mouse anti-LAMP1 monoclonal antibody (5H6) 2181

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to LAMP1
Suitable for: WB, ICC/IF, IHC
Reacts with: human
Isotype: IgG1
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 2181parent Categories: antibody, cell marker Tag: LAMP1

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	human
available sizes	1 mg, 100 µg, 25 µg

mouse anti-LAMP1 monoclonal antibody (5H6) 2181

antibody
Database link: human P11279
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:10000 IF/ICC and IHC: 1:2000
Immunogen Amino acids 32-350 of the human LAMP1 precursor sequence in NP_005552.3 purified from E. coli
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance
Lysosomal-associated membrane protein 1 (LAMP1) serves as an essential cell marker in various fields of research, particularly in cell biology and immunology. LAMP1 is a glycoprotein localized to lysosomal membranes and late endosomes, playing a critical role in maintaining lysosomal integrity and facilitating endocytic processes. Due to its specific subcellular localization, LAMP1 is used as a marker to identify lysosomes and late endosomes in different cell types and tissues. Immunofluorescence and immunohistochemistry techniques, employing antibodies targeting LAMP1, enable researchers to visualize and study lysosomal compartments and their dynamics. Furthermore, LAMP1 is valuable in investigating lysosomal-related diseases, autophagy, and vesicle trafficking. In immunology, LAMP1 has gained importance as a marker for dendritic cell maturation and activation, aiding in the study of antigen presentation and immune response regulation. Click for more on: cell markers and Lamp1
Protein names Lysosome-associated membrane glycoprotein 1 (LAMP-1) (Lysosome-associated membrane protein 1) (CD107 antigen-like family member A) (CD antigen CD107a)
Gene names LAMP1,LAMP1
Protein family LAMP family
Mass 44882Da
Function FUNCTION: Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed:37390818). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed:37390818). Also plays an important role in NK-cells cytotoxicity (PubMed:2022921, PubMed:23632890). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed:23632890). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed:23847195). Presents carbohydrate ligands to selectins (PubMed:7685349). {ECO:0000269\|PubMed:2022921, ECO:0000269\|PubMed:23632890, ECO:0000269\|PubMed:23847195, ECO:0000269\|PubMed:37390818, ECO:0000269\|PubMed:7685349}.; FUNCTION: (Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed:24970085, PubMed:25972533, PubMed:27605678, PubMed:28448640). Also promotes fusion of the virus with host membrane in less acidic endosomes (PubMed:29295909). {ECO:0000269\|PubMed:24970085, ECO:0000269\|PubMed:25972533, ECO:0000269\|PubMed:27605678, ECO:0000269\|PubMed:28448640, ECO:0000269\|PubMed:29295909}.; FUNCTION: (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F. {ECO:0000269\|PubMed:32295904}.
Subellular location SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000269\|PubMed:16176980, ECO:0000269\|PubMed:17897319}; Single-pass type I membrane protein {ECO:0000255}. Endosome membrane {ECO:0000269\|PubMed:16176980}; Single-pass type I membrane protein {ECO:0000255}. Late endosome membrane {ECO:0000269\|PubMed:16176980}; Single-pass type I membrane protein {ECO:0000255}. Cell membrane {ECO:0000269\|PubMed:2022921, ECO:0000269\|PubMed:23847195}; Single-pass type I membrane protein {ECO:0000255}. Cytolytic granule membrane {ECO:0000269\|PubMed:2022921, ECO:0000269\|PubMed:24088571}; Single-pass type I membrane protein {ECO:0000255}. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane (By similarity). Colocalizes with OSBPL1A at the late endosome (PubMed:16176980). {ECO:0000250\|UniProtKB:P05300, ECO:0000269\|PubMed:16176980, ECO:0000269\|PubMed:17897319}.
Structure SUBUNIT: Interacts with ABCB9; this interaction strongly stabilizes ABCB9 and protects ABCB9 against lysosomal degradation (PubMed:22641697). Interacts with FURIN (PubMed:32295904). Interacts with TMEM175; inhibiting the proton channel activity of TMEM175 (PubMed:37390818). {ECO:0000269\|PubMed:22641697, ECO:0000269\|PubMed:32295904, ECO:0000269\|PubMed:37390818}.; SUBUNIT: (Microbial infection) Interacts with Lassa virus protein glycoprotein. {ECO:0000269\|PubMed:24970085, ECO:0000269\|PubMed:25972533, ECO:0000269\|PubMed:27605678, ECO:0000269\|PubMed:28448640}.; SUBUNIT: (Microbial infection) Interacts with mumps virus protein F; this interaction promotes protein F cleavage by FURIN. {ECO:0000269\|PubMed:32295904}.
Post-translational modification PTM: O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. {ECO:0000269\|PubMed:12754519, ECO:0000269\|PubMed:16335952, ECO:0000269\|PubMed:19159218, ECO:0000269\|PubMed:8323299}.; PTM: (Microbial infection) The glycosylation of Asn-76 is essential for Lassa virus entry into cells. {ECO:0000269\|PubMed:24970085}.
Target Relevance information above includes information from UniProt accession: P11279
The UniProt Consortium

Data

Immunofluorescent analysis of HeLa cells stained with mouse mAb to LAMP1, 2181, dilution 1:500 in red, and costained with chicken pAb to vimentin, 6886, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The cells were treated with 50µM of chloroquine, an inhibitor of autophagy, for 16 hours prior to staining. The 2181 antibody reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the 6886 antibody specifically labels the intermediate filament network in these cells.

WB-image-mouse-anti-LAMP1-monoclonal-antibody-5H6-2181

Western blot analysis of different cell lysates using mouse mAb to LAMP1, 2181 dilution 1:10,000 in green. Cells were maintained under normal conditions (Ct), or treated with 50µM of chloroquine (CQ), an inhibitor of autophagy, for 24 hours: [1] protein standard (red), [2] HeLa Ct, [3] HeLa+CQ, [4] NIH-3T3 Ct, and [5] NIH-3T3+CQ. The smeared band between 75-120kDa corresponds to variably glycosylated forms of the LAMP1 protein detected only in the human cells, this antibody does not recognize the rodent LAMP1 homologue. The same blot was probed with chicken pAb to heat shock 60 protein (HSP60), 416, dilution 1:20,000. The HSP60 antibody reveals a band with apparent molecular weight of 60kDa in all preparations.

Publications

pmid	title	authors	citation
8647888	The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane.	J Rohrer, A Schweizer, D Russell, S Kornfeld	J Cell Biol 132:565-76
3308906	Translocation and clustering of endosomes and lysosomes depends on microtubules.	R Matteoni, T E Kreis	J Cell Biol 105:1253-65
3174652	Derived protein sequence, oligosaccharides, and membrane insertion of the 120-kDa lysosomal membrane glycoprotein (lgp120): identification of a highly conserved family of lysosomal membrane glycoproteins.	C L Howe, B L Granger, M Hull, S A Green, C A Gabel, A Helenius, I Mellman	Proc Natl Acad Sci U S A 85:7577-81

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.