mouse anti-HA tag monoclonal antibody (HA.C5) 2742

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to HA tag
Suitable for: WB,ICC/IF,ELISA
Reacts with: tagged fusion proteins
Isotype: IgG3
100 µg, 25 µg, 1 mg

available sizes

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SKU: 2742parent Categories: antibody, epitope tag Tag: HA tag

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG3
clonality	monoclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

mouse anti-HA tag monoclonal antibody (HA.C5) 2742

antibody
Database link: Sequence -YPYDVPDYA Derived from HA 118-127. Example strain H3N8 Q03909
Tested applications WB,ICC/IF,ELISA
Recommended dilutions WB: 1:1000-3000 ICC/IF: 1:500-2000 For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Immunogen YPYDVPDYA (HA) synthetic peptide conjugated to KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions -20°C for 2 years or more. °Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
Storage buffer PBS, pH 7.2, 0.05% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG3
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG3 - Isotype Control

target relevance

HA tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify HA tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, HA tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody.

Click for more on: epitope tags and HA tag

Biotechnology
The HA tag, derived from the human influenza hemagglutinin protein, is a widely employed tool in recombinant protein expression and has become an invaluable asset in molecular biology research. When incorporated at the N- or C-terminus of a target protein, the HA tag serves as a short, immunogenic peptide sequence, enabling efficient detection, purification, and characterization of the expressed protein. Its small size minimizes disruption to the target protein???s structure and function, making it suitable for a diverse range of applications. The HA tag is specifically recognized by commercially available high-affinity antibodies, facilitating the precise and sensitive detection of the tagged protein in techniques like Western blotting and immunofluorescence. Additionally, the HA tag is compatible with affinity chromatography, enabling straightforward and effective purification of the tagged protein from complex cellular extracts. This versatility has made the HA tag a valuable tool for studying protein interactions, localization, and post-translational modifications, providing researchers with valuable insights into cellular processes and molecular mechanisms across various fields of biology and biotechnology.

Data

1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2).

Publications

pmid	title	authors	citation
36321378	A NR2E1-interacting peptide of LSD1 inhibits the proliferation of brain tumour initiating cells.	Rong Hu, Umar Farook Shahul Hameed, Xiang Sun, Balakrishnan Shenbaga Moorthy, Wen Zhang, Philip D Jeffrey, Li Zhou, Xin Ma, Fangjin Chen, Jianfeng Pei, Pankaj K Giri, Yonggao Mou, Kunchithapadam Swaminathan, Ping Yuan	Cell Prolif 56:e13350
36119843	NDRG2 inhibits pyruvate carboxylase-mediated anaplerosis and combines with glutamine blockade to inhibit the proliferation of glioma cells.	Jiancai Wang, Xiang Sun, Jiayuan Wang, Kun Zhang, Yiyi Yuan, Yan Guo, Libo Yao, Xia Li, Lan Shen	Am J Cancer Res 12:3729-3744
36090478	Stabilization of DEPTOR sensitizes hypopharyngeal cancer to radiotherapy via targeting degradation.	Xuecen Wang, Zhirui Cao, Xin Yue, Tingyu Liu, Gesi Wen, Dongmei Jiang, Weijian Wu, Liyuan Le, Yan Wang, Chengtao Wang, Ziyang Wang, Meng Jin, Meiyan Zhu, Shasha He, Xiaoyue Zhang, Xianzhang Bu, Ran-Yi Liu, Zhenwei Peng, Yong Chen	Mol Ther Oncolytics 26:330-346
35847954	The NFAT3/RERG Complex in Luminal Breast Cancers Is Required to Inhibit Cell Invasion and May Be Correlated With an Absence of Axillary Lymph Nodes Colonization.	Lucie Coillard, Fr�d�ric Guaddachi, Ma�lle Ralu, Eva Brabencova, Christian Garbar, Armand Bensussan, Morgane Le Bras, Jacqueline Lehmann-Che, S�bastien Jauliac	Front Oncol 12:804868
35761309	Targeting ferroptosis with miR-144-3p to attenuate pancreatic ? cells dysfunction via regulating USP22/SIRT1 in type 2 diabetes.	Shanshan Zhang, Xiao Liu, Jihong Wang, Fengjuan Yuan, Yali Liu	Diabetol Metab Syndr 14:89

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.