Immunohistochemistry (IHC) : Human mesothelioma stained with anti-Mesothelin antibody using peroxidase-conjugate and DAB chromogen. Note membranous staining of tumor cells.

mouse anti-Mesothelin monoclonal antibody (ZM25) 6253

Price range: $160.00 through $528.00

Antibody summary

Mouse monoclonal to Mesothelin
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG2b
Control: Mesothelioma
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6253parent Category: antibody Tags: anatomy, Lung/Mediastinum Pathology, Mesothelin

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2b
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-Mesothelin monoclonal antibody ZM25 6253

antibody
Database link: human Q13421
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Mesothelioma
Immunogen Recombinant human mesothelin (MSLN) protein fragment (around aa 273-407)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG2b
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG2b - Isotype Control

target relevance
Protein names Mesothelin (CAK1 antigen) (Pre-pro-megakaryocyte-potentiating factor) [Cleaved into: Megakaryocyte-potentiating factor (MPF); Mesothelin, cleaved form]
Gene names MSLN,MSLN MPF
Protein family Mesothelin family
Mass 68986Da
Function FUNCTION: Membrane-anchored forms may play a role in cellular adhesion.; FUNCTION: Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Subellular location SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus.; SUBCELLULAR LOCATION: [Megakaryocyte-potentiating factor]: Secreted.; SUBCELLULAR LOCATION: [Isoform 3]: Secreted.
Tissues TISSUE SPECIFICITY: Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). {ECO:0000269\|PubMed:16857795, ECO:0000269\|PubMed:7665620}.
Structure SUBUNIT: Interacts with MUC16. {ECO:0000269\|PubMed:14676194, ECO:0000269\|PubMed:22787150}.
Post-translational modification PTM: Both MPF and the cleaved form of mesothelin are N-glycosylated. {ECO:0000269\|PubMed:16857795, ECO:0000269\|PubMed:8288629, ECO:0000269\|PubMed:8552591}.; PTM: Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. {ECO:0000269\|PubMed:8552591}.
Involvement in disease DISEASE: Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. {ECO:0000269\|PubMed:15897581}.
Target Relevance information above includes information from UniProt accession: Q13421
The UniProt Consortium

Data

Human mesothelioma stained with anti-Mesothelin antibody using peroxidase-conjugate and DAB chromogen. Note membranous staining of tumor cells.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.