ICC/IF image mouse anti MAP2 monoclonal antibody 5H11 1367

IHC image mouse anti MAP2 monoclonal antibody 5H11 1367

mouse anti-MAP2 monoclonal antibody (5H11) 1367

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to MAP2
Suitable for: WB, ICC/IF, IHC
Reacts with: human, mouse, rat
Isotype: IgG2b
100 µg, 25 µg, 1 mg

SKU: 1367parent Categories: antibody, cell marker Tag: MAP2

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2b
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	human, mouse, rat
available sizes	1 mg, 100 µg, 25 µg

mouse anti-MAP2 monoclonal antibody (5H11) 1367

antibody
Database link: human P11137 mouse P20357 rat P15146
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:10000 IF/ICC and IHC: 1:1000
Immunogen Full length purified bovine protein, epitope mapped to projection domain of human sequence, amino acids 1057-1588 using EnCor product Prot-r-MAP2-P3
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG2b
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG2b - Isotype Control

target relevance
As a key component of the cytoskeleton in neurons, MAP2 plays a critical role in regulating microtubule dynamics and stabilizing neuronal processes such as dendrites and axons. Its abundant presence in these structures makes it an essential factor in establishing and maintaining the intricate neuronal morphology required for proper cell function and connectivity in the nervous system. Researchers have focused on studying MAP2 to gain deeper insights into neuronal development, synaptic plasticity, and neurodegenerative diseases, as its expression and phosphorylation are closely associated with synaptic maturation and neuronal plasticity. As a cell marker, MAP2 serves as an invaluable marker for identifying neuronal differentiation and assessing neuronal damage or degeneration. This antibody recognizes MAP2 in neuronal cells and can be used as a cell marker antibody when used with a suitable secondary antibody. Click for more on: cell markers and MAP2
Protein names Microtubule-associated protein 2 (MAP-2)
Gene names MAP2,MAP2
Mass 199526Da
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Subellular location Cytoplasm, cytoskeleton Cell projection, dendrite
Structure Interacts with KNDC1 (via KIND2); the interaction enhances MAP2 phosphorylation and localizes KNDC1 to dendrites. Interacts with DPYSL5 (PubMed:33894126).
Post-translational modification Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).
Target Relevance information above includes information from UniProt accession: P11137
The UniProt Consortium

Immunofluorescent analysis of rat hippocampus section stained with mouse mAb to MAP2, 1367, dilution 1:5,000 in green, and costained with rabbit pAb to alpha-internexin dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with above antibodies. The 1367 antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, and the alpha-internexin antibody selectively stains axons and dendrites of neuronal cells.

Chromogenic Immunostaining of a formalin fixed paraffin embedded human brain cortex section with mouse mAb to MAP2, 1367, dilution 1:5,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. 367 strongly labels neurons, neuronal projections, and synapses. This antibody performs well in testing with 4% PFA fixed or standard NBF fixed rat and human tissue but does not stain long term NBF fixed material effectively. Due to its strong staining profile and ability to stain human tissue, 1367 is our recommended clone for MAP2 immunostaining.

Chromogenic immunostaining of a 4% PFA fixed paraffin embedded rat cerebellum section with mouse mAb to MAP2, 1367, dilution 1:5,000, detected in DAB (brown) following the ImmPress method with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In the cerebellum, 1367 strongly labels neurons, neuronal projections, and synapses. This antibody performs well in testing with 4% PFA fixed or standard NBF fixed rat and human tissue but does not stain long term NBF fixed material effectively. Due to its strong staining profile and ability to stain human tissue, 1367 is our recommended clone for MAP2 immunostaining.

Immunofluorescent analysis of a rat cerebellum section stained with mouse mAb to MAP2, 1367, dilution 1:5,000 in green, and costained with rabbit pAb to alpha-internexin dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies. The 367 antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, particularly in granular cell and molecular layers of the cerebellum, while the alpha-internexin antibody selectively stains axons and dendrites of neuronal cells.

WB-image-mouse-anti-MAP2-monoclonal-antibody-5H11-1367

Western blot analysis of different tissue lysates using mouse mAb to MAP2A/B, 1367, dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat whole brain, [2] embryonic (E20) rat brain, [4] adult rat spinal cord, and [5] adult mouse brain lysate. A band at about 280 kDa corresponds to full length MAP2a and MAP2B protein. MAP2A/B is expressed heavily in adult brain particularly in cortical regions, but is a more minor component of spinal cord and almost absent from the embryonic brain sample. Note that since the epitope for this antibody in within the projection domain found only in MAP2A and MAP2B, and so the antibody does not bind to the lower molecular weight MAP2C and MAP2D isoforms which lack this region.

Publications

pmid	title	authors	citation
22623668	An ankyrinG-binding motif is necessary and sufficient for targeting Nav1.6 sodium channels to axon initial segments and nodes of Ranvier.	Andreas Gasser, Tammy Szu-Yu Ho, Xiaoyang Cheng, Kae-Jiun Chang, Stephen G Waxman, Matthew N Rasband, Sulayman D Dib-Hajj	J Neurosci 32:7232-43
18988710	Gliotypic neural stem cells transiently adopt tumorigenic properties during normal differentiation.	Noah M Walton, Gregory E Snyder, Donghyun Park, Firas Kobeissy, Bjorn Scheffler, Dennis A Steindler	Stem Cells 27:280-9
16832065	Temporally restricted substrate interactions direct fate and specification of neural precursors derived from embryonic stem cells.	A Katrin Goetz, Bjorn Scheffler, Huan-Xin Chen, Shanshan Wang, Oleg Suslov, Hui Xiang, Oliver Brüstle, Steve N Roper, Dennis A Steindler	Proc Natl Acad Sci U S A 103:11063-8
16817858	Differential modulation of sodium channel Na(v)1.6 by two members of the fibroblast growth factor homologous factor 2 subfamily.	Anthony M Rush, Ellen K Wittmack, Lynda Tyrrell, Joel A Black, Sulayman D Dib-Hajj, Stephen G Waxman	Eur J Neurosci 23:2551-62
16487970	Neuronal vulnerability in transgenic mice expressing an inducible dominant-negative FGF receptor.	Felix P Eckenstein, Toby McGovern, Drew Kern, Jason Deignan	Exp Neurol 198:338-49

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.