| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, IHC, WB |
| available sizes | 1 mg, 100 µg, 25 µg |
mouse anti-Alpha-synuclein monoclonal antibody (2A7) 1129
$100.00 – $2,600.00
Antibody summary
- Mouse monoclonal to Alpha-synuclein
- Suitable for: WB, ICC/IF, IHC
- Reacts with: human, mouse, rat
- Isotype: IgG1
- 100 µg
mouse anti-Alpha-synuclein monoclonal antibody (2A7) 1129
| target relevance |
|---|
| Alpha-synuclein is a crucial protein involved in synaptic function and neurotransmitter release, primarily found in presynaptic terminals of neurons. In research, alpha-synuclein has gained significant attention as a potential cell marker for neurodegenerative diseases, particularly Parkinson's disease. The aggregation of alpha-synuclein into insoluble fibrils is a hallmark of Parkinson's pathology, and its presence in Lewy bodies is a diagnostic feature of the disease. Therefore, alpha-synuclein is utilized as a marker to identify and study Parkinson's disease-related pathology in postmortem brain tissues and experimental models. Antibodies targeting alpha-synuclein are extensively employed in immunohistochemistry and western blotting to visualize and quantify its expression levels in various brain regions and cellular compartments. Click for more on: cell markers and alpha synuclein |
| Protein names Alpha-synuclein (Non-A beta component of AD amyloid) (Non-A4 component of amyloid precursor) (NACP) |
| Gene names SNCA,SNCA NACP PARK1 |
| Protein family Synuclein family |
| Mass 14460Da |
| Function Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:28288128, PubMed:30404828, PubMed:20798282, PubMed:26442590). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590). |
| Subellular location Cytoplasm Membrane Nucleus Synapse Secreted Cell projection, axon Note=Membrane-bound in dopaminergic neurons (PubMed:15282274). Expressed and colocalized with SEPTIN4 in dopaminergic axon terminals, especially at the varicosities (By similarity). |
| Tissues Highly expressed in presynaptic terminals in the central nervous system. Expressed principally in brain. |
| Structure Soluble monomer. Homotetramer (PubMed:21841800). A dynamic intracellular population of tetramers and monomers coexists normally and the tetramer plays an essential role in maintaining homeostasis (PubMed:21841800). Interacts with UCHL1 (By similarity). Interacts with phospholipase D and histones. Interacts (via N-terminus) with synphilin-1/SNCAIP; this interaction promotes formation of SNCA inclusions in the cytoplasm (PubMed:19762560). Interacts with CALM1 (PubMed:23607618). Interacts with STXBP1; this interaction controls SNCA self-replicating aggregation (PubMed:27597756). Interacts with SNARE components VAMP2 and SNAP25; these interactions allows SNARE complex assembly and integrity (PubMed:20798282). Interacts with RPH3A and RAB3A (PubMed:15207266). Interacts with SERF1A; this interaction promotes the aggregation of SNCA (PubMed:22854022, PubMed:31034892). Interacts with SEPTIN4 (By similarity). |
| Post-translational modification Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress. ; Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.; Ubiquitinated. The predominant conjugate is the diubiquitinated form. ; Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. |
| Target Relevance information above includes information from UniProt accession : P37840 |
| The UniProt Consortium |
Data
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| Immunofluorescent analysis of rat cerebellum section stained with mouse mAb to alpha-synuclein 1129, dilution 1:1,000, in red, and costained with rabbit pAb to GFAP 2539 dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells. |
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| Cortex of a patient with Parkinson's disease (PD) stained with 1129, 1:1,000 dilution. Antibody revealed with horse radish peroxidase and DAB. The Lewy bodies and other typical inclusions of PD are seen in brown. Mouse select image for larger view. |
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| Chromogenic immunostaining of a NBF fixed paraffin embedded human cerebellum section with mouse mAb to alpha-synuclein, 1129, dilution 1:1,000, detected in DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. The alpha-synuclein antibody synaptic regions in the granular and molecular layers of the cerebellum. This antibody performs well in testing with both 4% PFA and standard NBF fixed tissues. Mouse select image for larger view. |
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| Immunofluorescent analysis of rat hippocampus section costained with mouse mAb to alpha-synuclein 1129, dilution 1:1,000, in green, and chicken pAb to MeCP2 dilution 1:2,000 in red). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45 µM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, and the MeCP2 antibody stains the nuclei of neuronal cells. Mouse select image for larger view. |
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| Immunofluorescent analysis of rat olfactory bulb section costained with mouse mAb to a-synuclein 1129, dilution 1:1,000 in red, and rabbit pAb to GFAP 2539, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous backbone of astroglial cells. Mouse select image for larger view. |
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| Various truncated and mutant construct were applied to PVDF membranes in about of 400ng of each protein. These were the first 60 amino acids of human https://encorbio.com/product/mca-2a7/alpha-synuclein (1-60), amino acids 61 to 140 (61-140), full length but incorporating the A30P and A53T mutations seen associated with familial forms of Parkinson's disease (A30P/A53T), full length but with the central NAC region of amino acids 61-95 missing (d61-95) and finally full length alpha synuclein. The strips of PVDF were probed with 1129, another EnCor antibody to alpha-synuclein, the Santa Cruz monoclonal antibody 211, the Santa Cruz rabbit polyclonal (R-SC) and EnCor's rabbit polyclonal (R-EnCor, RPCA-aSyn). The epitopes for 1129 and are clearly in the NAC region from 61-95, while the 211 antibody epitope is within the C-terminal region from 95-140 (in fact the epitope has been shown to be aminoacids 120-125). Both rabbit antibodies bind constructs including amino acids 61-140, but do not appear to bind amino acids 1-60, suggesting that this region has low immunogenicity. Mouse select image for larger view. |
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| Western (left) and Ponceau S stained blots (right) of recombinant full length human alpha, beta and α-synuclein in lanes 2, 3 and 4 respectively. 1129 reacts strongly with alpha-synuclein and shows no reaction with the other proteins. Lane 1 shows molecular weight standards of indicated molecular weight. |
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| Western blot analysis of different tissue lysates using mAb to alpha-synuclein 1129, dilution 1:1,000 in green. [1] protein standard in red, [2] whole rat brain lysate, [3] rat spinal cord lysate. The strong band at about 15kDa corresponds to alpha-synuclein protein. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 7 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
| pmid | title | authors | citation |
|---|---|---|---|
| 23255825 | Naturally occurring α-synuclein autoantibody levels are lower in patients with Parkinson disease | Besong-Agbo D, Wolf E, Jessen F, Oechsner M, Hametner E, Poewe W, Reindl M, Oertel WH, Noelker C, Bacher M, Dodel R. | Neurology. 2013 Jan 8;80(2):169-75. doi: 10.1212/WNL.0b013e31827b90d1. Epub 2012 Dec 19. |
| 20974939 | αβγ-Synuclein triple knockout mice reveal age-dependent neuronal dysfunction | Greten-Harrison B, Polydoro M, Morimoto-Tomita M, Diao L, Williams AM, Nie EH, Makani S, Tian N, Castillo PE, Buchman VL, Chandra SS. | Proc Natl Acad Sci U S A. 2010 Nov 9;107(45):19573-8. doi: 10.1073/pnas.1005005107. Epub 2010 Oct 25. |
| 15451224 | Alpha-synuclein locus duplication as a cause of familial Parkinson's disease | Chartier-Harlin MC, Kachergus J, Roumier C, Mouroux V, Douay X, Lincoln S, Levecque C, Larvor L, Andrieux J, Hulihan M, Waucquier N, Defebvre L, Amouyel P, Farrer M, Destée A. | Lancet. 2004 Sep 25-Oct 1;364(9440):1167-9. doi: 10.1016/S0140-6736(04)17103-1. |
| 9462735 | Ala30Pro mutation in the gene encoding alpha-synuclein in Parkinson's disease | Krüger R, Kuhn W, Müller T, Woitalla D, Graeber M, Kösel S, Przuntek H, Epplen JT, Schöls L, Riess O. | Nat Genet. 1998 Feb;18(2):106-8. doi: 10.1038/ng0298-106. |
| 9197268 | Mutation in the alpha-synuclein gene identified in families with Parkinson's disease | Polymeropoulos MH, Lavedan C, Leroy E, Ide SE, Dehejia A, Dutra A, Pike B, Root H, Rubenstein J, Boyer R, Stenroos ES, Chandrasekharappa S, Athanassiadou A, Papapetropoulos T, Johnson WG, Lazzarini AM, Duvoisin RC, Di Iorio G, Golbe LI, Nussbaum RL. | Science. 1997 Jun 27;276(5321):2045-7. doi: 10.1126/science.276.5321.2045. |
Protocols
| relevant to this product |
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| Western blot IHC ICC |
Documents
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