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Leptospira IgM ELISA kit-SERION classic ESR125M

$355.00

Summary

Virion/Serion Diagnostic Kit for research use (RUO)
Leptospira IgM ELISA Kit
Suitable for IgM detection
Ready-to-use
96 tests

SKU: ESR125M Category: Virion/Serion kits Tags: ELISA, IgM, Leptospira

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	Leptospira reactive IgM
species reactivity	Leptospira
applications	ELISA
assay type	Indirect & quantitative
available sizes	96 tests

Leptospira IgM ELISA Kit ESR125M

Assay type
Indirect ELISA

Research area
Infectious Disease

Sample type
Serum, plasma, whole blood

Notes
Pretreatment of samples with RF-Absorbent (Z200) is recommended for use with IgM ELISA kits to eliminate presence of sample rheumatoid factors and possible false negative results.

Components

Break apart microtiter test strips each with antigen coated single wells	8 x 12 (96 Total)
Standard serum (ready-to-use)	2 x 2 mL
Negative control serum (ready-to-use)	2 mL
Anti-human-IgM-conjugate (ready-to-use)	13 mL
Washing solution concentrate (sufficient for 1000ml)	33.3 mL
Dilution buffer	2 x 50 mL
Stopping solution	15 mL
Substrate (ready-to-use)	13 mL
Quality control certificate with standard curve and evaluation table	1

Storage
Store at 2-8°C.

Associated products
Leptospira biflexa Antigen (BA125VS)
Leptospira IgG Control Serum (BC125G)
Leptospira IgM Control Serum (BC125M)
Leptospira IgG ELISA Kit (ESR125G)
Leptospira IgM ELISA Kit (ESR125M)

target relevance

human antibody anti-Leptospira

Leptospira

Description
Spirochete bacterium that causes leptospirosis

Structure
Leptospira is a genus of thin, highly motile, Gram-negative spirochete bacteria belonging to the family Leptospiraceae. Members of the genus possess a characteristic tightly coiled helical morphology with hooked ends, often described as question mark-shaped. The bacteria are widely distributed in nature and are maintained in the kidneys of a broad range of wild and domestic animals, including rodents, cattle, pigs, dogs, and wildlife species. Pathogenic Leptospira species are shed into the environment through urine and can survive for extended periods in moist soil and freshwater. Human infection occurs through direct contact with infected animals or exposure to water, soil, or food contaminated with infected urine. Leptospira organisms are capable of penetrating mucous membranes and damaged skin, disseminating through the bloodstream, and infecting multiple organ systems.

Diagnosis
The diagnosis of leptospirosis is performed through serological, molecular, microbiological, and microscopic methods. The microscopic agglutination test (MAT) is considered the reference serological method and is widely used to detect antibodies against Leptospira. ELISA-based assays are commonly employed for screening and diagnosis due to their simplicity and sensitivity. Molecular methods such as polymerase chain reaction (PCR) provide rapid detection of Leptospira DNA during the early phase of infection before antibodies become detectable. Culture of Leptospira from blood, cerebrospinal fluid, or urine is possible but technically demanding and may require prolonged incubation. Laboratory findings often include elevated liver enzymes, renal dysfunction, thrombocytopenia, and inflammatory markers. Early diagnosis is essential because prompt antimicrobial therapy can reduce disease severity and complications.

Symptoms
Leptospirosis typically develops following an incubation period of approximately two to thirty days, most commonly seven to twelve days after exposure. The disease often begins abruptly with fever, chills, severe headache, muscle aches, particularly involving the calves and lower back, fatigue, nausea, vomiting, and conjunctival suffusion. Many infections are mild and self-limiting; however, severe disease may develop and is known as Weil's disease. This severe form is characterized by jaundice, acute kidney injury, liver dysfunction, hemorrhage, meningitis, myocarditis, respiratory distress, and multi-organ failure. Pulmonary involvement can result in life-threatening pulmonary hemorrhage syndrome. Without prompt treatment, severe leptospirosis can be fatal, particularly in elderly individuals and patients with significant organ involvement.

Data

FAQ & Publications

Frequently Asked Questions

What type of samples can be used with the Leptospira IgM ELISA Kit ESR125M?

The kit is compatible with serum, plasma, and whole blood samples for IgM detection.

Is there a recommended pretreatment for samples to improve assay specificity?

Yes, pretreatment with RF-Absorbent Z200 is recommended to remove IgM rheumatoid factors from samples, reducing the risk of false negative results.

What is the storage condition for the Leptospira IgM ELISA Kit ESR125M?

The kit components should be stored at 2-8°C to maintain stability and performance.

Which Leptospira species does this ELISA kit target for IgM detection?

The kit targets IgM antibodies reactive to Leptospira species, which include pathogenic and saprophytic spirochaete bacteria.

How many tests are included in one kit of the Leptospira IgM ELISA Kit ESR125M?

Each kit contains sufficient reagents for 96 tests using break apart microtiter test strips.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
ESR125M protocol

Documents

#
EP0081	Safety Data Sheet	QC certificate
EP0133	Safety Data Sheet	QC certificate

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Leptospira IgM ELISA kit-SERION classic ESR125M

RF-Absorbent Z200 for use with IgM ELISA kits

Data

FAQ & Publications

Protocols

Documents

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Reviews

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