IHC image mouse anti Ubiquitin monoclonal antibody Ubi1 737

IHC image mouse anti Ubiquitin monoclonal antibody Ubi1 1737

mouse anti-Ubiquitin monoclonal antibody (Ubi1) 1737

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to Ubiquitin
Suitable for: WB, ICC/IF, IHC
Reacts with: all
Isotype: IgG1
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 1737parent Categories: antibody, cell marker Tag: Ubiquitin

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	modified proteins
available sizes	1 mg, 100 µg, 25 µg

mouse anti-Ubiquitin monoclonal antibody (Ubi1) 1737

antibody
Database link: Ubiquitin Sequence - MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:1000-1:2000 ICC/IF and IHC: 1:2000
Immunogen Purified bovine erythrocyte ubiquitin coupled to KLH with glutaraldehyde
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance

Ubiquitin plays a fundamental role in cell biology as a key regulator of protein degradation and cellular processes.

More on: cell markers and ubiquitin

Biotechnology
Ubiquitin, a small protein found in nearly all eukaryotic cells, plays a fundamental role in regulating various cellular processes. Its primary function is to mark proteins for degradation through the ubiquitin-proteasome system, a highly controlled and selective process known as ubiquitination. When a protein is destined for degradation or clearance, multiple ubiquitin molecules are covalently attached to specific lysine residues on the target protein, forming a polyubiquitin chain. This ubiquitin chain acts as a signal, directing the tagged protein to the proteasome, a large multi-subunit protein complex responsible for protein degradation. Once delivered to the proteasome, the ubiquitinated protein is unfolded and processed, and its constituent amino acids are recycled for the synthesis of new proteins. Apart from its role in protein degradation, ubiquitin also participates in various non-proteolytic functions, such as DNA repair, endocytosis, and signal transduction, where monoubiquitination or other ubiquitin modifications can serve as regulatory signals. The precise and intricate control of ubiquitin-mediated processes ensures proper cellular homeostasis, protein turnover, and the maintenance of critical cellular functions.

Data

Formalin fixed paraffin embedded section of cerebral cortex of an Alzheimer patient processed with 1737 using HRP/ DAB, giving a brown signal. Also stained with haemotoxylin in blue. A typical flame shaped tangle is seen in a pyramidal neuron in the center and is surrounded by some dystrophic neurites, also strongly ubiquitin positive. Both are commonly seen in cortical and hippocampal Alzheimer brain sections and are typical for this disease, but are rare or absent in healthy brain.

Chromogenic immunostaining of a NBF fixed paraffin embedded human hippocampus section from an Alzheimer's Disease case. Mouse mAb to ubiquitin, 1737, dilution 1:2,000, was detected in DAB (brown) following the ImmPress method with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. The 1737 antibody strongly labels flame shapped tangles in pyramidal neurons and dystrophic neurites nuclei characteristic of Alzheimer's disease. This antibody performs well in testing with 4% PFA or standard NBF fixed human and rat tissues.

Blots probed with 1737 of mono and K48 linked polyubiquitin (Boston Biotech, lane 1), monoubiquitin only (2), and 100µg total wet weight of homogenates of rat cerebellum, cortex and brain stem respectively (lanes 3-5). Material was run out on 20% SDS-PAGE and transferred electrophoretically to PVDF. 1737 binds both mono and polyubiquitin and detects monoubiquitin in cell and tissue lysates.

Western blot analysis of HEK293 cell lysates using mouse mAb to ubiquitin, 1737, dilution 1:1,000 in green. [1] protein standard (red), [2] cells maintained in normal medium, [3] cells treated with 10µM of proteasome inhibitor lactacystin (Lc) for 16hrs. Lysed cells were electrophoresed on 4-20% SDS-PAGE, and transferred to PVDF membranes. The smear detected above the 200kDa standard represent accumulation of ubiquitinated proteins in proteasome inhibitor-Lc treated cells. The prominent band at 8kDa corresponds to monoubiquitin. The same blot was probed with rabbit pAb to HSP60, 2417, dilution 1:5,000 in red, used as a loading control.

Publications

pmid	title	authors	citation
36378690	The autophagic protein p62 is a target of reactive aldehydes in human and murine cholestatic liver disease.	Colin T Shearn, Aimee L Anderson, Michael W Devereux, David J Orlicky, Cole Michel, Dennis R Petersen, Colin G Miller, Sanjiv Harpavat, Edward E Schmidt, Ronald J Sokol	PLoS One 17:e0276879
36121476	Stress-inducible phosphoprotein 1 (HOP/STI1/STIP1) regulates the accumulation and toxicity of ?-synuclein in vivo.	Rachel E Lackie, Aline S de Miranda, Mei Peng Lim, Vladislav Novikov, Nimrod Madrer, Nadun C Karunatilleke, Benjamin S Rutledge, Stephanie Tullo, Anne Brickenden, Matthew E R Maitland, David Greenberg, Daniel Gallino, Wen Luo, Anoosha Attaran, Irina Shlaifer, Esther Del Cid Pellitero, Caroline Schild-Poulter, Thomas M Durcan, Edward A Fon, Martin Duennwald, Flavio H Beraldo, M Mallar Chakravarty, Timothy J Bussey, Lisa M Saksida, Hermona Soreq, Wing-Yiu Choy, Vania F Prado, Marco A M Prado	Acta Neuropathol 144:881-910
35388108	Melatonin reduces the endoplasmic reticulum stress and polyubiquitinated protein accumulation induced by repeated anesthesia exposure in Caenorhabditis elegans.	Hyun-Jung Shin, Bon-Wook Koo, Jiwon Yoon, Heeyeon Kim, Sang-Hwan Do, Hyo-Seok Na	Sci Rep 12:5783
34819832	Suppressor of Cytokine Signaling 2 Regulates Retinal Pigment Epithelium Metabolism by Enhancing Autophagy.	Xi-Yuan Liu, Rui Lu, Jing Chen, Jie Wang, Hong-Mei Qian, Gang Chen, Rong-Han Wu, Zai-Long Chi	Front Neurosci 15:738022
34646768	OTUB2 Facilitates Tumorigenesis of Gastric Cancer Through Promoting KDM1A-Mediated Stem Cell-Like Properties.	Guangming Liu, Wei Guo, Junjie Qin, Zhiliang Lin	Front Oncol 11:711735

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.