Western blot mouse anti tsg101 monoclonal antibody 4a10 4454

ELISA mouse anti tsg101 monoclonal antibody 4a10 4454

IHC mouse anti tsg101 monoclonal antibody 4a10 4454

FC mouse anti tsg101 monoclonal antibody 4a10 4454

mouse anti-TSG101 monoclonal antibody (4A10) 4454

$503.00

Antibody summary

Mouse monoclonal to TSG101
Suitable for: WB,ICC/IF,IHC-P,FACS,IP,ELISA,EM,IHC,IHC
Isotype: IgG1
100 µl

SKU: 4454parent Category: antibody Tags: monoclonal, mouse, TSG101

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	TSG101
available sizes	100 µL

mouse anti-TSG101 monoclonal antibody (4A10) 4454

antibody

Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions ELISA: use at 1-5ug/ml. Immunoblotting: use at a 1:500-1:3,000 dilution. A band of 46kDa is detected. Detection of TSG101 protein in (A) NIH-3T3 cell lysate, (B) JC cell lysate, and (C) BCL-1 cell lysate with #3352 diluted 1:500. Endusers should determine optimal antibody concentra
Immunogen Recombinant protein corresponding to aa 167-374 of TSG101 protein
Size and concentration 100µL and lot specific
Form liquid
Storage Instructions This product is stable for at least one (1) year if stored at -20°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Storage buffer PBS, pH 7.2.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance
Protein names Tumor susceptibility gene 101 protein (ESCRT-I complex subunit TSG101)
Gene names TSG101,TSG101
Protein family Ubiquitin-conjugating enzyme family, UEV subfamily
Mass 43944Da
Function FUNCTION: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426). {ECO:0000269\|PubMed:11916981, ECO:0000269\|PubMed:17556548, ECO:0000269\|PubMed:17853893, ECO:0000269\|PubMed:21070952, ECO:0000269\|PubMed:21757351, ECO:0000269\|PubMed:22315426, ECO:0000269\|PubMed:22660413}.
Subellular location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269\|PubMed:17853893}. Early endosome membrane {ECO:0000269\|PubMed:23166352}; Peripheral membrane protein {ECO:0000305\|PubMed:23166352}; Cytoplasmic side {ECO:0000305\|PubMed:23166352}. Late endosome membrane {ECO:0000269\|PubMed:11916981, ECO:0000269\|PubMed:17229889}; Peripheral membrane protein. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269\|PubMed:17853893}. Midbody, Midbody ring {ECO:0000269\|PubMed:17556548, ECO:0000269\|PubMed:17853893}. Nucleus {ECO:0000269\|PubMed:17229889}. Note=Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Nuclear localization is cell cycle-dependent. Interaction with CEP55 is required for localization to the midbody during cytokinesis. {ECO:0000269\|PubMed:17556548, ECO:0000269\|PubMed:17853893}.
Tissues TISSUE SPECIFICITY: Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
Structure SUBUNIT: Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry (PubMed:18005716). Interacts with VPS37A, VPS37B and VPS37C (PubMed:15218037, PubMed:15509564). Interacts with DMAP1 (PubMed:10888872). Interacts with ubiquitin (PubMed:11595185). Interacts with stathmin, GMCL and AATF (By similarity). Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoichiometry (PubMed:21757351). Interacts with HGS; the interaction mediates the association with the ESCRT-0 complex. Interacts with GGA1 and GGA3 (PubMed:15143060, PubMed:15039775). Interacts (via UEV domain) with PDCD6IP/AIP1 (PubMed:14505570, PubMed:14519844). Interacts with VPS28, SNF8 and VPS36 (PubMed:14505570). Self-associates (PubMed:14505570, PubMed:14519844). Interacts with MVB12A; the association appears to be mediated by the TSG101-VPS37 binary subcomplex. Interacts with VPS37D. Interacts with LRSAM1. Interacts with CEP55; the interaction is required for cytokinesis but not for viral budding (PubMed:17853893). Interacts with PDCD6 (PubMed:18256029). Interacts with LITAF (PubMed:23166352). Interacts with MGRN1 (PubMed:17229889). Interacts with ARRDC1; recruits TSG101 to the plasma membrane (PubMed:21191027, PubMed:22315426). {ECO:0000250, ECO:0000250\|UniProtKB:Q6IRE4, ECO:0000269\|PubMed:10888872, ECO:0000269\|PubMed:14505570, ECO:0000269\|PubMed:15039775, ECO:0000269\|PubMed:15053872, ECO:0000269\|PubMed:15143060, ECO:0000269\|PubMed:15218037, ECO:0000269\|PubMed:15509564, ECO:0000269\|PubMed:15858022, ECO:0000269\|PubMed:17229889, ECO:0000269\|PubMed:17556548, ECO:0000269\|PubMed:17853893, ECO:0000269\|PubMed:18005716, ECO:0000269\|PubMed:18256029, ECO:0000269\|PubMed:19520058, ECO:0000269\|PubMed:19703557, ECO:0000269\|PubMed:21191027, ECO:0000269\|PubMed:21757351, ECO:0000269\|PubMed:22315426, ECO:0000269\|PubMed:23166352}.; SUBUNIT: (Microbial infection) Interacts with HIV-1 p6. {ECO:0000269\|PubMed:11427703, ECO:0000269\|PubMed:11595185, ECO:0000269\|PubMed:12900394, ECO:0000269\|PubMed:21070952, ECO:0000269\|PubMed:21643473}.; SUBUNIT: (Microbial infection) Interacts with human spumavirus Gag. {ECO:0000269\|PubMed:15858022}.; SUBUNIT: (Microbial infection) Interacts with HTLV-1 Gag. {ECO:0000269\|PubMed:14581525}.; SUBUNIT: (Microbial infection) Interacts with Ebola virus VP40. {ECO:0000269\|PubMed:12559917}.; SUBUNIT: (Microbial infection) Interacts with EIAV p9; the interaction has been shown in vitro. {ECO:0000269\|PubMed:14519844}.; SUBUNIT: (Microbial infection) Interacts with Lassa virus protein Z. {ECO:0000269\|PubMed:16571837}.; SUBUNIT: (Microbial infection) Interacts with hepatitis E virus protein ORF3. {ECO:0000269\|PubMed:21068219}.
Post-translational modification PTM: Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1. {ECO:0000269\|PubMed:15256501, ECO:0000269\|PubMed:17229889, ECO:0000269\|PubMed:19703557}.
Target Relevance information above includes information from UniProt accession : Q99816
The UniProt Consortium

Data

TSG101 antibody [4A10] detects TSG101 protein by western blot analysis.
A. 30 µg NIH-3T3 whole cell lysate/extract
B. 30 µg JC whole cell lysate/extract
C. 30 µg BCL-1 whole cell lysate/extract
10% SDS-PAGE
TSG101 antibody [4A10] (4454) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

ELISA detection of TSG101 using for capture at a concentration of 5 µg/mL and 4454 for detection at a concentration of 1.5 µg/mL.

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (4454) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TSG101 stained by TSG101 antibody [4A10] (4454) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
TSG101 stained by TSG101 antibody [4A10] (4454) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

TSG101 antibody [4A10] (4454) detects TSG101 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: TSG101 antibody [4A10] (4454) dilution: 1:25.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (4454) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (4454) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
TSG101 stained by TSG101 antibody [4A10] (4454) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

There are 642 publications in our database for this antibody or clone. Here are the latest 5, for more click below.

pmid	title	authors	citation
38339765	Bioengineered small extracellular vesicles deliver multiple SARS-CoV-2 antigenic fragments and drive a broad immunological response	Jackson HK, Long HM, Yam-Puc JC, Palmulli R, Haigh TA, Gerber PP, Lee JS, Matheson NJ, Young L, Trowsdale J, Lo M, Taylor GS, Thaventhiran JE, Edgar JR.	J Extracell Vesicles. 2024 Feb;13(2):e12412. doi: 10.1002/jev2.12412.
38339765	Bioengineered small extracellular vesicles deliver multiple SARS-CoV-2 antigenic fragments and drive a broad immunological response	Jackson HK, Long HM, Yam-Puc JC, Palmulli R, Haigh TA, Gerber PP, Lee JS, Matheson NJ, Young L, Trowsdale J, Lo M, Taylor GS, Thaventhiran JE, Edgar JR.	J Extracell Vesicles. 2024 Feb;13(2):e12412. doi: 10.1002/jev2.12412.
38338867	CD99 Modulates the Proteomic Landscape of Ewing Sarcoma Cells and Related Extracellular Vesicles	De Feo A, Manfredi M, Mancarella C, Maqueda JJ, De Giorgis V, Pignochino Y, Sciandra M, Cristalli C, Donadelli M, Scotlandi K.	Int J Mol Sci. 2024 Jan 27;25(3):1588. doi: 10.3390/ijms25031588.
38338867	CD99 Modulates the Proteomic Landscape of Ewing Sarcoma Cells and Related Extracellular Vesicles	De Feo A, Manfredi M, Mancarella C, Maqueda JJ, De Giorgis V, Pignochino Y, Sciandra M, Cristalli C, Donadelli M, Scotlandi K.	Int J Mol Sci. 2024 Jan 27;25(3):1588. doi: 10.3390/ijms25031588.
38225453	Deep proteomic analysis of obstetric antiphospholipid syndrome by DIA-MS of extracellular vesicle enriched fractions	Tian W, Shi D, Zhang Y, Wang H, Tang H, Han Z, Wong CCL, Cui L, Zheng J, Chen Y.	Commun Biol. 2024 Jan 15;7(1):99. doi: 10.1038/s42003-024-05789-3.