Skip to content

mouse anti-MAP2 monoclonal antibody (5H11) 1367

$100.00$2,600.00

Antibody summary

  • Mouse monoclonal to MAP2
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: human, mouse, rat
  • Isotype: IgG2b
  • 100 µg, 25 µg, 1 mg
SKU: 1367parent Categories: , Tags: ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG2b

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, IHC, WB

available sizes

1 mg, 100 µg, 25 µg

Available product – mouse anti-MAP2 monoclonal antibody (5H11) 1367

antibody
Database link:
human P11137
mouse P20357
rat P15146
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
WB: 1:10000 IF/ICC and IHC: 1:1000
Immunogen
Full length purified bovine protein, epitope mapped to projection domain of human sequence, amino acids 1057-1588 using EnCor product Prot-r-MAP2-P3
Size and concentration
25, 100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS, 50% glycerol, 0.04% NaN3
Purity
affinity purified
Clonality
monoclonal
Isotype
IgG2b
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG2b - Isotype Control
target relevance
As a key component of the cytoskeleton in neurons, MAP2 plays a critical role in regulating microtubule dynamics and stabilizing neuronal processes such as dendrites and axons. Its abundant presence in these structures makes it an essential factor in establishing and maintaining the intricate neuronal morphology required for proper cell function and connectivity in the nervous system. Researchers have focused on studying MAP2 to gain deeper insights into neuronal development, synaptic plasticity, and neurodegenerative diseases, as its expression and phosphorylation are closely associated with synaptic maturation and neuronal plasticity.

As a cell marker, MAP2 serves as an invaluable marker for identifying neuronal differentiation and assessing neuronal damage or degeneration. This antibody recognizes MAP2 in neuronal cells and can be used as a cell marker antibody when used with a suitable secondary antibody.

Click for more on: cell markers and MAP2
Protein names
Microtubule-associated protein 2 (MAP-2)
Gene names
MAP2,MAP2
Mass
199526Da
Function
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Subellular location
Cytoplasm, cytoskeleton Cell projection, dendrite
Structure
Interacts with KNDC1 (via KIND2); the interaction enhances MAP2 phosphorylation and localizes KNDC1 to dendrites. Interacts with DPYSL5 (PubMed:33894126).
Post-translational modification
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).
Target Relevance information above includes information from UniProt accession : P11137
The UniProt Consortium

Data

ICC/IF-image-mouse-anti-MAP2-monoclonal-antibody-5H11-1367
Immunofluorescent analysis of rat hippocampus section stained with mouse mAb to MAP2, 1367, dilution 1:5,000 in green, and costained with rabbit pAb to alpha-internexin dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with above antibodies. The 1367 antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, and the alpha-internexin antibody selectively stains axons and dendrites of neuronal cells.
IHC-image-mouse-anti-MAP2-monoclonal-antibody-5H11-1367
Chromogenic Immunostaining of a formalin fixed paraffin embedded human brain cortex section with mouse mAb to MAP2, 1367, dilution 1:5,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. 367 strongly labels neurons, neuronal projections, and synapses. This antibody performs well in testing with 4% PFA fixed or standard NBF fixed rat and human tissue but does not stain long term NBF fixed material effectively. Due to its strong staining profile and ability to stain human tissue, 1367 is our recommended clone for MAP2 immunostaining. Mouse select image for larger view.
IHC-image-mouse-anti-MAP2-monoclonal-antibody-5H11-1367
Chromogenic immunostaining of a 4% PFA fixed paraffin embedded rat cerebellum section with mouse mAb to MAP2, 1367, dilution 1:5,000, detected in DAB (brown) following the ImmPress method with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In the cerebellum, 1367 strongly labels neurons, neuronal projections, and synapses. This antibody performs well in testing with 4% PFA fixed or standard NBF fixed rat and human tissue but does not stain long term NBF fixed material effectively. Due to its strong staining profile and ability to stain human tissue, 1367 is our recommended clone for MAP2 immunostaining. Mouse select image for larger view.
ICC/IF-image-mouse-anti-MAP2-monoclonal-antibody-5H11-1367
Immunofluorescent analysis of a rat cerebellum section stained with mouse mAb to MAP2, 1367, dilution 1:5,000 in green, and costained with rabbit pAb to alpha-internexin dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies. The 367 antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, particularly in granular cell and molecular layers of the cerebellum, while the alpha-internexin antibody selectively stains axons and dendrites of neuronal cells. Mouse select image for larger view.
WB-image-mouse-anti-MAP2-monoclonal-antibody-5H11-1367
Western blot analysis of different tissue lysates using mouse mAb to MAP2A/B, 1367, dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat whole brain, [2] embryonic (E20) rat brain, [4] adult rat spinal cord, and [5] adult mouse brain lysate. A band at about 280 kDa corresponds to full length MAP2a and MAP2B protein. MAP2A/B is expressed heavily in adult brain particularly in cortical regions, but is a more minor component of spinal cord and almost absent from the embryonic brain sample. Note that since the epitope for this antibody in within the projection domain found only in MAP2A and MAP2B, and so the antibody does not bind to the lower molecular weight MAP2C and MAP2D isoforms which lack this region.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation
22623668An ankyrinG-binding motif is necessary and sufficient for targeting Nav1.6 sodium channels to axon initial segments and nodes of RanvierGasser A, Ho TS, Cheng X, Chang KJ, Waxman SG, Rasband MN, Dib-Hajj SD.J Neurosci. 2012 May 23;32(21):7232-43. doi: 10.1523/JNEUROSCI.5434-11.2012.
18988710Gliotypic neural stem cells transiently adopt tumorigenic properties during normal differentiationWalton NM, Snyder GE, Park D, Kobeissy F, Scheffler B, Steindler DA.Stem Cells. 2009 Feb;27(2):280-9. doi: 10.1634/stemcells.2008-0842.
16832065Temporally restricted substrate interactions direct fate and specification of neural precursors derived from embryonic stem cellsGoetz AK, Scheffler B, Chen HX, Wang S, Suslov O, Xiang H, Brüstle O, Roper SN, Steindler DA.Proc Natl Acad Sci U S A. 2006 Jul 18;103(29):11063-8. doi: 10.1073/pnas.0510926103. Epub 2006 Jul 10.
16817858Differential modulation of sodium channel Na(v)1.6 by two members of the fibroblast growth factor homologous factor 2 subfamilyRush AM, Wittmack EK, Tyrrell L, Black JA, Dib-Hajj SD, Waxman SG.Eur J Neurosci. 2006 May;23(10):2551-62. doi: 10.1111/j.1460-9568.2006.04789.x.
16487970Neuronal vulnerability in transgenic mice expressing an inducible dominant-negative FGF receptorEckenstein FP, McGovern T, Kern D, Deignan J.Exp Neurol. 2006 Apr;198(2):338-49. doi: 10.1016/j.expneurol.2005.12.020. Epub 2006 Feb 17.
15642108The MAP2/Tau family of microtubule-associated proteinsDehmelt L, Halpain S.Genome Biol. 2005;6(1):204. doi: 10.1186/gb-2004-6-1-204. Epub 2004 Dec 23.
12368089Intrinsically unstructured proteinsTompa P.Trends Biochem Sci. 2002 Oct;27(10):527-33. doi: 10.1016/s0968-0004(02)02169-2.
6120944A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins (MAPs)Vallee RB.J Cell Biol. 1982 Feb;92(2):435-42. doi: 10.1083/jcb.92.2.435.
2469170Immature and mature variants of MAP2 and tau proteins and neuronal plasticityNunez J.Trends Neurosci. 1988 Nov;11(11):477-9. doi: 10.1016/0166-2236(88)90004-5.

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

#
No results found

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.