Immunohistochemistry (IHC) : Human Ewing’s sarcoma stained with anti-NKX2.2 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells.

mouse anti-NKX2.2 monoclonal antibody (ZM14) 6289

Price range: $160.00 through $528.00

Antibody summary

Mouse monoclonal to NKX2.2
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: Ewing’s sarcoma
Visualization: Nuclear
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

available sizes

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SKU: 6289parent Category: antibody Tags: anatomy, NKX2.2, Soft Tissue Pathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-NKX2.2 monoclonal antibody ZM14 6289

antibody
Database link: human O95096
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Ewing's sarcoma
Immunogen Human recombinant NKX2.2 protein fragment (aa 1-119)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names Homeobox protein Nkx-2.2 (Homeobox protein NK-2 homolog B)
Gene names NKX2-2,NKX2-2 NKX2.2 NKX2B
Protein family NK-2 homeobox family
Mass 30133Da
Function FUNCTION: Transcriptional activator involved in the development of insulin-producting beta cells in the endocrine pancreas (By similarity). May also be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Binds to elements within the NEUROD1 promoter (By similarity). {ECO:0000250\|UniProtKB:P42586}.
Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00108}.
Structure SUBUNIT: Interacts with OLIG2. {ECO:0000250}.
Domain DOMAIN: The homeodomain is essential for interaction with OLIG2. {ECO:0000250}.
Target Relevance information above includes information from UniProt accession: O95096
The UniProt Consortium

Data

Human Ewing'ss sarcoma stained with anti-NKX2.2 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.