Immunohistochemistry (IHC) : Human colon carcinoma stained with anti-keratin 18 antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor glands.

mouse anti-Cytokeratin 18 monoclonal antibody (ZM205) 6138

$160.00 – $528.00

Antibody summary

Mouse monoclonal to Cytokeratin 18
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: Breast carcinoma
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6138parent Category: antibody Tags: anatomy, Cytokeratin 18, General & Epithelial Markers

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-Cytokeratin 18 monoclonal antibody ZM205 6138

antibody
Database link: human P05783
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Breast carcinoma
Immunogen Recombinant human KRT18 protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names Keratin, type I cytoskeletal 18 (Cell proliferation-inducing gene 46 protein) (Cytokeratin-18) (CK-18) (Keratin-18) (K18)
Gene names KRT18,KRT18 CYK18 PIG46
Protein family Intermediate filament family
Mass 48058Da
Function FUNCTION: Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. {ECO:0000250, ECO:0000269\|PubMed:15529338, ECO:0000269\|PubMed:16424149, ECO:0000269\|PubMed:17213200, ECO:0000269\|PubMed:7523419, ECO:0000269\|PubMed:8522591, ECO:0000269\|PubMed:9298992, ECO:0000269\|PubMed:9524113}.
Subellular location SUBCELLULAR LOCATION: Nucleus matrix {ECO:0000250\|UniProtKB:Q5BJY9}. Cytoplasm, perinuclear region. Nucleus, nucleolus {ECO:0000269\|PubMed:22002106}. Cytoplasm {ECO:0000250\|UniProtKB:Q5BJY9}.
Tissues TISSUE SPECIFICITY: Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. {ECO:0000269\|PubMed:17213200, ECO:0000269\|PubMed:2422083, ECO:0000269\|PubMed:2434380, ECO:0000269\|PubMed:2434381}.
Structure SUBUNIT: Heterotetramer of two type I and two type II keratins. KRT18 associates with KRT8 (PubMed:10852826, PubMed:24940650). Interacts with PLEC isoform 1C, when in a heterodimer with KRT8 (PubMed:24940650). Interacts with the thrombin-antithrombin complex (By similarity). Interacts with PNN and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with DNAJB6, TCHP and TRADD. Interacts with FAM83H (PubMed:23902688). Interacts with EPPK1 (By similarity). Interacts with PKP1 and PKP2 (PubMed:10852826). {ECO:0000250, ECO:0000250\|UniProtKB:P05784, ECO:0000269\|PubMed:10809736, ECO:0000269\|PubMed:10852826, ECO:0000269\|PubMed:10954706, ECO:0000269\|PubMed:11684708, ECO:0000269\|PubMed:15529338, ECO:0000269\|PubMed:15731013, ECO:0000269\|PubMed:23902688, ECO:0000269\|PubMed:24940650, ECO:0000269\|PubMed:8609167, ECO:0000269\|PubMed:9524113}.; SUBUNIT: (Microbial infection) Interacts with hepatitis C virus/HCV core protein. {ECO:0000269\|PubMed:15846844}.
Post-translational modification PTM: Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. {ECO:0000269\|PubMed:15368451, ECO:0000269\|PubMed:16424149, ECO:0000269\|PubMed:17213200, ECO:0000269\|PubMed:7523419, ECO:0000269\|PubMed:8609167, ECO:0000269\|PubMed:9524113}.; PTM: Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. {ECO:0000269\|PubMed:9298992}.; PTM: O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
Involvement in disease DISEASE: Cirrhosis (CIRRH) [MIM:215600]: A liver disease characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper. Clinical features include abdomen swelling, jaundice and pulmonary hypertension. {ECO:0000269\|PubMed:12724528, ECO:0000269\|PubMed:9011570}. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P05783
The UniProt Consortium

Data

Human colon carcinoma stained with anti-keratin 18 antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor glands.

Publications

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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.