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mouse anti-GFAP monoclonal antibody (3E10) 9418

$100.00$2,600.00

Antibody summary

  • Mouse monoclonal to GFAP
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: human, mouse, rat
  • Isotype: IgG1
  • 100 µg, 25 µg, 1 mg
SKU: 9418parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, IHC, WB

reactivity

human, mouse, rat

available sizes

1 mg, 100 µg, 25 µg

mouse anti-GFAP monoclonal antibody (3E10) 9418

antibody
Database link:
human P14136
mouse P03995
rat P47819
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
WB: 1:1000 IF/ICC and IHC: 1:500
Immunogen
Recombinant human alpha-helical GFAP fragment expressed in and purified from E. coli
Size and concentration
25, 100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS, 50% glycerol, 0.04% NaN3
Purity
affinity purified
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Glial Fibrillary Acidic Protein (GFAP) is a crucial cell marker widely employed in neuroscience and neurobiology research. It is an intermediate filament protein primarily found in astrocytes, a type of glial cell in the central nervous system. GFAP serves as a reliable marker for identifying and characterizing astrocytes in various experimental models and tissues. Immunohistochemistry and immunofluorescence techniques using antibodies targeting GFAP enable researchers to visualize and quantify astrocytic populations, gaining insights into their distribution, morphology, and function in the brain. Moreover, GFAP antibodies are instrumental in studying astrocyte reactivity, a phenomenon occurring in response to brain injury or neurodegenerative diseases. As astrocytes play critical roles in neural development, neurotransmitter regulation, and maintenance of the blood-brain barrier, GFAP has proven indispensable in advancing our understanding of glial biology and its contribution to neurological disorders.

Click for more on: cell markers and GFAP
Protein names
Glial fibrillary acidic protein (GFAP)
Gene names
GFAP,GFAP
Protein family
Intermediate filament family
Mass
49880Da
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Subellular location
Cytoplasm Note=Associated with intermediate filaments.
Tissues
Expressed in cells lacking fibronectin.
Structure
Interacts with SYNM. ; [Isoform 2]: Interacts with PSEN1 (via N-terminus).
Post-translational modification
Phosphorylated by PKN1.
Involvement in disease
Alexander disease (ALXDRD) [MIM:203450]: A rare disorder of the central nervous system. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death within the first decade. Infants with Alexander disease develop a leukodystrophy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. Histologically, Alexander disease is characterized by Rosenthal fibers, homogeneous eosinophilic inclusions in astrocytes. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P14136
The UniProt Consortium

ICC/IF-image-mouse-anti-GFAP-monoclonal-antibody-3E10-9418
Immunofluorescent analysis of rat hippocampus section stained with mouse mAb to GFAP, 80010, dilution 1:500 in red, and costained with chicken pAb to MAP2, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with above antibodies. The GFAP antibody stains the network of astroglial cells while the antibody labels perikarya and dendrites of neurons.
IHC-image-mouse-anti-GFAP-monoclonal-antibody-3E10-9418
Chromogenic immunostaining of a formalin fixed paraffin embedded human brain cortex section with mouse mAb to GFAP, 80010, dilution 1:1,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In cortex, GFAP strongly labels astrocytes and glial cells. This antibody performs well in staining with 4% PFA or standard NBF fixed rat and human tissues. 80010 is our recommended and preferred clone for GFAP paraffin staining applications.
ICC/IF-image-mouse-anti-GFAP-monoclonal-antibody-3E10-9418
Immunofluorescent analysis of mouse hippocampus section stained with mouse mAb to GFAP, 80010, dilution 1:500 in green, and costained with chicken pAb to NF-L, dilution 1:2,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The GFAP antibody stains network of glial cells while the antibody labels axons and dendrites of neuronal cells.
WB-image-mouse-anti-GFAP-monoclonal-antibody-3E10-9418
Western blot analysis of equal amount of total proteins from different cell and tissue lysates using mouse mAb to GFAP, 80010, dilution 1:1,000 in green: [1] protein standard (red), [2] primary rat cortical neuron-glial cells, [3] rat brain, [4] mouse brain, [5] cow cortex, [6] cow cerebellum and [7] pig hippocampus. Bands around 50kDa marks correspond to GFAP protein and its alternative transcripts and fragments. The 80010 antibody has stronger reactivity with cow and pig GFAP as compare to rodent and reacts strongly with human native and recombinant GFAP (not shown). The same blot was probed with chicken pAb to UCHL1, a neuron cell marker, dilution 1:2,000, in red. The UCHL1 antibody reveals a 25kDa band corresponding to UCHL1 protein detected in all preparations.

Publications

pmidtitleauthorscitation
22497529A literature review of the feasibility of glial fibrillary acidic protein as a biomarker for stroke and traumatic brain injurySchiff L, Hadker N, Weiser S, Rausch C.Mol Diagn Ther. 2012 Apr 1;16(2):79-92. doi: 10.2165/11631580-000000000-00000.
17617407CNS injury, glial scars, and inflammation: Inhibitory extracellular matrices and regeneration failureFitch MT, Silver J.Exp Neurol. 2008 Feb;209(2):294-301. doi: 10.1016/j.expneurol.2007.05.014. Epub 2007 May 31.
11138011Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander diseaseBrenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE, Messing A.Nat Genet. 2001 Jan;27(1):117-20. doi: 10.1038/83679.
7193676Antibodies to neurofilament, glial filament, and fibroblast intermediate filament proteins bind to different cell types of the nervous systemYen SH, Fields KL.J Cell Biol. 1981 Jan;88(1):115-26. doi: 10.1083/jcb.88.1.115.
6799297An immunofluorescence microscopical study of the neurofilament triplet proteins, vimentin and glial fibrillary acidic protein within the adult rat brainShaw G, Osborn M, Weber K.Eur J Cell Biol. 1981 Dec;26(1):68-82.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

relevant to this product
Western blot
IHC
ICC
Batch Information
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