Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human solitary fibrous tumor stained with anti-ALDH1A1 monoclonal antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic/nuclear staining of tumor cells

mouse anti-ALDH1A1 monoclonal antibody (ZM71) 6015

$160.00 – $528.00

Antibody summary

Mouse monoclonal to ALDH1A1
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: Solitary fibrous tumor
Visualization: Cytoplasmic and nuclear
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6015parent Category: antibody Tags: ALDH1A1, anatomy, Soft Tissue Pathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-ALDH1A1 monoclonal antibody ZM71 6015

antibody
Database link: human P00352
Tested applications IHC
Recommended dilutions As directed
Immunogen Recombinant full-length human ALDH1A1 protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names Aldehyde dehydrogenase 1A1 (EC 1.2.1.19) (EC 1.2.1.28) (EC 1.2.1.3) (EC 1.2.1.36) (3-deoxyglucosone dehydrogenase) (ALDH-E1) (ALHDII) (Aldehyde dehydrogenase family 1 member A1) (Aldehyde dehydrogenase, cytosolic) (Retinal dehydrogenase 1) (RALDH 1) (RalDH1)
Gene names ALDH1A1,ALDH1A1 ALDC ALDH1 PUMB1
Protein family Aldehyde dehydrogenase family
Mass 54862Da
Function Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid (PubMed:19296407, PubMed:12941160, PubMed:15623782, PubMed:17175089, PubMed:26373694, PubMed:25450233). Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid (By similarity). This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic (By similarity). Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification (PubMed:19296407, PubMed:12941160, PubMed:15623782). Functions also downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins (PubMed:17175089). Has also an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission (By similarity).
Catalytic activity Reaction=an aldehyde + H2O + NAD(+) = a carboxylate + 2 H(+) + NADH; Xref=Rhea:RHEA:16185, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17478, ChEBI:CHEBI:29067, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.2.1.3; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16186; Evidence=; Reaction=all-trans-retinal + H2O + NAD(+) = all-trans-retinoate + 2 H(+) + NADH; Xref=Rhea:RHEA:42080, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17898, ChEBI:CHEBI:35291, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.2.1.36; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42081; Evidence=; Reaction=9-cis-retinal + H2O + NAD(+) = 9-cis-retinoate + 2 H(+) + NADH; Xref=Rhea:RHEA:42084, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:78273, ChEBI:CHEBI:78630; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42085; Evidence=; Reaction=11-cis-retinal + H2O + NAD(+) = 11-cis-retinoate + 2 H(+) + NADH; Xref=Rhea:RHEA:47132, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16066, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:87435; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47133; Evidence=; Reaction=13-cis-retinal + H2O + NAD(+) = 13-cis-retinoate + 2 H(+) + NADH; Xref=Rhea:RHEA:67332, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:45487, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:169952; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67333; Evidence=; Reaction=3-deoxyglucosone + H2O + NAD(+) = 2-dehydro-3-deoxy-D-gluconate + 2 H(+) + NADH; Xref=Rhea:RHEA:67244, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:57990, ChEBI:CHEBI:60777; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67245; Evidence=; Reaction=(E)-4-hydroxynon-2-enal + H2O + NAD(+) = (E)-4-hydroxynon-2-enoate + 2 H(+) + NADH; Xref=Rhea:RHEA:67248, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:58968, ChEBI:CHEBI:142920; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67249; Evidence=; Reaction=H2O + malonaldehyde + NAD(+) = 3-oxopropanoate + 2 H(+) + NADH; Xref=Rhea:RHEA:67252, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33190, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:566274; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67253; Evidence=; Reaction=H2O + hexanal + NAD(+) = 2 H(+) + hexanoate + NADH; Xref=Rhea:RHEA:67276, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17120, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:88528; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67277; Evidence=; Reaction=H2O + NAD(+) + propanal = 2 H(+) + NADH + propanoate; Xref=Rhea:RHEA:67256, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17153, ChEBI:CHEBI:17272, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67257; Evidence=; Reaction=acetaldehyde + H2O + NAD(+) = acetate + 2 H(+) + NADH; Xref=Rhea:RHEA:25294, ChEBI:CHEBI:15343, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.2.1.3; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:25295; Evidence=; Reaction=benzaldehyde + H2O + NAD(+) = benzoate + 2 H(+) + NADH; Xref=Rhea:RHEA:11840, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16150, ChEBI:CHEBI:17169, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.2.1.28; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11841; Evidence=; Reaction=4-aminobutanal + H2O + NAD(+) = 4-aminobutanoate + 2 H(+) + NADH; Xref=Rhea:RHEA:19105, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:58264, ChEBI:CHEBI:59888; EC=1.2.1.19; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19106; Evidence=;
Pathway Cofactor metabolism; retinol metabolism.
Subellular location Cytoplasm, cytosol Cell projection, axon
Tissues Expressed by erythrocytes (at protein level).
Structure Homotetramer (By similarity). Interacts with PRMT3; the interaction is direct, inhibits ALDH1A1 aldehyde dehydrogenase activity and is independent of the methyltransferase activity of PRMT3 (PubMed:33495566).
Post-translational modification The N-terminus is blocked most probably by acetylation.
Target Relevance information above includes information from UniProt accession: P00352
The UniProt Consortium