ICC/IF image mouse anti Ki67 monoclonal antibody 5F86 7961

mouse anti-Ki67 monoclonal antibody (5F86) 7961

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to Ki67
Suitable for: WB, ICC/IF, IHC
Reacts with: mouse, rat
Isotype: IgG1
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 7961parent Categories: antibody, cell marker Tag: Ki-67

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	human, mouse, rat
available sizes	1 mg, 100 µg, 25 µg

mouse anti-Ki67 monoclonal antibody (5F86) 7961

antibody
Database link: mouse E9PVX6 rat Q5RJM0
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:5000-10000 IF 1:2000-5000, IHC 1:1000
Immunogen Recombinant construct containing amino acids 956-1322 of the mouse sequence XP_006507475 expressed in and purified from E. coli.
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance
ki-67 is a widely used cell marker in research, particularly in the field of cell proliferation and cancer biology. It is a nuclear protein that is present during active phases of the cell cycle (G1, S, G2, and M phases) but absent in resting cells (G0 phase). As a marker of cellular proliferation, ki-67 is utilized to assess the growth fraction of a cell population and to quantify the proportion of actively dividing cells within a tissue or sample. Immunohistochemistry and immunofluorescence techniques employing antibodies targeting ki-67 are commonly employed to visualize and quantify its expression in tissues and cell cultures. Click for more on: cell markers and ki-67
Protein names Proliferation marker protein Ki-67 (Antigen identified by monoclonal antibody Ki-67) (Antigen KI-67) (Antigen Ki67)
Gene names MKI67,MKI67
Mass 358694Da
Function FUNCTION: Protein that associates with the surface of mitotic chromosomes and acts both as a chromosome repellent during early mitosis and chromosome attractant during late mitosis (PubMed:27362226, PubMed:32879492, PubMed:35513709, PubMed:39153474). Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). During early mitosis, relocalizes from nucleoli to the chromosome surface where it forms extended brush structures that cover a substantial fraction of the chromosome surface (PubMed:27362226). The MKI67 brush structure prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). During mitotic anaphase, the MKI67 brush structure collapses and MKI67 switches from a chromosome repellent to a chromosome attractant to promote chromosome clustering and facilitate the exclusion of large cytoplasmic particles from the future nuclear space (PubMed:32879492, PubMed:39153474). Mechanistically, dephosphorylation during mitotic exit and simultaneous exposure of a conserved basic patch induce the RNA-dependent formation of a liquid-like condensed phase on the chromosome surface, promoting coalescence of neighboring chromosome surfaces and clustering of chromosomes (PubMed:39153474). Binds premature ribosomal RNAs during anaphase; promoting liquid-liquid phase separation (PubMed:28935370, PubMed:39153474). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization; it is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in mitotic chromosome (PubMed:24867636). {ECO:0000250\|UniProtKB:E9PVX6, ECO:0000269\|PubMed:10878551, ECO:0000269\|PubMed:24867636, ECO:0000269\|PubMed:27362226, ECO:0000269\|PubMed:28935370, ECO:0000269\|PubMed:32879492, ECO:0000269\|PubMed:35513709, ECO:0000269\|PubMed:39153474}.
Subellular location SUBCELLULAR LOCATION: Chromosome {ECO:0000269\|PubMed:15896774, ECO:0000269\|PubMed:22002106, ECO:0000269\|PubMed:27362226, ECO:0000269\|PubMed:32879492, ECO:0000269\|PubMed:35513709, ECO:0000269\|PubMed:39153474, ECO:0000269\|PubMed:9510506}. Nucleus {ECO:0000269\|PubMed:10878551, ECO:0000269\|PubMed:22002106}. Nucleus, nucleolus {ECO:0000269\|PubMed:10878551, ECO:0000269\|PubMed:22002106, ECO:0000269\|PubMed:2674163, ECO:0000269\|PubMed:8799815}. Note=During early mitosis, relocalizes from nucleoli to the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106). {ECO:0000269\|PubMed:15896774, ECO:0000269\|PubMed:22002106, ECO:0000269\|PubMed:27362226}.
Structure SUBUNIT: Interacts with KIF15 (PubMed:10878014). Interacts (via the FHA domain) with NIFK (PubMed:11342549, PubMed:14659764, PubMed:16244663). Interacts with PPP1CC (PubMed:24867636, PubMed:25012651). Component of a complex at least composed of ZNF335, HCFC1, CCAR2, EMSY, MKI67, RBBP5, ASH2L and WDR5; the complex is formed as a result of interactions between components of a nuclear receptor-mediated transcription complex and a histone methylation complex (PubMed:19131338). Interacts with ZNF335 (PubMed:19131338, PubMed:23178126). {ECO:0000269\|PubMed:10878014, ECO:0000269\|PubMed:11342549, ECO:0000269\|PubMed:14659764, ECO:0000269\|PubMed:16244663, ECO:0000269\|PubMed:19131338, ECO:0000269\|PubMed:23178126, ECO:0000269\|PubMed:24867636, ECO:0000269\|PubMed:25012651}.
Post-translational modification PTM: Hyperphosphorylated by CDK1 in mitosis; hyperphosphorylatiom prevents undergoing liquid-liquid phase separation (PubMed:10502411, PubMed:10653604, PubMed:25012651, PubMed:39153474). Dephosphorylated by PPP1CC at the onset of anaphase (PubMed:25012651). Dephosphorylated by protein phosphatase 2A (PP2A) at the onset of anaphase (By similarity). Dephosphorylation by protein phosphatase 2A (PP2A) and simultaneous exposure of the positively charged patch (CP) during mitotic exit induce the RNA-dependent formation of a liquid-like condensed phase on the chromosome surface (PubMed:39153474). {ECO:0000250\|UniProtKB:E9PVX6, ECO:0000269\|PubMed:10502411, ECO:0000269\|PubMed:10653604, ECO:0000269\|PubMed:25012651, ECO:0000269\|PubMed:39153474}.; PTM: Ubiquitinated by the APC/C complex after neuronal progenitors exit mitosis during brain development, leading to clearance from constitutive heterochromatin. {ECO:0000269\|PubMed:34942119}.
Domain DOMAIN: The positively charged patch (CP) region mediates liquid-liquid phase separation. {ECO:0000269\|PubMed:39153474}.
Biotechnology BIOTECHNOLOGY: Widely used as a marker to assess cell proliferation, as it is detected in the nucleus of proliferating cells only (PubMed:21960707, PubMed:6339421). In cancer research field for example, MKI67 is the most widely used marker for comparing proliferation between tumor samples (PubMed:21960707, PubMed:26680267). {ECO:0000269\|PubMed:6339421, ECO:0000303\|PubMed:21960707, ECO:0000303\|PubMed:26680267}.
Target Relevance information above includes information from UniProt accession: P46013
The UniProt Consortium

Data

Immunofluorescent analysis of HeLa cells stained with rabbit pAb to Ki67 7961, dilution 1:5,000 in red, and mouse monoclonal antibody to fibrillarin, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. The Ki67 protein accumulates in and around the nucleoli of interphase cells such as those on the right, and the nucleoli are revealed by the fibrillarin antibody. In contrast, cells in the quiescent G0 state such as those on the left are Ki67 negative but fibrillarin positive.

Western blot analysis of equal amounts of cell lysates using rabbit pAb to Ki67 7961, dilution 1:10,000, (green): [1] protein standard (red), [2] rapidly growing HeLa cell cultures, [3] rapidly growing HEK293 cell cultures. Strong double bands larger than the 250kDa standard correspond to full length 345kDa and 395kDa Ki67 isoforms, while smaller proteolytic fragments of these isoforms are also invariably detected on the blot.

Chromogenic immunostaining of a 4%.PFA fixed paraffin embedded rat testes section with mouse mAb to Ki67, 7961, dilution 1:2,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In testes, the 7961 antibody strongly labels the nuclei of spermatogenic cells. This antibody was not tested for staining NBF fixed tissues. Both rat and mouse tissues stain effectively with 7961.

Publications

pmid	title	authors	citation
27362226	Ki-67 acts as a biological surfactant to disperse mitotic chromosomes.	Sara Cuylen, Claudia Blaukopf, Antonio Z Politi, Thomas M�ller-Reichert, Beate Neumann, Ina Poser, Jan Ellenberg, Anthony A Hyman, Daniel W Gerlich	Nature 535:308-12
23235554	Retrospective analysis of risk factors for central nervous system metastases in operable breast cancer: effects of biologic subtype and Ki67 overexpression on survival.	Mikiya Ishihara, Hirofumi Mukai, Shunji Nagai, Masakatsu Onozawa, Keiji Nihei, Toshiyuki Shimada, Noriaki Wada	Oncology 84:135-40
22452635	Low endoglin vascular density and Ki67 index in Gleason score 6 tumours may identify prostate cancer patients suitable for surveillance.	Andreas Josefsson, Pernilla Wikstr�m, Lars Egevad, Torvald Granfors, Lars Karlberg, P�r Stattin, Anders Bergh	Scand J Urol Nephrol 46:247-57
20152769	Ki67 in breast cancer: prognostic and predictive potential.	Rinat Yerushalmi, Ryan Woods, Peter M Ravdin, Malcolm M Hayes, Karen A Gelmon	Lancet Oncol 11:174-83
19436038	Ki67 index, HER2 status, and prognosis of patients with luminal B breast cancer.	Maggie C U Cheang, Stephen K Chia, David Voduc, Dongxia Gao, Samuel Leung, Jacqueline Snider, Mark Watson, Sherri Davies, Philip S Bernard, Joel S Parker, Charles M Perou, Matthew J Ellis, Torsten O Nielsen	J Natl Cancer Inst 101:736-50

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.