mouse anti-Ki67 monoclonal antibody (6B4) 1820

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to Ki67
Suitable for: WB, ICC/IF
Reacts with: human
Isotype: IgG1
100 µg, 25 µg, 1 mg

SKU: 1820parent Categories: antibody, cell marker Tag: Ki-67

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	human
available sizes	1 mg, 100 µg, 25 µg

mouse anti-Ki67 monoclonal antibody (6B4) 1820

antibody
Database link: P46013
Tested applications WB,ICC/IF
Recommended dilutions WB: 1:1000-5000 IF 1:2000-5000
Immunogen Recombinant construct containing the 2nd, 3rd and 4th ki-67 repeats of the human sequence (amino acids 1,111-1,490) expressed in and purified from E. coli.
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance
ki-67 is a widely used cell marker in research, particularly in the field of cell proliferation and cancer biology. It is a nuclear protein that is present during active phases of the cell cycle (G1, S, G2, and M phases) but absent in resting cells (G0 phase). As a marker of cellular proliferation, ki-67 is utilized to assess the growth fraction of a cell population and to quantify the proportion of actively dividing cells within a tissue or sample. Immunohistochemistry and immunofluorescence techniques employing antibodies targeting ki-67 are commonly employed to visualize and quantify its expression in tissues and cell cultures. Click for more on: cell markers and ki-67
Protein names Proliferation marker protein Ki-67 (Antigen identified by monoclonal antibody Ki-67) (Antigen KI-67) (Antigen Ki67)
Gene names MKI67,MKI67
Mass 358694Da
Function Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Subellular location Chromosome Nucleus Nucleus, nucleolus Note=Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
Structure Interacts with KIF15 (PubMed:10878014). Interacts (via the FHA domain) with NIFK (PubMed:11342549, PubMed:14659764, PubMed:16244663). Interacts with PPP1CC (PubMed:24867636). Component of a complex at least composed of ZNF335, HCFC1, CCAR2, EMSY, MKI67, RBBP5, ASH2L and WDR5; the complex is formed as a result of interactions between components of a nuclear receptor-mediated transcription complex and a histone methylation complex (PubMed:19131338). Interacts with ZNF335 (PubMed:19131338, PubMed:23178126).
Post-translational modification Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
Biotechnology Widely used as a marker to assess cell proliferation, as it is detected in the nucleus of proliferating cells only (PubMed:6339421, PubMed:21960707). In cancer research field for example, MKI67 is the most widely used marker for comparing proliferation between tumor samples (PubMed:21960707, PubMed:26680267).
Target Relevance information above includes information from UniProt accession: P46013
The UniProt Consortium

Data

Confcoal immunofluorescence image at high magnification of HeLa cell culture stained with mouse monoclonal antibody to Ki-67, 1820, in green, and costained with chicken polyclonal antibody to Lamin A/C in red. The antibody against Ki67 stains the nuclei of rapidly dividing cells within their nucleoli, but doesn't stain nearby quiescent cells. The antibody against Lamin A/C stains nuclear lamina. The blue is DAPI staining of nuclear DNA.

Western blot analysis of equal amounts of cell lysates using mouse mAb to Ki67, 1820, dilution 1:2,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of apparent molecular weight of 345kDa and 395kDa. Smaller proteolytic fragments of these isoforms are also invariably detected on the blot.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

There are 8 publications in our database for this antibody or clone. Here are the latest 5, for more click below.

pmid	title	authors	citation
27362226	Ki-67 acts as a biological surfactant to disperse mitotic chromosomes	Cuylen S, Blaukopf C, Politi AZ, Müller-Reichert T, Neumann B, Poser I, Ellenberg J, Hyman AA, Gerlich DW.	Nature. 2016 Jul 14;535(7611):308-12. doi: 10.1038/nature18610. Epub 2016 Jun 29.
23235554	Retrospective analysis of risk factors for central nervous system metastases in operable breast cancer: effects of biologic subtype and Ki67 overexpression on survival	Ishihara M, Mukai H, Nagai S, Onozawa M, Nihei K, Shimada T, Wada N.	Oncology. 2013;84(3):135-40. doi: 10.1159/000345321. Epub 2012 Dec 11.
22452635	Low endoglin vascular density and Ki67 index in Gleason score 6 tumours may identify prostate cancer patients suitable for surveillance	Josefsson A, Wikström P, Egevad L, Granfors T, Karlberg L, Stattin P, Bergh A.	Scand J Urol Nephrol. 2012 Aug;46(4):247-57. doi: 10.3109/00365599.2012.669791. Epub 2012 Mar 27.
20152769	Ki67 in breast cancer: prognostic and predictive potential	Yerushalmi R, Woods R, Ravdin PM, Hayes MM, Gelmon KA.	Lancet Oncol. 2010 Feb;11(2):174-83. doi: 10.1016/S1470-2045(09)70262-1.
19436038	Ki67 index, HER2 status, and prognosis of patients with luminal B breast cancer	Cheang MC, Chia SK, Voduc D, Gao D, Leung S, Snider J, Watson M, Davies S, Bernard PS, Parker JS, Perou CM, Ellis MJ, Nielsen TO.	J Natl Cancer Inst. 2009 May 20;101(10):736-50. doi: 10.1093/jnci/djp082. Epub 2009 May 12.