mouse anti-6X His tag monoclonal antibody (7B8) 1955

Price range: $100.00 through $2,600.00

Antibody summary

Mouse monoclonal to 6X His tag
Suitable for: ELISA
Reacts with: tagged fusion proteins
Isotype: IgG2b
100 µg, 25 µg, 1 mg

available sizes

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SKU: 1955parent Categories: antibody, epitope tag Tag: 6XHis tag

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2b
clonality	monoclonal
concentration	1 mg/mL
applications	ELISA
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

mouse anti-6X His tag monoclonal antibody (7B8) 1955

antibody
Database link: 6x His tag sequence HHHHHH
Tested applications ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with 6xHIS as determined by ELISA and western blot. Optimal working dilutions should be determined experimentally by the investigator
Immunogen Highly purified HHHHHH conjugated with KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG2b
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG2b - Isotype Control

target relevance
6X His tag
Protein names Hexahistidine tag
Alternative names 6xHis tag, His tag, Hexa-His tag, Polyhistidine tag, His6 tag, 6His tag
Function The 6×His tag enables purification and detection of recombinant proteins through coordination of histidine residues with immobilized metal ions such as nickel, cobalt, copper, or zinc.
Description The 6×His tag is a synthetic peptide tag consisting of six consecutive histidine residues. It is one of the most widely used affinity tags for recombinant protein purification and detection.
Related entities relationship: binds_to; entity: Nickel ion relationship: binds_to; entity: Ni-NTA relationship: binds_to; entity: Cobalt affinity resin relationship: modified_form_of; entity: 8×His tag relationship: modified_form_of; entity: 10×His tag
Keywords Affinity tag, Protein purification, IMAC, Recombinant protein, Protein detection
Sequence HHHHHH
Structure The 6×His tag consists of six consecutive histidine residues (HHHHHH) and is typically fused to the N- or C-terminus of recombinant proteins.
Function The histidine residues coordinate transition metal ions, enabling affinity purification and detection of tagged proteins.
Purification His-tagged proteins are commonly purified using immobilized metal affinity chromatography (IMAC), including Ni-NTA and cobalt-based resins.
Detection His-tagged proteins can be detected using anti-His antibodies, anti-polyhistidine antibodies, nickel conjugates, or IMAC reagents.
Research relevance The 6×His tag is one of the most widely used recombinant protein tags because of its small size, minimal impact on protein folding, and compatibility with rapid purification workflows.

Data

The expression of 6XHIS was determined by Western blot. CHO cells were transfected with pcDNA3-12 Tags (CHO-12 Tag). Whole cell lysates of CHO-12 Tag, and CHO were loaded at 2µg and 0.2µg then incubated with mouse anti-6X His tag monoclonal antibody (7B8) 1955 at 1:10000 (dilution 0.1 µg/mL) for 1h at room temperature.

FAQ & Publications

Frequently Asked Questions

What applications is the mouse anti-6X His tag monoclonal antibody (7B8) validated for?

This monoclonal antibody has been tested and validated for use in ELISA and Western blot applications to detect 6X His tagged fusion proteins.

How should the mouse anti-6X His tag antibody be stored to maintain stability?

The antibody should be stored refrigerated at 2-8°C in its provided PBS buffer (pH 7.2) containing 0.09% sodium azide to maintain its stability.

What is the host species and isotype of this anti-6X His tag antibody?

The antibody is a mouse monoclonal of the IgG2b isotype.

What is the immunogen used to generate the mouse anti-6X His tag monoclonal antibody (7B8)?

The immunogen is a highly purified 6X His peptide (HHHHHH) conjugated to keyhole limpet hemocyanin (KLH).

Publications

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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.