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mouse anti-Ki67 monoclonal antibody (5F86) 7961


Antibody summary

  • Mouse monoclonal to Ki67
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: mouse, rat
  • Isotype: IgG1
  • 100 µg, 25 µg, 1 mg
SKU: 7961parent Categories: , Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in







1 mg/mL



available sizes

1 mg, 100 µg, 25 µg

Available product – mouse anti-Ki67 monoclonal antibody (5F86) 7961

Database link:
mouse E9PVX6
rat Q5RJM0
Tested applications
Recommended dilutions
WB: 1:5000-10000 IF 1:2000-5000, IHC 1:1000
Recombinant construct containing amino acids 956-1322 of the mouse sequence XP_006507475 expressed in and purified from E. coli.
Size and concentration
25, 100, 1000µg and 1 mg/mL
Storage Instructions
2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS, 50% glycerol, 0.04% NaN3
affinity purified
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Ki67 is a widely used cell marker in research, particularly in the field of cell proliferation and cancer biology. It is a nuclear protein that is present during active phases of the cell cycle (G1, S, G2, and M phases) but absent in resting cells (G0 phase). As a marker of cellular proliferation, Ki67 is utilized to assess the growth fraction of a cell population and to quantify the proportion of actively dividing cells within a tissue or sample. Immunohistochemistry and immunofluorescence techniques employing antibodies targeting Ki67 are commonly employed to visualize and quantify its expression in tissues and cell cultures.

Click for more on: cell markers and ki67
Protein names
Proliferation marker protein Ki-67 (Antigen identified by monoclonal antibody Ki-67) (Antigen KI-67) (Antigen Ki67)
Gene names
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Subellular location
Chromosome Nucleus Nucleus, nucleolus Note=Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
Interacts with KIF15 (PubMed:10878014). Interacts (via the FHA domain) with NIFK (PubMed:11342549, PubMed:14659764, PubMed:16244663). Interacts with PPP1CC (PubMed:24867636). Component of a complex at least composed of ZNF335, HCFC1, CCAR2, EMSY, MKI67, RBBP5, ASH2L and WDR5; the complex is formed as a result of interactions between components of a nuclear receptor-mediated transcription complex and a histone methylation complex (PubMed:19131338). Interacts with ZNF335 (PubMed:19131338, PubMed:23178126).
Post-translational modification
Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
Widely used as a marker to assess cell proliferation, as it is detected in the nucleus of proliferating cells only (PubMed:6339421, PubMed:21960707). In cancer research field for example, MKI67 is the most widely used marker for comparing proliferation between tumor samples (PubMed:21960707, PubMed:26680267).
Target Relevance information above includes information from UniProt accession : P46013
The UniProt Consortium


Immunofluorescent analysis of HeLa cells stained with rabbit pAb to Ki67 7961, dilution 1:5,000 in red, and mouse monoclonal antibody to fibrillarin, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. The Ki67 protein accumulates in and around the nucleoli of interphase cells such as those on the right, and the nucleoli are revealed by the fibrillarin antibody. In contrast, cells in the quiescent G0 state such as those on the left are Ki67 negative but fibrillarin positive.
Western blot analysis of equal amounts of cell lysates using rabbit pAb to Ki67 7961, dilution 1:10,000, (green): [1] protein standard (red), [2] rapidly growing HeLa cell cultures, [3] rapidly growing HEK293 cell cultures. Strong double bands larger than the 250kDa standard correspond to full length 345kDa and 395kDa Ki67 isoforms, while smaller proteolytic fragments of these isoforms are also invariably detected on the blot.
Chromogenic immunostaining of a 4%.PFA fixed paraffin embedded rat testes section with mouse mAb to Ki67, 7961, dilution 1:2,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In testes, the 7961 antibody strongly labels the nuclei of spermatogenic cells. This antibody was not tested for staining NBF fixed tissues. Both rat and mouse tissues stain effectively with 7961. Mouse select image for larger view.


Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

27362226Ki-67 acts as a biological surfactant to disperse mitotic chromosomesCuylen S, Blaukopf C, Politi AZ, Müller-Reichert T, Neumann B, Poser I, Ellenberg J, Hyman AA, Gerlich DW.Nature. 2016 Jul 14;535(7611):308-12. doi: 10.1038/nature18610. Epub 2016 Jun 29.
23235554Retrospective analysis of risk factors for central nervous system metastases in operable breast cancer: effects of biologic subtype and Ki67 overexpression on survivalIshihara M, Mukai H, Nagai S, Onozawa M, Nihei K, Shimada T, Wada N.Oncology. 2013;84(3):135-40. doi: 10.1159/000345321. Epub 2012 Dec 11.
22452635Low endoglin vascular density and Ki67 index in Gleason score 6 tumours may identify prostate cancer patients suitable for surveillanceJosefsson A, Wikström P, Egevad L, Granfors T, Karlberg L, Stattin P, Bergh A.Scand J Urol Nephrol. 2012 Aug;46(4):247-57. doi: 10.3109/00365599.2012.669791. Epub 2012 Mar 27.
20152769Ki67 in breast cancer: prognostic and predictive potentialYerushalmi R, Woods R, Ravdin PM, Hayes MM, Gelmon KA.Lancet Oncol. 2010 Feb;11(2):174-83. doi: 10.1016/S1470-2045(09)70262-1.
19436038Ki67 index, HER2 status, and prognosis of patients with luminal B breast cancerCheang MC, Chia SK, Voduc D, Gao D, Leung S, Snider J, Watson M, Davies S, Bernard PS, Parker JS, Perou CM, Ellis MJ, Nielsen TO.J Natl Cancer Inst. 2009 May 20;101(10):736-50. doi: 10.1093/jnci/djp082. Epub 2009 May 12.
19141773Multi-institutional validation of the predictive value of Ki-67 labeling index in patients with urinary bladder cancerMargulis V, Lotan Y, Karakiewicz PI, Fradet Y, Ashfaq R, Capitanio U, Montorsi F, Bastian PJ, Nielsen ME, Müller SC, Rigaud J, Heukamp LC, Netto G, Lerner SP, Sagalowsky AI, Shariat SF.J Natl Cancer Inst. 2009 Jan 21;101(2):114-9. doi: 10.1093/jnci/djn451. Epub 2009 Jan 13.
7911472The expression of proliferation-dependent antigens during the lifespan of normal and progeroid human fibroblasts in cultureKill IR, Faragher RG, Lawrence K, Shall S.J Cell Sci. 1994 Feb;107 ( Pt 2):571-9. doi: 10.1242/jcs.107.2.571.
6339421Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferationGerdes J, Schwab U, Lemke H, Stein H.Int J Cancer. 1983 Jan 15;31(1):13-20. doi: 10.1002/ijc.2910310104.


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