| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG3 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| available sizes | 1 mg, 100 µg, 25 µg |
mouse anti-HA tag monoclonal antibody (HA.C5) 2742
$100.00 – $2,600.00
Antibody summary
- Mouse monoclonal to HA tag
- Suitable for: WB,ICC/IF,ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgG3
- 100 µg, 25 µg, 1 mg
mouse anti-HA tag monoclonal antibody (HA.C5) 2742
| antibody |
|---|
| Database link: Sequence -YPYDVPDYA Derived from HA 118-127. Example strain H3N8 Q03909 |
| Tested applications WB,ICC/IF,ELISA |
| Recommended dilutions WB: 1:1000-3000 ICC/IF: 1:500-2000 For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test. |
| Immunogen YPYDVPDYA (HA) synthetic peptide conjugated to KLH |
| Size and concentration 25, 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage buffer PBS, pH 7.2, 0.05% NaN3 |
| Purity affinity purified |
| Clonality monoclonal |
| Isotype IgG3 |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG3 - Isotype Control |
| target relevance |
|---|
| HA tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify HA tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, HA tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody. Click for more on: epitope tags and HA tag |
| Biotechnology The HA tag, derived from the human influenza hemagglutinin protein, is a widely employed tool in recombinant protein expression and has become an invaluable asset in molecular biology research. When incorporated at the N- or C-terminus of a target protein, the HA tag serves as a short, immunogenic peptide sequence, enabling efficient detection, purification, and characterization of the expressed protein. Its small size minimizes disruption to the target protein???s structure and function, making it suitable for a diverse range of applications. The HA tag is specifically recognized by commercially available high-affinity antibodies, facilitating the precise and sensitive detection of the tagged protein in techniques like Western blotting and immunofluorescence. Additionally, the HA tag is compatible with affinity chromatography, enabling straightforward and effective purification of the tagged protein from complex cellular extracts. This versatility has made the HA tag a valuable tool for studying protein interactions, localization, and post-translational modifications, providing researchers with valuable insights into cellular processes and molecular mechanisms across various fields of biology and biotechnology. |
Data
 |
| 1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2). |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 238 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
| pmid | title | authors | citation |
|---|---|---|---|
| 36321378 | A NR2E1-interacting peptide of LSD1 inhibits the proliferation of brain tumour initiating cells | Hu R, Hameed UFS, Sun X, Moorthy BS, Zhang W, Jeffrey PD, Zhou L, Ma X, Chen F, Pei J, Giri PK, Mou Y, Swaminathan K, Yuan P. | Cell Prolif. 2023 Jan;56(1):e13350. doi: 10.1111/cpr.13350. Epub 2022 Nov 2. |
| 36119843 | NDRG2 inhibits pyruvate carboxylase-mediated anaplerosis and combines with glutamine blockade to inhibit the proliferation of glioma cells | Wang J, Sun X, Wang J, Zhang K, Yuan Y, Guo Y, Yao L, Li X, Shen L. | Am J Cancer Res. 2022 Aug 15;12(8):3729-3744. eCollection 2022. |
| 36090478 | Stabilization of DEPTOR sensitizes hypopharyngeal cancer to radiotherapy via targeting degradation | Wang X, Cao Z, Yue X, Liu T, Wen G, Jiang D, Wu W, Le L, Wang Y, Wang C, Wang Z, Jin M, Zhu M, He S, Zhang X, Bu X, Liu RY, Peng Z, Chen Y. | Mol Ther Oncolytics. 2022 Aug 5;26:330-346. doi: 10.1016/j.omto.2022.08.002. eCollection 2022 Sep 15. |
| 35847954 | The NFAT3/RERG Complex in Luminal Breast Cancers Is Required to Inhibit Cell Invasion and May Be Correlated With an Absence of Axillary Lymph Nodes Colonization | Coillard L, Guaddachi F, Ralu M, Brabencova E, Garbar C, Bensussan A, Le Bras M, Lehmann-Che J, Jauliac S. | Front Oncol. 2022 Jun 30;12:804868. doi: 10.3389/fonc.2022.804868. eCollection 2022. |
| 35761309 | Targeting ferroptosis with miR-144-3p to attenuate pancreatic β cells dysfunction via regulating USP22/SIRT1 in type 2 diabetes | Zhang S, Liu X, Wang J, Yuan F, Liu Y. | Diabetol Metab Syndr. 2022 Jun 27;14(1):89. doi: 10.1186/s13098-022-00852-7. |
Protocols
| relevant to this product |
|---|
| Western blot ICC |
Documents
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