (PubMed:15050919). Interacts with UHRF2; this interaction promotes ATR activation (PubMed:33848395). Interacts (when phosphorylated) with TOPBP1; interaction takes place when ATR is autophosphorylated at Thr-1989, leading to ATR activation by TOPBP1 (PubMed:21705319, PubMed:21777809). {ECO:0000269\|PubMed:10545197, ECO:0000269\|PubMed:11721054, ECO:0000269\|PubMed:12766152, ECO:0000269\|PubMed:12791985, ECO:0000269\|PubMed:14657349, ECO:0000269\|PubMed:14729973, ECO:0000269\|PubMed:15050919, ECO:0000269\|PubMed:15680327, ECO:0000269\|PubMed:15758953, ECO:0000269\|PubMed:18283122, ECO:0000269\|PubMed:20427287, ECO:0000269\|PubMed:20801936, ECO:0000269\|PubMed:20810650, ECO:0000269\|PubMed:21705319, ECO:0000269\|PubMed:21777809, ECO:0000269\|PubMed:33848395}.
Post-translational modification PTM: Phosphorylated (PubMed:21144835). Autophosphorylation at Thr-1989 in response to DNA damage promotes interaction with TOPBP1 and activation of ATR (PubMed:21705319, PubMed:21777809). {ECO:0000269\|PubMed:21144835, ECO:0000269\|PubMed:21705319, ECO:0000269\|PubMed:21777809}.
Target Relevance information above includes information from UniProt accession : Q13535
The UniProt Consortium

Data

Detection of human ATR by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATR antibody 8335 (lot 8335-4) used for IP at 3 µg per reaction. ATR was also immunoprecipitated by rabbit anti-ATR antibody 13016. For blotting immunoprecipitated ATR, 8335 was used at 1 µg /ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human ATR by western blot. Samples: Whole cell lysate (15 µg ) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody 8335 (lot 8335-4) used for WB at 0.4 µg /ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmid	title	authors	citation

Protocols

relevant to this product
Western blot

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